Biodegradation of Phenanthrene Polluted Soil through Native Strains in the Darkhouvin Oil Field

Abbasi, Maryam; Kafilzadeh, Farshid; Sabokbar, A; Haddadi, Azam

doi:10.1080/10406638.2022.2097272

Cited by 2 publications

(1 citation statement)

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“…There are several reports of microbial bioremediation of various PAHs. Most of these studies include degradation of single PAHs by using one bacterium (Meena et al 2016;Li et al 2021;Abbasi et al 2022;Imam et al 2023). However, PAHcontamination in environment frequently occurs as combination of various PAHs making their bioremediation more di cult (Agrawal et al 2019).…”

Section: Introductionmentioning

confidence: 99%

Pseudomonas aeruginosa PR23 isolated from oil contaminated soil tolerate and degrades mixture of polyaromatic hydrocarbons and express novel proteins

Jindal,

Aggarwal

2023

Preprint

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Pseudomonas aeruginosa PR23 isolated from the hydrocarbon contaminated soil tolerate and degrades mixture of polyaromatic hydrocarbons (PAHs) having initial concentration of 1300 ppm. The degradation and intermediates formed were assessed through GC-MS analysis. The isolated strain was able to degrade 59.2% mixture of PAHs in 3 days and 71.6% by day 15. Effect of PAHs on protein expression in Pseudomonas aeruginosa PR23 was studied through nano LC-MS/MS. Thirty-six proteins were observed that showed more than 2-fold increased expression in the presence of mixture of PAHs. Out of these proteins, 7 proteins are reported for their role in degradation of naphthalene, phenanthrene, and pyrene. Data also showed the presence of 16 proteins that are uniquely expressed in the presence of mixture of PAHs. A Twin-arginine translocation signal peptide (tat system) known for transportation of folded proteins across the cell membrane showed more than 8-fold increased expression in the presence of mixture of PAHs. Results indicate that isolated strain adopts the conditions in the presence of mixture of PAHs by modulating its metabolic and physiological processes. These findings suggest that Pseudomonas aeruginosa PR23 may be a suitable candidate to be used for the development of strategies for bioremediation of mixture of PAHs.

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