Biodegradation of Tetrahydrofuran and 1,4-Dioxane by Soluble Diiron Monooxygenase in &lt;b&gt;&lt;i&gt;Pseudonocardia&lt;/i&gt;&lt;/b&gt; sp. Strain ENV478

Masuda, Hisako; McClay, Kevin; Steffan, Robert J.; Zylstra, Gerben J.

doi:10.1159/000343817

Cited by 50 publications

(38 citation statements)

References 30 publications

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“…S1 in the supplemental material. Monooxygenase homologs in CB1190 were selected from this list based on previous laboratory studies demonstrating the ability of bacteria harboring these enzymes to transform dioxane or other similarly recalcitrant compounds (19,20,28). We evaluated DXMO, PHE, PrMO, alcohol dehydrogenase (ADH), and aldehyde dehydrogenase (ALDH) as potential gene targets that could specifically indicate dioxane degradation in environmental samples.…”

Section: Resultsmentioning

confidence: 99%

“…DXMO and ALDH are homologs of the tetrahydrofuran oxygenase beta subunit (thmB) and the aldehyde dehydrogenase (thmH) of Pseudonocardia tetrahydrofuranoxydans strain K1, respectively (15,47). These targets have been shown to be involved in the metabolic and cometabolic biodegradation of tetrahydrofuran and dioxane (19,29). Grostern and coworkers (29) identified these genes as being differentially upregulated in CB1190 during growth on dioxane compared to pyruvate/glyoxylate, which is supported by our time course char- acterization of these genes in CB1190.…”

Section: Discussionmentioning

confidence: 99%

“…1,4-Dioxane (hereafter "dioxane") is a water contaminant of emerging concern with the potential for transformation by a selected group of microorganisms (16)(17)(18)(19)(20). Dioxane is classified as a likely human carcinogen (21) and has been detected at several hazardous waste sites (22) due to its widespread use as a stabilizer for chlorinated solvents (mainly 1,1,1-trichloroethane) and improper storage and disposal of dioxane-containing solvents (23).…”

mentioning

confidence: 99%

“…Metabolic degradation is less common, with only a few microorganisms being able to use dioxane as the sole source for carbon and energy (18,(30)(31)(32). Cometabolic pathways include several monooxygenase enzymes required for dioxane degradation, including the methane (MMO), propane (PrMO), phenol (PHE), tetrahydrofuran (THFMO), and toluene (TOL, T4MO, or RMO), monooxygenases (19,20,28,33,34). THFMO was proven to catalyze THF as well as dioxane biodegradation in Pseudonocardia sp.…”

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confidence: 99%

“…THFMO was proven to catalyze THF as well as dioxane biodegradation in Pseudonocardia sp. strain ENV478 (19). In addition, the recently completed genomic assembly and annotation of Pseudonocardia dioxanivorans CB1190 (hereafter "CB1190") (35), a bacterium able to use dioxane as the sole source of carbon and energy (18,32), facilitated a comprehensive in silico analysis of genes encoding several multicomponent monooxygenase clusters (35), including a plasmidencoded region containing the entire THF degradation gene cluster from Pseudonocardia sp.…”

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confidence: 99%

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Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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Bacterial multicomponent monooxygenase gene targets in Pseudonocardia dioxanivorans CB1190 were evaluated for their use as biomarkers to identify the potential for 1,4-dioxane biodegradation in pure cultures and environmental samples. Our studies using laboratory pure cultures and industrial activated sludge samples suggest that the presence of genes associated with dioxane monooxygenase, propane monooxygenase, alcohol dehydrogenase, and aldehyde dehydrogenase are promising indicators of 1,4-dioxane biotransformation; however, gene abundance was insufficient to predict actual biodegradation. A time course gene expression analysis of dioxane and propane monooxygenases in Pseudonocardia dioxanivorans CB1190 and mixed communities in wastewater samples revealed important associations with the rates of 1,4-dioxane removal. In addition, transcripts of alcohol dehydrogenase and aldehyde dehydrogenase genes were upregulated during biodegradation, although only the aldehyde dehydrogenase was significantly correlated with 1,4-dioxane concentrations. Expression of the propane monooxygenase demonstrated a time-dependent relationship with 1,4-dioxane biodegradation in P. dioxanivorans CB1190, with increased expression occurring after over 50% of the 1,4-dioxane had been removed. While the fraction of P. dioxanivorans CB1190-like bacteria among the total bacterial population significantly increased with decrease in 1,4-dioxane concentrations in wastewater treatment samples undergoing active biodegradation, the abundance and expression of monooxygenase-based biomarkers were better predictors of 1,4-dioxane degradation than taxonomic 16S rRNA genes. This study illustrates that specific bacterial monooxygenase and dehydrogenase gene targets together can serve as effective biomarkers for 1,4-dioxane biodegradation in the environment.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

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confidence: 99%

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Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Gedalanga

Pornwongthong

Mora³

et al. 2014

Appl Environ Microbiol

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1,4‐Dioxane Treatment Technologies

DiGuiseppi

Walecka-Hutchison

Hatton³

2016

Remediation Journal

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A Multiple Lines of Evidence Framework to Evaluate Intrinsic Biodegradation of 1,4‐Dioxane

Gedalanga

Madison

Yu³

et al. 2016

Remediation Journal

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Development of a multiple lines of evidence (MLOE) framework to evaluate the intrinsic biodegradation potential of 1,4‐dioxane is vital to implementing management strategies at groundwater sites impacted by 1,4‐dioxane. A comprehensive MLOE approach was formed to provide significant evidence of natural degradation of 1,4‐dioxane comingled with tetrahydrofuran (THF) within a large, diffuse plume. State‐of‐the art molecular biological analyses and compound‐specific isotope analysis (CSIA) were employed to support more traditional approaches for data analysis (concentration trend analyses, spatial distribution, temporal changes, geochemical biodegradation attenuation indicators, plume mass estimates, and fate and transport modeling). The molecular analyses demonstrated that microorganisms capable of both metabolic and cometabolic degradation of 1,4‐dioxane were present throughout the groundwater plume, whereas the CSIA data provided supporting evidence of biodegradation. 1,4‐Dioxane biomarkers were present and abundant throughout the 1,4‐dioxane plume, and our biomarkers tracked the plume with reasonable accuracy. Evidence also suggests that THF‐driven cometabolic biodegradation as well as catabolic 1,4‐dioxane biodegradation were active at this site. These data supplemented the traditional lines of evidence approaches, which demonstrated that 1,4‐dioxane attenuation was occurring across the groundwater plume and that nondestructive physical processes alone did not account for the observed 1,4‐dioxane attenuation. This MLOE framework combining new and traditional analyses demonstrates that this site has a significant capacity for intrinsic biodegradation of 1,4‐dioxane. ©2016 Wiley Periodicals, Inc.

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Biodegradation of Tetrahydrofuran and 1,4-Dioxane by Soluble Diiron Monooxygenase in <b><i>Pseudonocardia</i></b> sp. Strain ENV478

Cited by 50 publications

References 30 publications

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

Identification of Biomarker Genes To Predict Biodegradation of 1,4-Dioxane

1,4‐Dioxane Treatment Technologies

A Multiple Lines of Evidence Framework to Evaluate Intrinsic Biodegradation of 1,4‐Dioxane

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