Biodegradation of Total Petroleum Hydrocarbons in Soil: Isolation and Characterization of Bacterial Strains from Oil Contaminated Soil

Abstract

In this study, we isolated seven strains (termed BY1–7) from polluted soil at an oil station and evaluated their abilities to degrade total petroleum hydrocarbons (TPHs). Following 16 rRNA sequence analysis, the strains were identified as belonging to the genera Bacillus, Acinetobacter, Sphingobium, Rhodococcus, and Pseudomonas, respectively. Growth characterization studies indicated that the optimal growth conditions for the majority of the strains was at 30 °C, with a pH value of approximately 7. Under these conditions, the strains showed a high TPH removal efficiency (50%) after incubation in beef extract peptone medium for seven days. Additionally, we investigated the effect of different growth media on growth impact factors that could potentially affect the strains’ biodegradation rates. Our results suggest a potential application for these strains to facilitate the biodegradation of TPH-contaminated soil.

Promising Strains of Hydrocarbon-Oxidizing Pseudomonads with Herbicide Resistance and Plant Growth-Stimulating Properties for Bioremediation of Oil-Contaminated Agricultural Soils

“…P. alcaligenes UOM 10 and P. frederiksbergensis UOM 11 proved to be the most effective. They are representatives of species that are well-known for their ability to degrade oil, petroleum products, and polycyclic aromatic hydrocarbons (PAHs) [72][73][74][75][76][77]. An additional advantage of the isolated bacteria is their ability to synthesize lipase (Table 3).…”

Biodegradation of Phenanthrene by Alcaligenes ammonioxydans, (VITRPS2) strain isolated from petroleum contaminated soil: Degradation, Inhibition kinetics and metabolic pathway

“…Within 7 days, strain destroyed more than 72% of the given phenanthrene. Most petroleum hydrocarbon polluted locations have high levels of A. ammonioxydans, indicating their capacity to metabolise complex compounds (Roy et al, 2018;Wang et al, 2020).…”

Microbial Resources, Fermentation and Reduction of Negative Externalities in Food Systems: Patterns toward Sustainability and Resilience

“…[38,39] Beneficial plant-microbe interactions to breed 'microbe-optimized plants' [32] Microalgae and new application in food, feed, and nutraceuticals chains [40,41] Microbial-based tailored solutions for sustainable feeding regimen [42] As reported in the scientific literature, it is possible to find so many examples as to suggest a potential systemic application of microbes as mitigating agents in the primary production. In several cases, the target is the 'remediation' of negative trends: microorganisms selected to reduce carbon dioxide [19,22], bioconversion of pollutants in water via microbial [19,26,27], microbial-driven bioremediation of soil [28,29], and microbial-based decomposition of endocrine disruptors from trophic chains [22]. In addition, we can find 'green' microbial alternatives to standard solutions, such as substitute to antibiotic [35][36][37], pesticides [32,34], fertilizers/stimulants [32,33], feeding regimen [42], nitrogen sources [19], protein production [19,25], and to make water potable [30,31].…”