Biodegradation of reactive dye (Verofix Red) by the white-rot fungus Phanerochaete chrysosporium using Box-Behnken experimental design

Abstract

Biodegradation of reactive dye using the white rot fungus Phanerochaete chrysosporium was demonstrated by the disappearance of the colour of reactive dye. An increase in dye concentration showed decreased dye degradation, and maximum dye degradation and increased biomass was observed in medium amended with limited source of nitrogen. Increasing days showed increased biomass production as well as dye degradation. BoxBehnken design with three variables like dye concentration (200, 700 and 1200 mg/l), days (2, 6 and 10) and nitrogen concentration at three different levels (0.054, 0.081 and 0.108 g/l) were studied to identify a signi®cant correlation between the effect of these variables on the amount of biodegradation of reactive dye. The methodology identi®es the principal experimental variables, which have the greatest effect in the biodegradation process. The experimental values are in good agreement with predicted values, the correlation coef®cient being 0.9988.

Decolourisation of Reactive Black 5 by Funalia trogii immobilised on Luffa cylindrica sponge

“…In this study, about 100-200 mg/l carbohydrate concentrations were detected as total carbohydrate after sterilisation of the sponge and this concentration was found to be sufficient for decolourisation. Limited and sufficient level nitrogen required for decolourisation [40,[46][47][48]. A nitrogen source was present in the structure of the dye and in the sponge and F. trogii was able to degrade these natural compounds by extracellular enzymes when it required.…”