Biodegradation of Terephthalic Acid by Rhodococcus biphenylivorans Isolated from Soil

Terephthalic acid (TPA) is an isomer of ortho-phthalic acid, which is widely used in the chemical industry to produce artificial fibers and plastics, including polyethylene terephthalate; it is a widespread environmental pollutant. The ability of two strains of Glutamicibacter spp. PB8-1 (=ВКМ Ac-2934D) and BO25 (=ВКМ Ac2935D) isolated from the salt mining area (Perm krai, Russia) to grow using terephthalic acid as the only source of carbon and energy was studied. The strains PB8-1 and BO25 could utilize high concentrations of TPA (30 g/L), which was shown for TPA-degrading bacteria for the first time. Strains PB8-1 and BO25 were halotolerant bacteria: they grew in the NaCl-free medium or at NaCl concentrations of up to 90 g/L in a rich medium and up to 60 g/L in a mineral medium supplemented with TPA. No bacteria capable of degrading TPA under saline conditions were previously described. The growth of the strain BO25 using TPA was accompanied by the accumulation and subsequent degradation of protocatechuic acid (PCA), suggesting that the TPA metabolism occurred through PCA, which was previously described for bacteria of different taxa, including actinobacteria. Thus, TPA-degrading strains Glutamicibacter spp. PB8-1 and BO25 are promising for the development of bioremediation methods for saline soils and wastewater contaminated with TPA.

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“…The strain Rhodococcus sp. N2, which utilizes 10 g/L of TPA within 5 days has been described [30]. The studied strains Glutamicibacter spp.…”

Section: Resultsmentioning

confidence: 99%

Halotolerant Terephthalic Acid-Degrading Bacteria of the Genus Glutamicibacter

Yastrebova

Malysheva²,

Плотникова³

2022

Appl Biochem Microbiol

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“…C4B showed complete degradation of terephthalate after only 19 hr of incubation at 50 °C (Roberts et al, 2020 ). Terephthalate-degrading organisms are widespread and have been isolated from numerous sources, including soil (Suwanawat et al, 2019 ), household compost (Sulaiman et al, 2012 ), and wastewater (Kimura & Ito, 2001 ). Examples of terephthalate-degrading isolates include Alcanivorax (Delacuvellerie et al, 2019 ), Syntrophus (Morris et al, 2013 ) genera within the Proteobacteria; Pelotomaculum (Morris et al, 2013 ) genus within the Firmicutes; and Rhodococcus (Suwanawat et al, 2019 ) genus within the Actinobacteria.…”

Section: Introductionmentioning

confidence: 99%

“…Terephthalate-degrading organisms are widespread and have been isolated from numerous sources, including soil (Suwanawat et al, 2019 ), household compost (Sulaiman et al, 2012 ), and wastewater (Kimura & Ito, 2001 ). Examples of terephthalate-degrading isolates include Alcanivorax (Delacuvellerie et al, 2019 ), Syntrophus (Morris et al, 2013 ) genera within the Proteobacteria; Pelotomaculum (Morris et al, 2013 ) genus within the Firmicutes; and Rhodococcus (Suwanawat et al, 2019 ) genus within the Actinobacteria. Chemical depolymerization of PET offers a faster alternative to naturally occurring PETases by chemically deconstructing PET polymers into smaller molecules, which are more easily biodegradable (Müller et al, 2001 ; Paliwal & Mungray, 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Schaerer

Wood

Aloba

et al. 2023

Journal of Industrial Microbiology and Biotechnology

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Most plastic waste accumulates in landfills or the environment. Natural microbial metabolisms can degrade plastic polymers. Unfortunately, biodegradation of plastics is slow even under ideal conditions; depolymerization of plastic is the rate limiting step. Rapid chemical depolymerization yields biodegradable plastic monomers, improving biodegradation rates. Here we demonstrate that ammonium hydroxide depolymerizes PET into terephthalic acid and terephthalic acid monoamide which are rapidly metabolized by diverse consortia obtained from compost and sediment. By neutralizing the product with phosphoric acid prior to bioprocessing, the final product contains plastic-derived carbon and biologically accessible nitrogen and phosphorus from the process reactants, removing the need for culture medium. Three microbial consortia were able to degrade chemically deconstructed PET in ultrapure water and scavenged river water without the addition of nutrients, with no statistically significant difference in growth rate compared to communities grown on deconstructed PET in Bushnell Haas minimal culture medium. The consortia were dominated by Rhodococcus spp., Hydrogenophaga spp., and many lower abundance genera. All taxa were related to species known to degrade aromatic compounds. Microbial consortia are known to confer flexibility in processing diverse substrates. To highlight the versatility of these consortia, we also demonstrate that two microbial consortia can grow on similarly deconstructed polyesters, polyamides, and polyurethanes in water instead of medium. Our findings suggest that using microbial communities enable flexible bioprocessing of mixed plastic wastes. We also demonstrate the flexibility of this approach for coupled chemical deconstruction and bioprocessing.

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“…Arthrobacter sp. 0574, Pseudomonas sp., Bacillus sp., Comamonas testosterone and Rhodococcus biphenylivorans have studied on biodegradation of TA and effectiveness results have achieved [10,21,22,23,24,25,26,27]. Recent studies suggest that applications of molecular techniques using real-time PCR to environmental samples have proven to the practicable.…”

Section: Introductionmentioning

confidence: 99%

Biodegradation of Terephthalic Acid by Isolated Active Sludge Microorganisms and Monitoring of Bacteria in a Continuous Stirred Tank Reactor

Aksu

Vural

Karabey

et al. 2021

Braz. arch. biol. technol.

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Terephthalic acid is extensively used as an important raw material in polyester fibers, as well as the production of polyethylene terephthalate bottles and textile industries. Especially, in the petrochemical industry, toxic chemicals are released to the atmosphere during the production of polyethylene terephthalate, unless the wastewater treatment is carried out. It's a well-known fact that chemicals have serious side effects on human health, so manufacturing companies should not dispose of such harmful chemicals without treatment. Biodegradation is an effective option for eco-friendly degradation of hydrocarbons. Hydrocarbondegrading bacteria are everywhere in environment and can utilize these chemicals as sources of carbon and energy. In the present study, aerobic bacterial strains T1, T4, T5, and TK were isolated from activated sludge and crude oil deposits of a petrochemical company in Turkey. The strains were identified to be Pseudomonas sp., Chryseobacterium sp., Burkholderia sp., and Arthrobacter sp. according to morphological, physiological and biochemical characteristics. The strains were able to degrade about 100% of 100 mg/L terephthalic acid within, respectively, 8, 67, 52, 24 hour as sole carbon and energy source. Therefore, these isolates can be HIGHLIGHTS  Terephthalic acid one of the phthalates has been known endocrine disrupting chemical and degraded by bacterial strains. Four bacterial isolates identified as Arthrobacter sp., Chryseobacterium sp. Burkholderia sp. and Pseudomonas sp are able to degrade 100 mgL -1 concentration terephthalic acid. In order to monitory bacteria in CSTR reactor, Real-time PCR was used. Aksu, D., et al.

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Biodegradation of Terephthalic Acid by Rhodococcus biphenylivorans Isolated from Soil

Cited by 8 publications

References 11 publications

Halotolerant Terephthalic Acid-Degrading Bacteria of the Genus Glutamicibacter

Halotolerant Terephthalic Acid-Degrading Bacteria of the Genus Glutamicibacter

Versatile microbial communities rapidly assimilate ammonium hydroxide-treated plastic waste

Biodegradation of Terephthalic Acid by Isolated Active Sludge Microorganisms and Monitoring of Bacteria in a Continuous Stirred Tank Reactor

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