Biodegradation of petroleum by Klebsiella pneumoniae isolated from drilling fluid

Özyürek, Sezen Bilen; Bilkay, Işıl Seyis

doi:10.1007/s13762-017-1581-y

Cited by 20 publications

(8 citation statements)

References 35 publications

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“…It has also been determined in previous studies that Klebsiella pneumoniae and Pannonibacter phragmitetus, which formed the consortium 2, were effective in the biodegradation of various organic environmental pollutants. In a previous study, it was shown that K. pneumoniae had an effect on short-, medium-and longchain n-alkanes via enzyme systems and can be used in bioremediation studies [24]. However, You et al [28] clearly showed that K. pneumoniae only biodegraded hydrocarbons in the range of C 10 -C 14 in diesel.…”

Section: Biodegradation Of N-alkane Fractions In Petroleummentioning

confidence: 99%

“…The experiments were performed in triplicate. The method was carried out as described previously in Bilen Ozyurek and Bilkay [24].…”

Section: Petroleum Biodegradation Assaysmentioning

confidence: 99%

“…To evaporate DCM, Erlenmeyer flasks were placed in the water bath (Memmert, Schwabach, Germany) and left for half an hour at 90 °C. The biodegradation of petroleum was calculated as the difference between initial and final concentrations of petroleum [24,26]. The removal of petroleum was determined, and then the specific degradation rate was calculated as follows:…”

Section: Petroleum Biodegradation Of Bacterial Consortia By Gravimetrmentioning

confidence: 99%

“…Klebsiella pneumoniae and P. phragmitetus isolated from petroleum-contaminated waste mud pit were known to be effective in the bioremediation process. Previous studies indicated that K. pneumoniae plays an active role in the biodegradation of petroleum [24] and P. phragmitetus is effective in the detoxification of heavy metals and PAHs [30].…”

Section: Identification Of Bacteriamentioning

confidence: 99%

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Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Özyürek

Bilkay

2020

SN Appl. Sci.

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The objectives of this study were to determine and compare the petroleum biodegradation efficiencies of three different bacterial consortia adapted to oil field. For this purpose, petroleum-contaminated waste mud sample was enriched in Bushnell Haas medium with 1% petroleum. Six different bacterial strains (four Gram-positive and two Gram-negative) were isolated from the enriched sample, and bacterial strains were identified with phenotypic and genotypic analyses. Each bacterial strain was evaluated in terms of biodegradation capacities by colorimetric analysis with the DCPIP redox indicator. Petroleum biodegradation efficiencies of three different consortia were investigated by gravimetric and GC-MS analyses. Consortium 1, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus; consortium 2, consisting of Klebsiella pneumoniae and Pannonibacter phragmitetus; consortium 3, consisting of Bacillus subtilis, Leuconostoc mesenteroides, Bacillus halotolerans, Enterococcus casseliflavus, Klebsiella pneumoniae and Pannonibacter phragmitetus, were used in petroleum biodegradation. According to the results of gravimetric analysis, biodegradation of petroleum was 48%, 77% and 82% for consortium 1, consortium 2 and consortium 3, respectively. The results of GC-MS analysis revealed that removal of long-chain n-alkanes (C 23-C 32) was in the range of 35-58% by consortium 1; 56-67% by consortium 2; and 63-74% by consortium 3. It was emphasized that consortium 2 was more effective in biodegradation of petroleum than consortium 1. This study clearly demonstrated that consortium 3 is most effective in biodegradation of petroleum. This is assumed to be due to the synergistic effect between Gram-positive and Gram-negative bacterial strains. Furthermore, consortium 3 has been found to be very successful despite the shorter incubation period compared to other consortia in the literature. In this context, it is believed that the use of consortium 3 will contribute to the bioremediation of petroleum on a large scale.

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Section: Biodegradation Of N-alkane Fractions In Petroleummentioning

confidence: 99%

“…The experiments were performed in triplicate. The method was carried out as described previously in Bilen Ozyurek and Bilkay [24].…”

Section: Petroleum Biodegradation Assaysmentioning

confidence: 99%

Section: Petroleum Biodegradation Of Bacterial Consortia By Gravimetrmentioning

confidence: 99%

Section: Identification Of Bacteriamentioning

confidence: 99%

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Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Özyürek

Bilkay

2020

SN Appl. Sci.

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“…Physical methods such as skimming, wiping, burning, water flushing, etc. are used rarely as ancient methods for dealing with the problem but are least effective (Padaki et al 2015). On the other hand, certain chemical surfactants are also used for the removal of oil, but are less acceptable as less than only 8% usable oil may be recovered (Hemmer et al 2011).…”

Section: Introductionmentioning

confidence: 99%

A newly isolated strain of Serratia sp. from an oil spillage site of Assam shows excellent bioremediation potential

et al. 2019

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A hydrocarbon-degrading strain was isolated from a petroleum oil-contaminated site which was identified on the basis of 16S rDNA gene sequencing as a member of the genus Serratia. The isolate reduced surface tension of petroleum oil supplemented medium by 48.35% with respect to control after 7 days of treatment. Fluorescence microscopy revealed that its chemotaxis was towards hydrocarbon. The isolate degraded 87.54 and 85.48% of diesel and kerosene in liquid culture, respectively, after 28 day incubation at 37 ± 2 °C. The ex situ pilot scale bioremediation experiment in which artificially contaminated soil (10 and 20% v/w kerosene) was treated for 7 days showed a germination rate of Vigna radiate seeds of 52% and 72%, respectively. Interestingly, a germination rate of 31% was obtained with the heavily contaminated soil samples collected from the oil spillage site after 20 days of bioremediation treatment. The presence of υ CH3 (asymmetric stretching), υ C=C (stretch), and υ CC (stretch) in the crude biosurfactant produced by the isolate was revealed by FTIR analysis, and emulsification index (E 24) was found 60 and 56.6%, respectively, against diesel and kerosene oil. The non-cytotoxicity nature of the biosurfactant also supports its potential application in field trial.

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Applications of Microbes in Bioremediation of Water Pollutants

Gür

Bakhshpour

Deni̇zli̇

2021

Environmental and Microbial Biotechnology

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Biodegradation of petroleum by Klebsiella pneumoniae isolated from drilling fluid

Cited by 20 publications

References 35 publications

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

Comparison of petroleum biodegradation efficiencies of three different bacterial consortia determined in petroleum-contaminated waste mud pit

A newly isolated strain of Serratia sp. from an oil spillage site of Assam shows excellent bioremediation potential

Applications of Microbes in Bioremediation of Water Pollutants

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