Biodegradation of Spilled-oil by Immobilization Microorganisms on Diatomite-based Carrier Material

Abstract

Abstract. This paper focused on research the dynamic adsorption capacity of immobilized microorganisms on diatomite-based carrier material. Several important parameters were studied, including different immobilization methods, pH value of cross linking agent, the influence on the physical properties and degradation effect of immobilized microspheres with various immobilization bacteria amount. The experimental results showed that direct immobilization method was better that of the other methods. After crosslinking at the temperature of 25 ˚C for 24 h, the best immobilization conditions were at pH 5-6 in the cross-linking agent solution. The volume ratio of immobilization bacteria liquid and gel solution was 1:5. The immobilization of inorganic salts could accelerate the recovery of the activity of immobilized microorganisms, and the surface structure of the microspheres was observed before and after degradation by SEM. The highest degradation rate could reach 40% for free M4, while 70% for immobilized M4, which increased oil degradation rate over 30%.

Adsorptive bioremediation of soil highly contaminated with crude oil

“…There was a biopreparation obtained from hydrocarbon-degrading bacteria Herbaspirillum chlorophenolicum strain FA1 immobilized on an alginate-diatomite matrix. This preparation is characterized by a high abundance of the microorganisms and a high crude oil degradation rate in soil compared to free-living cells or immobilized on alginate (Hu et al, 2011;Wang et al, 2012Wang et al, , 2015. Microbial inocula with nutrients immobilized on diatomaceous earth constitute an effective formulation to remediate petroleum-contaminated soils with a high concentration of hydrocarbons.…”