Biodegradation of perylene and benzo [ghi] perylene (5-6 rings) using yeast consortium : Kinetic study, enzyme analysis and degradation pathway

Mandal, Sanjeeb Kumar; Das, Nilanjana

doi:10.22438/jeb/39/1/mrn-540

Cited by 17 publications

(5 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Yadav and Loper in 1999 [61] pointed out that CYP is relevant to achieve the assimilation and degradation of both PAHs and alkanes, whereas Smith et al, in 2004 [62] confirmed that these compounds may be used as carbon sources. Additionally, Mandal and Das in 2016 and 2018 [57,63,64] reported that two different yeast consortiums, YC02 (integrated by Hanseniaspora opuntiae NS02, D. hansenii NS03, and Hanseniaspora valbyensis NS04) and YC04 (Rhodotorula sp NS01, D. hansenii NS03, and Hanseniaspora valbyensis NS04) metabolize perylene and benzo[ghi]perylene, respectively. D. hansenii is a yeast commonly found in marine environments because of its tolerance to high salt levels and its ability to grow in a wide range of carbon sources [65,66].…”

Section: Discussionmentioning

confidence: 99%

DhDIT2 Encodes a Debaryomyces hansenii Cytochrome P450 Involved in Benzo(a)pyrene Degradation—A Proposal for Mycoremediation

Padilla-Garfias

Sánchez

Calahorra

et al. 2022

JoF

View full text Add to dashboard Cite

Pollutants, such as polycyclic aromatic hydrocarbons (PAHs), e.g., benzo(a)pyrene (BaP), are common components of contaminating mixtures. Such compounds are ubiquitous, extremely toxic, and they pollute soils and aquatic niches. The need for new microorganism-based remediation strategies prompted researchers to identify the most suitable organisms to eliminate pollutants without interfering with the ecosystem. We analyzed the effect caused by BaP on the growth properties of Candida albicans, Debaryomyces hansenii, Rhodotorula mucilaginosa, and Saccharomyces cerevisiae. Their ability to metabolize BaP was also evaluated. The aim was to identify an optimal candidate to be used as the central component of a mycoremediation strategy. The results show that all four yeast species metabolized BaP by more than 70%, whereas their viability was not affected. The best results were observed for D. hansenii. When an incubation was performed in the presence of a cytochrome P450 (CYP) inhibitor, no BaP degradation was observed. Thus, the initial oxidation step is mediated by a CYP enzyme. Additionally, this study identified the D. hansenii DhDIT2 gene as essential to perform the initial degradation of BaP. Hence, we propose that D. hansenii and a S. cerevisiae expressing the DhDIT2 gene are suitable candidates to degrade BaP in contaminated environments.

show abstract

Section: Discussionmentioning

confidence: 99%

DhDIT2 Encodes a Debaryomyces hansenii Cytochrome P450 Involved in Benzo(a)pyrene Degradation—A Proposal for Mycoremediation

Padilla-Garfias

Sánchez

Calahorra

et al. 2022

JoF

View full text Add to dashboard Cite

show abstract

“…Benzo [ghi]perylene (BghiP) exemplifies a fascinating class of highly conjugated polyaromatic compounds formed by condensing benzenoid units and a vital group of fluorescent perylene dyes (Raouafi and Aloui, 2019). BghiP is a high molecular weight PAH compound with six benzene rings that is highly recalcitrant to degradation (Mandal and Das, 2018).…”

Section: Pahsmentioning

confidence: 99%

Comprehensive review of polycyclic aromatic hydrocarbons in water sources, their effects and treatments

Mojiri

Zhou

Ohashi

et al. 2019

Science of The Total Environment

441

154

View full text Add to dashboard Cite

Polycyclic aromatic hydrocarbons (PAHs) are principally derived from the incomplete combustion of fossil fuels. This study investigated the occurrence of PAHs in aquatic environments around the world, their effects on the environment and humans, and methods for their removal. Polycyclic aromatic hydrocarbons have a great negative impact on the environment and humans, and can even cause cancer in humans. Use of good methods and equipment are essential to monitoring PAHs, and GC/MS and HPLC are usually used for their analysis in aqueous solutions. In aquatic environments, the PAHs concentrations range widely from 0.03 ng/L (seawater; Southeastern Japan Sea, Japan) to 8,310,000 ng/L (Domestic Wastewater Treatment Plant, Siloam, South Africa). Moreover, bioaccumulation of ∑16PAHs in fish has been reported to range from 11.2 ng/L (Cynoscion guatucupa, South Africa) to 4207.5 ng/L (Saurida undosquamis, Egypt). Several physical/chemical and biological techniques have been reported to treat water contaminated by PAHs, but adsorption and combined treatment methods have shown better removal performance, with some methods removing up to 99.99% of PAHs.

show abstract

“…Fungi often co-metabolize these organic pollutants, by utilizing other available carbon sources such as plant or bacterial exudates or plant debris [25]. Fungi can typically degrade monoaromatic pollutants, but certain fungal species have been shown to also co-metabolize more complex compounds (e.g., PAHs with up to six benzene rings) [30].…”

Section: Mechanisms Of Degradation In Fungal-bacterial Systemsmentioning

confidence: 99%

“…Unlike bacteria, fungi transform PAHs by co-metabolism using different fungal enzymes (cytochrome P450 monooxygenase enzymes, lignin peroxidases, manganese peroxidases, and laccases) depending on species and growth conditions [119]. Typically, fungi can degrade four to fivebenzene-ring PAHs, although a yeast consortium (Rhodotorula sp., Debaryomyces hansenii, and Hanseniaspora valbyensis) was able to degrade benzo[ghi]perylene (a six-benzene-ring PAH) [30].…”

Section: Polycyclic Aromatic Hydrocarbons (Pahs)mentioning

confidence: 99%

Potential use of fungal-bacterial co-cultures for the removal of organic pollutants

Espinosa-Ortiz

Rene

Gerlach

2021

Critical Reviews in Biotechnology

View full text Add to dashboard Cite

Fungi and bacteria coexist in a wide variety of natural and artificial environments which can lead to their association and interaction -ranging from antagonism to cooperation -that can affect survival, colonization, spatial distribution and stress resistance of the interacting partners. The use of polymicrobial cultivation approaches has facilitated a more thorough understanding of microbial dynamics in mixed microbial communities, such as those composed of fungi and bacteria, and their influence on ecosystem functions. Mixed (multi-domain) microbial communities exhibit unique associations and interactions that could result in more efficient systems for the degradation and removal of organic pollutants. Several previous studies have reported enhanced biodegradation of certain pollutants when using combined fungal-bacterial treatments compared to pure cultures or communities of either fungi or bacteria (single domain systems). This article reviews: (i) the mechanisms of pollutant degradation that can occur in fungal-bacterial systems (e.g., co-degradation, production of secondary metabolites, enhancement of degradative enzyme production, and transport of bacteria by fungal mycelia); (ii) case studies using fungal-bacterial co-cultures for the removal of various organic pollutants (synthetic dyes, polycyclic aromatic hydrocarbons, pesticides and other trace or volatile organic compounds) in different environmental matrices (e.g., water, gas/vapors, soil); (iii) the key aspects of engineering artificial fungal-bacterial co-cultures, and (iv) the current challenges and future perspectives of using fungal-bacterial co-cultures for environmental remediation.

show abstract

Biodegradation of perylene and benzo [ghi] perylene (5-6 rings) using yeast consortium : Kinetic study, enzyme analysis and degradation pathway

Abstract: Aim :Methodology :

Cited by 17 publications

References 10 publications

DhDIT2 Encodes a Debaryomyces hansenii Cytochrome P450 Involved in Benzo(a)pyrene Degradation—A Proposal for Mycoremediation

DhDIT2 Encodes a Debaryomyces hansenii Cytochrome P450 Involved in Benzo(a)pyrene Degradation—A Proposal for Mycoremediation

Comprehensive review of polycyclic aromatic hydrocarbons in water sources, their effects and treatments

Potential use of fungal-bacterial co-cultures for the removal of organic pollutants

Contact Info

Product

Resources

About