Biodegradation of pentaerythritol tetranitrate (PETN) by anaerobic consortia from a contaminated site

Li, Zhuang; Gui, Lai; Gillham, Robert W.

doi:10.1016/j.chemosphere.2012.04.062

Cited by 12 publications

(8 citation statements)

References 15 publications

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“…These changes in nitrate and nitrite can be correlated with the sequential reductive denitration processes that are bases for PETN biodegradation and some demineralization evidence. PETN underwent sequential reductive denitration processes, releasing nitrite in each denitration step, which was subsequently reduced to ammonia ( 34 ). On the other hand, release of NO2 group from nitroaromatic such as TNT, a positive evidence of demineralization has previously been reported.…”

Section: Discussionmentioning

confidence: 99%

Kinetic parameters and nitrate, nitrite changes in bioremediation of Toxic Pentaerythritol Tetranitrate (PETN) contaminated soil

et al. 2017

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BackgroundCleanup of areas contaminated by explosives is a public health concern. Some explosives can be carcinogenic in humans. Pentaerythritol Tetranitrate (PETN), a powerful explosive with very low water solubility, can be easily transported to ground waters.ObjectiveThis study was conducted to determine the removal efficiencies of PETN from soil by bioremediation, and obtain kinetic parameters of biological process.MethodsThis experimental study was conducted at the Environmental Health Engineering Lab (Isfahan University of Medical Sciences, Isfahan, Iran) in 2015–2016. In the present work, bioremediation of the explosive-polluted soils by PETN in anaerobic-aerobic landfarming method was performed. The influence of seeding and biosurfactant addition on bioremediation was also evaluated. The data were analyzed using Microsoft Excel software.ResultsThe results show that, as the initial concentration of PETN increased, the lag phase was increased and the specific growth rate was increased up to 0.1/day in concentration of 50 mg/kg, and then it was decreased to 0.04/day. Subsequent decreases in specific growth rate can cause substrate inhibition. Seeding causes decrease in lag phase significantly. Biosurfactant addition had little to no impact on the length of lag phase, but biosurfactant plus seeding can increase the growth rate to 0.2/day, however, inhibitory effect of the initial concentration was started in very high concentration of PETN (150 mg/kg).ConclusionBiosurfactant addition and seeding together have an impressive effect on biodegradation of PETN, furthermore seeding can enhance active microbial consortium and biosurfactant can improve the poor aqueous solubility of PETN, therefore making the substrate more accessible.

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Section: Discussionmentioning

confidence: 99%

Kinetic parameters and nitrate, nitrite changes in bioremediation of Toxic Pentaerythritol Tetranitrate (PETN) contaminated soil

et al. 2017

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“…Interestingly, the same enzyme can also metabolize TNT (French et al 1998), and as stated above, OYE enzymes are common in bacteria, yeast, plants, and nematodes (Williams and Bruce 2002). Another study showed that PETN can be degraded under anaerobic conditions by microbial enrichment cultures (Zhuang et al 2012). Nevertheless, it is not clear whether PETN is persistent in soils, and investigation of its environmental fate is scarce and contradictory.…”

Section: Responsible Editor: Zhihong Xumentioning

confidence: 92%

Persistence of pentolite (PETN and TNT) in soil microcosms and microbial enrichment cultures

Arbeli

García-Bonilla

Pardo

et al. 2016

Environ Sci Pollut Res

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Pentolite is a mixture (1:1) of 2,4,6-trinitrotoluene (TNT) and pentaerythritol tetranitrate (PETN), and little is known about its fate in the environment. This study was aimed to determine the dissipation of pentolite in soils under laboratory conditions. Microcosm experiments conducted with two soils demonstrated that dissipation rate of PETN was significantly slower than that of TNT. Interestingly, the dissipation of PETN was enhanced by the presence of TNT, while PETN did not enhanced the dissipation of TNT. Pentolite dissipation rate was significantly faster under biostimulation treatment (addition of carbon source) in soil from the artificial wetland, while no such stimulation was observed in soil from detonation field. In addition, the dissipation rate of TNT and PETN in soil from artificial wetland under biostimulation was significantly faster than the equivalent abiotic control, although it seems that non-biological processes might also be important for the dissipation of TNT and PETN. Transformation of PETN was also slower during establishment of enrichment culture using pentolite as the sole nitrogen source. In addition, transformation of these explosives was gradually reduced and practically stopped after the forth cultures transfer (80 days). DGGE analysis of bacterial communities from these cultures indicates that all consortia were dominated by bacteria from the order Burkholderiales and Rhodanobacter. In conclusion, our results suggest that PETN might be more persistent than TNT.

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“…To achieve this goal, it is crucial to adopt e cient, economic, and environmentally friendly technologies, such as bioremediation. Despite its recalcitrance and persistence, several in vitro studies have shown that PETN is susceptible to bacterial transformation under aerobic and anaerobic conditions (Binks et al 1996;Zhuang et al 2012;Avellaneda et al 2020). The most detailed studies on aerobic PETN transformation were carried out with Enterobacter cloacae PB2 and Agrobacterium radiobacter (Binks et al 1996; White et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

Bacteria isolated from explosive contaminated environments transform pentaerythritol tetranitrate (PETN) under aerobic and anaerobic conditions

Avila-Arias,

Casallas,

Arbeli

et al. 2023

Letters in Applied Microbiology

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Pentaerythritol tetranitrate (PETN) is a nitrate ester explosive that may be persistent, with scarce reports on its environmental fate and impacts. Our main objective was to isolate and characterize bacteria that transform PETN under aerobic and anaerobic conditions. Biotransformation of PETN (100 mg L−1) was evaluated using mineral medium with (M+C) and without (M-C) additional carbon source under aerobic conditions, and with additional carbon source under anaerobic conditions. Here we report on the isolation of 12 PETN transforming cultures (4 pure and 8 co-cultures) from environmental samples collected at an explosive manufacturing plant. The highest transformation of PETN was observed for cultures in M+C under aerobic conditions, reaching up to 91±2% in 2 days. Under this condition, PETN biotransformation was observed in conjunction with the release of nitrites and bacterial growth. No substantial transformation of PETN (<45%) was observed during 21 days in M-C under aerobic conditions. Under anaerobic conditions, five cultures could transform PETN (up to 52±13%) as the sole nitrogen source, concurrent with the formation of two unidentified metabolites. PETN-transforming cultures belonged to Alphaproteobacteria, Betaproteobacteria, Gammaproteobacteria and Actinobacteria. In conclusion, we isolated 12 PETN-transforming cultures belonging to diverse taxa suggesting that PETN transformation is phylogenetically widespread.

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Biodegradation of pentaerythritol tetranitrate (PETN) by anaerobic consortia from a contaminated site

Cited by 12 publications

References 15 publications

Kinetic parameters and nitrate, nitrite changes in bioremediation of Toxic Pentaerythritol Tetranitrate (PETN) contaminated soil

Kinetic parameters and nitrate, nitrite changes in bioremediation of Toxic Pentaerythritol Tetranitrate (PETN) contaminated soil

Persistence of pentolite (PETN and TNT) in soil microcosms and microbial enrichment cultures

Bacteria isolated from explosive contaminated environments transform pentaerythritol tetranitrate (PETN) under aerobic and anaerobic conditions

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