Biodegradation of Oil by a Newly Isolated Strain Acinetobacter junii WCO-9 and Its Comparative Pan-Genome Analysis

Abstract

Waste oil pollution and the treatment of oily waste present a challenge, and the exploitation of microbial resources is a safe and efficient method to resolve these problems. Lipase-producing microorganisms can directly degrade waste oil and promote the degradation of oily waste and, therefore, have very significant research and application value. The isolation of efficient oil-degrading strains is of great practical significance in research into microbial remediation in oil-contaminated environments and for the enrichment of the microbial lipase resource library. In this study, Acinetobacter junii WCO-9, an efficient oil-degrading bacterium, was isolated from an oil-contaminated soil using olive oil as the sole carbon source, and its enzyme activity of ρ-nitrophenyl decanoate (ρ-NPD) decomposition was 3000 U/L. The WCO-9 strain could degrade a variety of edible oils, and its degradation capability was significantly better than that of the control strain, A junii ATCC 17908. Comparative pan-genome and lipid degradation pathway analyses indicated that A. junii isolated from the same environment shared a similar set of core genes and that the species accumulated more specific genes that facilitated resistance to environmental stresses under different environmental conditions. WCO-9 has accumulated a complete set of oil metabolism genes under a long-term oil-contamination environment, and the compact arrangement of abundant lipase and lipase chaperones has further strengthened the ability of the strain to survive in such environments. This is the main reason why WCO-9 is able to degrade oil significantly more effectively than ATCC 17908. In addition, WCO-9 possesses a specific lipase that is not found in homologous strains. In summary, A. junii WCO-9, with a complete triglyceride degradation pathway and the specific lipase gene, has great potential in environmental remediation and lipase for industry.

Unraveling the Gut Microbiome–Diet Connection: Exploring the Impact of Digital Precision and Personalized Nutrition on Microbiota Composition and Host Physiology

“…Thanks to our in-depth analysis, we were able to monitor changes in the gut microbiome at the level of microbial species. In particular, in our study, we identified several microbes that exhibited significant changes in abundance following the dietary intervention, including Acinetobacter junii, which, according to recent studies, is involved in the metabolism of fats [ 68 ]. The observed higher consumption of high-fat foods, including ice cream, Parmesan cheese, and oily fish, can thus explain the correlation with diet.…”

Genome characterisation and comparative analysis of Schaalia dentiphila sp. nov. and its subspecies, S. dentiphila subsp. denticola subsp. nov., from the human oral cavity

“…However, a consensus on the protein sequence identity threshold for pangenome analysis remains elusive. While many studies default to a 50% blastp threshold [ 64 – 67 ], our analyses (both BPGA and Roary) employed a stringent 95% blastp threshold [ 54 , 68 ], which ensures that only sequences with very high similarity are deemed homologous. Given the close evolutionary ties and subtle differences expected between NCTC 9931 and C24, a higher threshold may help in discerning highly conserved core genes from accessory and unique genes, minimizing false positives [ 68 , 69 ].…”

Metagenomic peek into a corn mummy

“…53 C. acnes is also known to occur in human skin 54 and soil 55 , but as we do not see a historical pattern of DNA damage for this bacterium, it is more likely to be contemporary human contamination. Bacteria with less than 1000 reads but at least 0.1% abundance may be related to soil, rhizosphere ( A. junii , 56 C. gambrini , 57 K. rhizophila , 58 Methylosinus sp. C49, 59 M. luteus , 60 M. osloensis , 61 O. iheyensis , 62 P. merdae 63 ) or human intestinal tract, faeces ( Alistipes spp., 64,65 B. caccae , 66 D. succinatiphilus , 67 D. longicatena , 68 F. prausnitzii , 69 O. splanchnicus , 70 P. vulgatus 71 ).…”