Biodegradation of ochratoxin A by<i>Alcaligenes faecalis</i>isolated from soil

Zhang, H.H.; Wang, Yuxin; Zhao, Chen; Wang, J.; Zhang, X.L.

doi:10.1111/jam.13537

Cited by 53 publications

(26 citation statements)

References 35 publications

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“…The low OTA reduction efficiency in CFEs compared to the degradation by actinobacteria was in accordance with the results of El Khoury et al [17] and suggests that degradation may be achieved by intracellular enzymes. Such intracellular enzymes with strong OTA degrading ability into OTα have been described for Alcaligenes faecalis, a Gram-negative bacteria isolated from soil [51], but not for actinobacteria, as far as we know. Some actinobacteria of other genera such as Brevibacterium are able to degrade OTA into OTα and L-β-phenylalanine, probably through the action of a carboxypeptidase [25].…”

Section: Discussionmentioning

confidence: 82%

Minimizing Ochratoxin A Contamination through the Use of Actinobacteria and Their Active Molecules

Campos-Avelar

Noue

Durand

et al. 2020

Toxins

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Ochratoxin A (OTA) is a secondary metabolite produced by fungal pathogens such as Penicillium verrucosum, which develops in food commodities during storage such as cereals, grapes, and coffee. It represents public health concerns due to its genotoxicity, carcinogenicity, and teratogenicity. The objective of this study was to evaluate the ability of actinobacteria and their metabolites to degrade OTA and/or to decrease its production. Sixty strains of actinobacteria were tested for their ability to prevent OTA formation by in vitro dual culture assays or with cell free extracts (CFEs). In dual culture, 17 strains strongly inhibited fungal growth, although it was generally associated with an increase in OTA specific production. Seventeen strains inhibited OTA specific production up to 4% of the control. Eleven actinobacteria CFEs reduced OTA specific production up to 62% of the control, while no substantial growth inhibition was observed except for two strains up to 72% of the control. Thirty-three strains were able to degrade OTA almost completely in liquid medium whereas only five were able to decrease it on solid medium, and two of them reduced OTA to an undetectable amount. Our results suggest that OTA decrease could be related to different strategies of degradation/metabolization by actinobacteria, through enzyme activities and secretion of secondary metabolites interfering with the OTA biosynthetic pathway. CFEs appeared to be ineffective at degrading OTA, raising interesting questions about the detoxification mechanisms. Common degradation by-products (e.g., OTα or L-β-phenylalanine) were searched by HPLC-MS/MS, however, none of them were found, which implies a different mechanism of detoxification and/or a subsequent degradation into unknown products.

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Section: Discussionmentioning

confidence: 82%

Minimizing Ochratoxin A Contamination through the Use of Actinobacteria and Their Active Molecules

Campos-Avelar

Noue

Durand

et al. 2020

Toxins

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“…Bacillus pumilus (bacterium) [122] Hydrolysis, spontaneous decarboxylation Lactonase [123] Hydrolysis, spontaneous decarboxylation Lactonase [124] Iso-DOM-3-GlcA (95) and iso-DOM-8-GlcA (96) Isomerization and glucuronization RLM [71] Fomannoxin (40) Fomannoxin acid (59) and fomannoxin acid β-glucoside (145) Oxidation and glycosylation Cell cultures of Pinus sylvestris [44] Fumonisin B1 (115) Hydrolyzed fumonisin B1 = Aminopentol 1 (AP1, 116) and 2-keto AP1 (132)…”

Section: Hydrolysis Spontaneous Decarboxylationmentioning

confidence: 99%

“…OTA (21) can be produced by various Aspergillus and Penicillium species. The most effective detoxification for OTA (21) was hydrolysis with transformed products as OTα (118) and phenylalanine (119) by several bacterial and fungal species ( Figure S57) [94][95][96][97][98][145][146][147]. The main transformation pathways of OTs are as follows [16].…”

Section: Detoxification Of Ochratoxinsmentioning

confidence: 99%

“…(i) Hydrolysis: hydrolysis of OTs include degradations of amide and ester bonds. OTA (21) could be hydrolyzed into OTα (118) and phenylalanine (Phe, 119) by bacteria such as Bacillus amyloliquefaciens [98], Alcaligenes faecalis [145] and Brevibacterium sp. [146], as well as fungi such as Aspergillus niger [97] and A. tubingensis [147].…”

Section: Detoxification Of Ochratoxinsmentioning

confidence: 99%

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Detoxification of Mycotoxins through Biotransformation

Yin

et al. 2020

Toxins

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Mycotoxins are toxic fungal secondary metabolites that pose a major threat to the safety of food and feed. Mycotoxins are usually converted into less toxic or non-toxic metabolites through biotransformation that are often made by living organisms as well as the isolated enzymes. The conversions mainly include hydroxylation, oxidation, hydrogenation, de-epoxidation, methylation, glycosylation and glucuronidation, esterification, hydrolysis, sulfation, demethylation and deamination. Biotransformations of some notorious mycotoxins such as alfatoxins, alternariol, citrinin, fomannoxin, ochratoxins, patulin, trichothecenes and zearalenone analogues are reviewed in detail. The recent development and applications of mycotoxins detoxification through biotransformation are also discussed.

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“…Degradation experiments were carried out in 250-mL Erlenmeyer flasks containing 100 mL sterilized medium supplemented with azo dye, and the inoculation size (OD 600 0.8) was 2 % (v/v). To characterize the degradation efficiency of strain LJ-3, the effects of static anoxic/shaking conditions (160 rpm), carbon sources (glucose, maltose, sucrose, lactose, dextrin, fructose, and xylose), nitrogen sources (yeast extract, beef extract, peptone, urea, glycine, NH 4 NO 3 , and NaNO 3 ), initial pH (4.0, 5.0, 6.0, 7.0, 8.0, 9.0, 10.0, 11.0, 12.0), incubation temperature (15,20,25,30,35,40, 45, 50 °C), and salinity (5,10,15,20,25,30,50, 80 g L −1 NaCl) on the degradation of 100 mg L -1 Acid Scarlet 3R were individually monitored. To find out maximum dye degrading ability of strain LJ-3, different concentrations of Acid Scarlet 3R (100, 200, 400, 600, 800, 1000, 1500, 2000 mg L −1 ) were respectively tested.…”

Section: Degradation Experimentsmentioning

confidence: 99%

Biodegradation of Acid Scarlet 3R by a New Salt-tolerant Strain Alcaligenes faecalis LJ-3: Character, Enzyme and Kinetics Analysis

Song

Liu

Zhou

et al. 2018

Chem.Biochem.Eng.Q.

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Alcaligenes faecalis LJ-3 is highly efficient in degrading various azo dyes (100 mg L -1 ). Almost 100 % degradation was observed within 16 h at the initial Acid Scarlet 3R concentrations of 1000 mg L -1 under the optimal conditions, which were: 1 g dextrin L -1 , 3 g yeast extract L -1 , NaCl ≤ 30 g L -1 , pH 8.0 and 35-45 ºC. Azoreductase, laccase and NADH-DCIP (nicotinamide adenine dinucleotide-dichlorophenol indophenols) reductase were induced during the degradation of Acid Scarlet 3R. Kinetics study of degradation experiments approximated the first-order reaction. The maximum rate (V max ) and substrate affinity constant (K s ) were found to be 115.90 mg L -1 h -1 and 1193.23 mg L -1 , respectively, using Michaelis-Menten kinetics. This work provides new data characterizing Acid Scarlet 3R degradation by bacteria, as well as practical application potential in biological treatment of industrial effluents containing various azo dyes.

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Biodegradation of ochratoxin A byAlcaligenes faecalisisolated from soil

Cited by 53 publications

References 35 publications

Minimizing Ochratoxin A Contamination through the Use of Actinobacteria and Their Active Molecules

Minimizing Ochratoxin A Contamination through the Use of Actinobacteria and Their Active Molecules

Detoxification of Mycotoxins through Biotransformation

Biodegradation of Acid Scarlet 3R by a New Salt-tolerant Strain Alcaligenes faecalis LJ-3: Character, Enzyme and Kinetics Analysis

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