Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514

Varjani, Sunita; Upasani, Vivek N.

doi:10.1016/j.biortech.2016.10.006

Cited by 249 publications

(96 citation statements)

References 25 publications

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“…Recently, a P. aeruginosa NCIM 5514 isolate has been suggested as a suitable strain for bioremediation of hydrocarbon pollutants in petrochemical industry (Varjani and Upasani 2016). In this study, we show that ZS1 isolate is capable of consuming 60% crude oil at 30 °C in 14 days.…”

Section: Discussionmentioning

confidence: 99%

“…Alcanivorax borkumensis is one of the well characterized hydrocarbonoclastic marine bacterium (Akihiro et al 2003; Yakimov et al 2007) that produces glucolipids (Naether et al 2013). P. aeruginosa is also a well characterized oil-eating bacterium (Varjani and Upasani 2016) that produces rhamnolipids (Zhang and Miller 1992). Biosurfactants emulsify crude oil facilitating oil uptake and biodegradation by the oil-eating bacteria.…”

Section: Introductionmentioning

confidence: 99%

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A novel rhamnolipid-producing Pseudomonas aeruginosa ZS1 isolate derived from petroleum sludge suitable for bioremediation

Cheng

Liang

et al. 2017

AMB Expr

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Petroleum pollutants emulsified by biosurfactants facilitate uptake and biodegradation by environmental microbes. In this report, we show the characterization of an indigenous surfactant-producing crude-oil-eating microbe isolated from petroleum-sludge in Zhoushan islands, China, where one of the national strategic petroleum reservoirs is located. We examined biosurfactant activities using surface tension analysis on mixed culture originated from oil-sludge microbes. In parallel, dynamics of individual microbial populations in cultures were monitored using the terminal fluorescence labeled (TFL)-RFLP method. Biosurfactant activity was found to be associated with a dominant microbial species designated as ZS1 (Zhou-Shan isolate 1). Cell-free supernatant from the ZS1 culture exhibited 100% emulsification index against crude oil and reduces surface tension to 26.5 mN/m. Sequence-based analysis suggested that the ZS1 belongs to the group of Pseudomonas aeruginosa. LC–MS/MS analysis indicated that the rhamnolipids produced by the ZS1 consisted of 7 monorhamnolipid and 11 dirhamnolipid homologues (RL7-11), two of which were novel. Maximum yield of rhamnolipids in shake-flask ZS1 culture could reach 44 g/l. Furthermore, we showed that ZS1 was capable of growing in MS medium supplemented with 1% crude oil as sole carbon source, in which cell mass increase coincided with the formation of crude-oil emulsion. Half of the crude oil was consumed by the ZS1 in 12 days. Taken together, our results show that ZS1 produces high level of rhamnolipids that effectively emulsify crude oil accelerating its uptake and degradation. Hence, it is suitable for bioremediation of petroleum pollutants in Zhoushan coastal areas.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

A novel rhamnolipid-producing Pseudomonas aeruginosa ZS1 isolate derived from petroleum sludge suitable for bioremediation

Cheng

Liang

et al. 2017

AMB Expr

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“…During the microbial remediation process, PH components are absorbed, degraded and transformed in the contaminated system. At the same time, PH can be used as a carbon source for the survival and reproduction of microorganisms and finally converted into non‐polluting substances such as carbon dioxide, methane and water . In recent years, several groups have screened and isolated thousands of promising PH‐degrading microorganisms from the natural environment .…”

Section: Introductionmentioning

confidence: 99%

Microbial community dynamics and functional responses that contribute to tolerance of high concentrations of petroleum hydrocarbon

Cui

Liu²,

et al. 2020

J of Chemical Tech & Biotech

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BACKGROUND It is a common problem that high‐concentration petroleum hydrocarbon (hc‐PH) is difficult to degrade and remove using biological methods. RESULTS In this study, when the concentrations of petroleum hydrocarbon (PH) were increased from 972.4 ± 40.3 to 6953.8 ± 294.3 mg L−1, all degradation rates of PH by activated sludge (AS) were beyond 79%. However, the degradation rate of hc‐PH (from 9958.3 ± 401.6 to 19 953.3 ± 896.5 mg L−1) by AS was significantly lower than that of low‐concentration petroleum hydrocarbon (lc‐PH) (from 972.4 ± 40.3 to 6953.8 ± 294.3 mg L−1). Meanwhile, the heterotrophic microorganism number and bacterial community diversity in the hc‐PH environment were lower than those in the lc‐PH environment. Sequencing results of the bacterial community showed that the dominant bacterial community of AS was stable and composed by the genera Pseudomonas, Acinetobacter and Burkholderia‐Paraburkholderia in the hc‐PH condition. Moreover, aimed to enhance the tolerance to hc‐PH stress, the relative abundances of microbial community functions of environmental adaptation, membrane transport, replication and repair were increased. However, the digestive system, circulatory system and endocrine system of the microbial community were significantly disturbed by hc‐PH, which eventually caused the degradation rate of PH by AS to decrease significantly. CONCLUSION Thus the decrease of microbial community diversity and tolerance ability can be regarded as the main limiting factors to enhance the degradation rate of hc‐PH by microorganisms. © 2019 Society of Chemical Industry

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“…The soil rhizosphere helps in structural rearrangement of hydrocarbons arriving from several sources (Varjani and Upasani, 2016). As soon as go in to the soil, the multifaceted compounds of PHs can detach into distinct amalgams dependent on their physicochemical possessions.…”

Section: Fate Of Phs In Soil Environmentmentioning

confidence: 99%

“…It is suggested that redox potential increases the degradation of PHs by expanding their bioavailability thus increasing microbial metabolism (Varjani and Upasani, 2016).…”

Section: Redox Potentialmentioning

confidence: 99%

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

Fatima¹,

Imran²,

Naveed³

et al. 2017

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Biodegradation of petroleum hydrocarbons by oleophilic strain of Pseudomonas aeruginosa NCIM 5514

Cited by 249 publications

References 25 publications

A novel rhamnolipid-producing Pseudomonas aeruginosa ZS1 isolate derived from petroleum sludge suitable for bioremediation

A novel rhamnolipid-producing Pseudomonas aeruginosa ZS1 isolate derived from petroleum sludge suitable for bioremediation

Microbial community dynamics and functional responses that contribute to tolerance of high concentrations of petroleum hydrocarbon

Plant-bacteria synergism: An innovative approach for the remediation of crude oil-contaminated soils

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