Biodegradation of p-nitrophenol by Rhodococcus sp. CN6 with high cell surface hydrophobicity

Zhang, Jingshun; Sun, Zhangli; Li, Yingying; Peng, Xiang; Li, Wen; Yan, Yanchun

doi:10.1016/j.jhazmat.2008.07.018

Cited by 61 publications

(22 citation statements)

References 31 publications

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“…The cell surface hydrophobicity (CSH) of the selected strain was measured during the growth on DEHP according to the microbial adherence to hydrocarbon (MATH) method proposed by Rosenberg et al (1980) and modified by Zhang et al (2009). The calculation is briefly described as below:…”

Section: The Variation Of Cell Surface Hydrophobicitymentioning

confidence: 99%

Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples

Ren

Yang

Nahurira

et al. 2016

Environ Sci Pollut Res

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Bacterial strain YC-RL4, capable of utilizing phthalic acid esters (PAEs) as the sole carbon source for growth, was isolated from petroleum-contaminated soil. Strain YC-RL4 was identified as Mycobacterium sp. by 16S rRNA gene analysis and Biolog tests. Mycobacterium sp. YC-RL4 could rapidly degrade dibutyl phthalate (DBP), diethyl phthalate (DEP), dimethyl phthalate (DMP), dicyclohexyl phthalate (DCHP), and di-(2-ethylhexyl) phthalate (DEHP) under both individual and mixed conditions, and all the degradation rates were above 85.0 % within 5 days. The effects of environmental factors which might affect the degrading process were optimized as 30 °C and pH 8.0. The DEHP metabolites were detected by HPLC-MS and the degradation pathway was deduced tentatively. DEHP was transformed into phthalic acid (PA) via mono (2-ethylhexyl) phthalate (MEHP) and PA was further utilized for growth via benzoic acid (BA) degradation pathway. Cell surface hydrophobicity (CSH) assays illuminated that the strain YC-RL4 was of higher hydrophobicity while grown on DEHP and CSH increased with the higher DEHP concentration. The degradation rates of DEHP by strain YC-RL4 in different environmental samples was around 62.0 to 83.3 % and strain YC-RL4 survived well in the soil sample. These results suggested that the strain YC-RL4 could be used as a potential and efficient PAE degrader for the bioremediation of contaminated sites.

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Section: The Variation Of Cell Surface Hydrophobicitymentioning

confidence: 99%

Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples

Ren

Yang

Nahurira

et al. 2016

Environ Sci Pollut Res

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“…Introduction of growth substrates into the culture is necessary for biomass production and, if its structure is similar to xenobiotic, for induction of enzymes required for cometabolite transformation (Cornelissen and Sijm 1996). For cometabolic degradation of mononitrophenols simple sources of carbon, as alcohol, organic acids, aminoacids or carbohydrates were frequently used (Karim and Gupta 2002;Kulkarni and Chaudhari 2006;Qui et al 2007;Zhang et al 2008). Degradation of these nitro-compounds was also observed in the presence of phenol, which was not only the growth substrate for bacterial strain but also, according to its similar structure to nitrophenol, induced enzymes of nitrophenol degradation pathway (Cho et al 2000).…”

Section: Cometabolic Degradation Of Mononitrophenols By Stenotrophomomentioning

confidence: 99%

“…The intensive studies on isolation and identification of microorganisms being able to degrade mononitrophenols have last since early 1960s of the twentieth century. Nitroaromatic contaminants are unfamiliar substances for microorganisms thus formation of new metabolic pathways enable its use as growth substrates and introduction into central metabolic pathways is not easy (Navratilova et al 2005;Zhao and Ward 2000;Zhang et al 2008).…”

Section: Introductionmentioning

confidence: 99%

“…In experiments on cometabolic biodegradation of xenobiotics as growth substrates simple organic compounds, as aminoacids or carbohydrates are frequently used (Karim and Gupta 2002;Qui et al 2007;Zhao and Ward 2000;Zhang et al 2008). For example Qui et al (2007) during research on cometabolic degradation of 4-nitrophenol used glucose as a growth substrate.…”

Section: Introductionmentioning

confidence: 99%

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Enhanced biotransformation of mononitrophenols by Stenotrophomonas maltophilia KB2 in the presence of aromatic compounds of plant origin

Greń

Wojcieszyńska

Guzik

et al. 2009

World J Microbiol Biotechnol

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Stenotrophomonas maltophilia KB2 used in this study is known to metabolise broad range of aromatic compounds including phenol, some chloro and methylphenols, benzoic acids, catochols and others. To study the applicability of the strain for degradation of mononitrophenols in monosubstrate as well as cometabolic systems its degradation potential in the presence of mononitrophenols or different aromatic compounds of plant origin was tested. Stenotrophomonas maltophilia KB2 strain was not able to degrade any of mononitrophenols used in the single substrate experiments. Effect of additional carbon source on nitrophenols degradation revealed that presence of benzoate, 4-hydroxybenzoate or 3,4-dixydroxybenzoate stimulate transformation of 2-nitrophenol, 3-nitrophenol as well as 4-nitrophenol. Depending on growth substrate and mononitrophenol used, decrease in cometabolite concentration was from 25 to 45%. Obtained results suggest that Stenotrophomonas maltophilia KB2 strain could be potentially used for cometabolic degradation of nitrophenols in the presence of aromatic acids, for the bioremediation of contaminated sites.

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“…These data suggest that azoreductases should be reclassified as NAD(P)H quinone oxidoreductases (NQOs). NQOs have also been shown to be important for bacterial metabolism of some substrates, e.g., p-nitrophenol (Zhang et al, 2009). Even though mammalian NQOs have very low sequence identity with the bacterial enzymes (less than 15% between paAzoR1 and either human NQO1 or NQO2), they also reduce quinone and azo compounds (Cui et al, 1995).…”

Section: Introductionmentioning

confidence: 99%

Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases

et al. 2010

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Azoreductases are involved in the bioremediation by bacteria of azo dyes found in waste water. In the gut flora, they activate azo pro-drugs, which are used for treatment of inflammatory bowel disease, releasing the active component 5-aminosalycilic acid. The bacterium P. aeruginosa has three azoreductase genes, paAzoR1, paAzoR2 and paAzoR3, which as recombinant enzymes have been shown to have different substrate specificities. The mechanism of azoreduction relies upon tautomerisation of the substrate to the hydrazone form. We report here the characterization of the P. aeruginosa azoreductase enzymes, including determining their thermostability, cofactor preference and kinetic constants against a range of their favoured substrates. The expression levels of these enzymes during growth of P. aeruginosa are altered by the presence of azo substrates. It is shown that enzymes that were originally described as azoreductases, are likely to act as NADH quinone oxidoreductases. The low sequence identities observed among NAD(P)H quinone oxidoreductase and azoreductase enzymes suggests convergent evolution.

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Biodegradation of p-nitrophenol by Rhodococcus sp. CN6 with high cell surface hydrophobicity

Cited by 61 publications

References 31 publications

Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples

Biodegradation of phthalic acid esters by a newly isolated Mycobacterium sp. YC-RL4 and the bioprocess with environmental samples

Enhanced biotransformation of mononitrophenols by Stenotrophomonas maltophilia KB2 in the presence of aromatic compounds of plant origin

Reaction mechanism of azoreductases suggests convergent evolution with quinone oxidoreductases

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