Biodegradation of organic compounds of molasses melanoidin (MM) from biomethanated distillery spent wash (BMDS) during the decolourisation by a potential bacterial consortium

Abstract

Molasses melanoidin (MM) is a major pollutant in biomethanated distillery spent wash (BMDS) due to its recalcitrant properties. The 75% colour and 71% COD of MM (1,000 ppm) were reduced with developed bacterial consortium comprising Proteus mirabilis (IITRM5; FJ581028), Bacillus sp. (IITRM7; FJ581030), Raoultella planticola (IITRM15; GU329705) and Enterobacter sakazakii (IITRM16, FJ581031) in the ratio of 4:3:2:1 within 10 days at optimized nutrient. Bacterial consortium showed manganese peroxidase and laccase activity during MM decolourisation. The dominant growth of R. planticola and E. sakazakii was noted in consortium during MM decolourisation. The comparative GC-MS analysis of extracted compounds of control and degraded samples showed that most of the compounds present in control were completely utilized by bacterial consortium along with production of some metabolites. The developed bacterial consortium could be a tool for the decolourisation and degradation of melanoidin containing BMDS.

Evaluation of Press Mud, Vinasse Powder and Extraction Sludge with Ethanol in a Pyrolysis Process

“…Two different solid vinasse samples were studied. The first one was an extraction sludge ( Figure 2b) from a protein denaturation process described by Yadav and Chandra [29], in which ethanol and vinasse are mixed in a 500 mL separating funnel in equal volumetric proportions [30]. The mixture was stirred, and the solids were allowed to settle for two hours.…”

Covalently Immobilized Laccase for Decolourization of Glucose-Glycine Maillard Products as Colourant of Distillery Wastewater

“…He was used as carrier gas with a flow rate of 1 mL/min. In full-scan mode, the electron ionization (EI) mass spectra were recorded in the range of 30-550 (m/z) at 70 eV [7]. The degradation compounds were identified using Turbomass Gold Software-NIST-Mass Spectral Library.…”

“…To eliminate the persistent colour, a variety of microbial isolates including bacteria, fungi, yeast etc. have been tested [6,7]. The treatment is, however, limited by factors such as the requirement of easily digestible carbon sources (like sucrose, glucose), slow degradation rate, reactor clogging and loss of the extracellular enzymes and mediators with discharged wastewater.…”

Biodegradation of Melanoidin from Distillery Effluent: Role of Microbes and Their Potential Enzymes

“…Physico-chemical treatment Enzymatic treatment Adsorption [38] Coagulation [42] Flocculation [43] Oxidation process [44] Membrane treatment [46] Evaporation [47][48][49] Combustion [50] Peroxidases Oxidoreductases Cellulolytic enzymes cyanidase Proteases Amylases etc. [122][123][124][125][126][127] Biological treatment Aerobic treatment Anaerobic treatment/ Reactors [53][54][55][56][57][58][59][60][61][62][63][64][65][66][67] Bacterial treatment [69][70][71][72][73][74][75][76][77][78][79][80][81][82][83][84][85][86][87]…”