Biodegradation of Tributyltin (TBT) by Extremophile Bacteria from Atacama Desert and Speciation of Tin By-products

Yáñez, Jorge; Riffo, Paula; Santander, Paola; Mansilla, Héctor D.; Mondaca, M. A.; Campos, Víctor; Amarasiriwardena, Dulasiri

doi:10.1007/s00128-015-1561-1

Cited by 12 publications

(5 citation statements)

References 30 publications

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“…were able to use TBT as the sole carbon source. This result is not surprising as Pseudomonas members have often been reported as TBT-resistant and as TBT-degraders (Roy et al 2004 ; Khanolkar et al 2015 ; Yáñez et al 2015 ; Ebah et al 2016 ). In addition they are also known to degrade a wide range of other sediment contaminants (Wasi et al 2013 ).…”

Section: Discussionmentioning

confidence: 87%

iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment

Polrot

Kirby

Olorunniji

et al. 2022

World J Microbiol Biotechnol

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Standard methods of microbial cultivation only enable the isolation of a fraction of the total environmental bacteria. Numerous techniques have been developed to increase the success of isolation and cultivation in the laboratory, some of which derive from diffusion chambers. In a diffusion chamber, environmental bacteria in agar medium are put back in the environment to grow as close to their natural conditions as possible, only separated from the environment by semi-permeable membranes. In this study, the iChip, a device that possesses hundreds of mini diffusion chambers, was used to isolate tributyltin (TBT) resistant and degrading bacteria. IChip was shown to be efficient at increasing the number of cultivable bacteria compared to standard methods. TBT-resistant strains belonging to Oceanisphaera sp., Pseudomonas sp., Bacillus sp. and Shewanella sp. were identified from Liverpool Dock sediment. Among the isolates in the present study, only members of Pseudomonas sp. were able to use TBT as a sole carbon source. It is the first time that members of the genus Oceanisphaera have been shown to be TBT-resistant. Although iChip has been used in the search for molecules of biomedical interest here we demonstrate its promising application in bioremediation.

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Section: Discussionmentioning

confidence: 87%

iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment

Polrot

Kirby

Olorunniji

et al. 2022

World J Microbiol Biotechnol

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“…In contrast to TBT, a compound biodegraded by bacteria (e.g., Moraxella osloensis (Yáñez et al 2015), Pseudomonas sp. (Bernat et al 2014), and Enterobacter cloacae (Sakultantimetha et al 2011)), fungi (e.g., C. elegans (Bernat and Długoński 2006), Cunninghamella echinulata (Soboń et al 2016), and Coniophora puteana (White et al 1999)), the alga Chlorella vulgaris (Tsang et al 1999), and crab Thalamita crenata (Chen et al 2016), DBT and MBT have been described as being degraded by only a few microbial strains.…”

Section: Resultsmentioning

confidence: 99%

Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Siewiera

Różalska

Bernat

2017

Environ Sci Pollut Res

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Dibutyltin (DBT) is an environmental pollutant characterized by immunotoxic, neurotoxic, and pro-oxidant properties. In this study, an attempt was made to enhance DBT elimination by the Metarhizium robertsii strain. We observed enhanced fungal growth in the bioreactor (pO2 ≥ 20%) compared to flask cultures (μ max increased from 0.061 to 0.086 h−1). Moreover, under aerated conditions, M. robertsii mycelium with “hairy” morphology biodegraded DBT (20 mg l−1) 10-fold faster in the bioreactor than in the flask cultures. Monobutyltin (MBT) and a hydroxylated derivative of MBT (OHBuSnH2) were detected as by-products of dibutyltin debutylation. Simultaneous usage of glucose and butyltins indicates the comatabolic nature of monobutyltin and dibutyltin removal. In order to protect fungal cells from oxidative stress caused by DBT presence, vitamin C (20 mg l−1) was applied. Supplementation with ascorbic acid (AA) resulted in a 3-fold acceleration of MBT removal during the first 7 h of incubation. Using the HPLC-MS/MS technique, a quantitative analysis of malondialdehyde (MDA), a marker of oxidative stress, was performed. In the AA presence, a decrease in the MDA amount (about 45%) was observed compared to the case with fungal cells exposed to DBT alone.Electronic supplementary materialThe online version of this article (doi:10.1007/s11356-017-8764-4) contains supplementary material, which is available to authorized users.

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“…Наблюдаемое возрастание численности бактерий класса Gammaproteobacteria в процессе трансформации ТБО может быть связано с их повышенной устойчивостью к ООС. Многие виды Gammaproteobacteria (Aeromonas molluscorum, Klebsiella pneumonia, Moraxella osloensis, Pseudomonas sp., Shewanella putrefaciens, Stenotrophomonas chelatiphaga) известны как эффективные деструкторы соединений бутилолова и отличаются высокой толерантностью к токсикантам Yáñez et al, 2015).…”

Section: таксономическая структура прокариотных сообществunclassified

Biotransformation of butyltin compounds and microbiome diversity in the bottom sediments of the Gulf of Finland, the Baltic Sea

Кузикова,

Жаковская,

Медведева

2024

Ecosystem Transformation

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Загрязнение морской среды оловоорганическими соединениями представляет се- рьезную экологическую проблему ввиду их чрезвычайной токсичности для широкого круга орга- низмов. В донных осадках Копорской губы Финского залива выявлен высокий уровень загрязнения соединениями бутилолова. Концентрация трибутилолова и дибутилолова составила 35.7 нг(Sn)/г а.с.о. и 9.7 нг(Sn)/г а.с.о. соответственно. Установлено, что трансформация соединений бутило- лова в донных осадках происходит за счет автохтонной микробиоты осадков. Скорость убыли трибутилолова и дибутилолова составила 0.014 сут-1 и 0.022 сут-1, а периоды их полураспада 49.5 и 31.5 суток соответственно. Метагеномный анализ выявил изменения в таксономическом соста- ве и уменьшение видового разнообразия микробиомов донных осадков в процессе трансфор- мации оловоорганических соединений. В ходе биодеградации соединений бутилолова в донных осадках возрастает численность бактерий родов Acidithiobacillus, Halothiobacillus, Alicyclobacillus, Sulfurospirillum, Denitrovibrio, а также метаногенные археи рода Methanolobus, что может свиде- тельствовать об их потенциальном участии в биодеградации оловоорганических соединений. Полученные результаты могут быть использованы для выявления видов-активных деструкторов трибутилолова с целью разработки научно-обоснованных методов деконтаминации донных осад- ков, загрязненных оловоорганическими соединениями. Pollution of the marine environment with organotin compounds is a serious environmental problem due to their extreme toxicity to a wide range of organisms. A high level of contamination with butytin compounds was detected in the bottom sediments of the Koporye Bay, the Gulf of Finland, the Baltic Sea. The concentration of tributyltin and dibutyltin reached up to 35.7 ng(Sn)/g DW and 9.7 ng(Sn)/g DW, respectively. The butyltin compounds transformed in bottom sediments due to the activity of autochthonous sediment microbiota. The degradation rate of tributyltin and dibutyltin was 0.014 day-1 and 0.022 day-1, their half-life, 49.5 and 31.5 days, respectively. Metagenomic analysis revealed the changes in taxonomic composition and the decrease in species diversity of the bottom sediment microbiomes during the transformation of organotin compounds. During the biodegradation of butyltin compounds in bottom sediments, the abundance of bacteria of the genera Acidithiobacillus, Halothiobacillus, Alicyclobacillus, Sulfurospirillum, Denitrovibrio, as well as of methanogenic archaea of the genus Methanolobus increases, which may indicate their potential participation in the biodegradation of organotin compounds. These results may be used to identify active tributyltin degraders for developing self-purification methods of bottom sediments contaminated with organotin compounds.

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Biodegradation of Tributyltin (TBT) by Extremophile Bacteria from Atacama Desert and Speciation of Tin By-products

Cited by 12 publications

References 30 publications

iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment

iChip increases the success of cultivation of TBT-resistant and TBT-degrading bacteria from estuarine sediment

Efficient dibutyltin (DBT) elimination by the microscopic fungus Metarhizium robertsii under conditions of intensive aeration and ascorbic acid supplementation

Biotransformation of butyltin compounds and microbiome diversity in the bottom sediments of the Gulf of Finland, the Baltic Sea

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