Biodegradation of Trichloroethylene and Dichloromethane in Contaminated Soil and Groundwater

DeWeerd, Kim A.; Flanagan, William P.; Brennan, Michael J.; Principe, Jan M.; Spivack, James L.

doi:10.1080/10889869891214196

Cited by 18 publications

(14 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Understanding the redox state along Little Bayou Creek is important because Tc can be reduced from TcO 4 À , the dominant form in near-surface waters (Wildung et al, 1979), to Tc(V) or Tc(IV) precipitates by metal-and sulfate-reducing bacteria (Lloyd and Macaskie, 1996;Lloyd et al, 1998;Lloyd et al, 2000;and Wildung et al, 2000). Moreover, TCE can be biodegraded to cis-1,2-dichloroethene (cDCE) under sulfate-reducing conditions (DeWeerd et al, 1998). Propane was used to simulate TCE volatilization.…”

Section: Background and Methodsmentioning

confidence: 98%

Using Tracer Tests to Assess Natural Attenuation of Contaminants along a Channelized Coastal Plain Stream

Mukherjee

Fryar

LaSage

2005

Environmental and Engineering Geoscience

View full text Add to dashboard Cite

Tracer tests have been widely used in studies of solute transport, gas exchange, and nutrient cycling in streams. However, the use of tracer tests to assess natural attenuation of ground-water-derived contaminants in streams, particularly from point sources, has been limited. We used tracer tests in conjunction with stream gauging and contaminant analyses to study the fate of trichloroethene (TCE) and technetium-99 ( 99 Tc), which seep from industrial contaminant plumes into a channelized, first-order stream in the Coastal Plain of western Kentucky. Six tests were conducted over a 20-month period along a 300-m reach downstream of contaminated springs. Bromide, rhodamine WT, and nitrate were introduced as slug tracers to assess dilution, sorption, and reduction, respectively. Propane was added as a continuous, volatile tracer. Tracer transport was modeled as onedimensional, with transient storage and first-order mass loss. Results indicate that (1) TCE is attenuated by volatilization; (2) TCE sorption, TCE reduction, and 99 Tc reduction are negligible or absent; and (3) dilution is negligible along the study reach.

show abstract

Section: Background and Methodsmentioning

confidence: 98%

Using Tracer Tests to Assess Natural Attenuation of Contaminants along a Channelized Coastal Plain Stream

Mukherjee

Fryar

LaSage

2005

Environmental and Engineering Geoscience

View full text Add to dashboard Cite

show abstract

“…strain PCE1) (Gerritse et al, 1999;Holliger et al, 1999). Much like various other dehalorespiring bacteria (for example Desulfomonile tiedjei, Desulfuromonas chloroethenica, Dehalobacter restrictus, Dehalospirillum multivorans, Dehalococcoides ethenogenes) (Holliger et al, 1999), the Desulfitobacteria have attracted a great deal of interest over the past few years as they have considerable potential for the degradation of polyhalogenated pollutants such as tetrachloroethene (PCE), trichloroethene (TCE), carbon tetrachloride, and pentachlorophenol (PCP) in soils and groundwater (Meyer et al, 1993;Dolfing and Beurskens, 1995;Bouchard et al, 1996;Lee et al, 1997;Spuij et al, 1997;DeWeerd et al, 1998;Gerritse et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

Coexistence of a sulphate‐reducing Desulfovibrio species and the dehalorespiring Desulfitobacterium frappieri TCE1 in defined chemostat cultures grown with various combinations of sulphate and tetrachloroethene

Drzyzga

Gerritse²,

Hoogesteger

et al. 2001

Environmental Microbiology

View full text Add to dashboard Cite

A two-member co-culture consisting of the dehalorespiring Desulfitobacterium frappieri TCE1 and the sulphate-reducing Desulfovibrio sp. strain SULF1 was obtained via anaerobic enrichment from soil contaminated with tetrachloroethene (PCE). In this co-culture, PCE dechlorination to cis-dichloroethene was due to the activity of the dehalorespiring bacterium only. Chemostat experiments with lactate as the primary electron donor for both strains along with varying sulphate and PCE concentrations showed that the sulphate-reducing strain outnumbered the dehalogenating strain at relatively high ratios of sulphate/PCE. Stable co-cultures with both organisms present at similar cell densities were observed when both electron acceptors were supplied in the reservoir medium in nearly equimolar amounts. In the presence of low sulphate/PCE ratios, the Desulfitobacterium sp. became the numerically dominant strain within the chemostat co-culture. Surprisingly, in the absence of sulphate, strain SULF1 did not disappear completely from the co-culture despite the fact that there was no electron acceptor provided with the medium to be used by this sulphate reducer. Therefore, we propose a syntrophic association between the sulphate-reducing and the dehalorespiring bacteria via interspecies hydrogen transfer. The sulphate reducer was able to sustain growth in the chemostat co-culture by fermenting lactate and using the dehalogenating bacterium as a 'biological electron acceptor'. This is the first report describing growth of a sulphate-reducing bacterium in a defined two-member continuous culture by syntrophically coupling the electron and hydrogen transfer to a dehalorespiring bacterium.

show abstract

“…The natural activities of anaerobic dechlorinating populations can result in reduced toxicity and ultimately in complete clean-up of polluted locations (7,34,35,44). However, in some cases dechlorination may cause problems due to the accumulation of more toxic and/or more mobile dechlorination products that are formed biologically from the primary pollutants (e.g., vinyl chloride formed from tetrachloroethene [PCE] de-halogenation).…”

mentioning

confidence: 99%

Influence of Different Electron Donors and Acceptors on Dehalorespiration of Tetrachloroethene by Desulfitobacterium frappieri TCE1

Gerritse

Drzyzga

Kloetstra

et al. 1999

Appl Environ Microbiol

161

View full text Add to dashboard Cite

Strain TCE1, a strictly anaerobic bacterium that can grow by reductive dechlorination of tetrachloroethene (PCE) and trichloroethene (TCE), was isolated by selective enrichment from a PCE-dechlorinating chemostat mixed culture. Strain TCE1 is a gram-positive, motile, curved rod-shaped organism that is 2 to 4 by 0.6 to 0.8 μm and has approximately six lateral flagella. The pH and temperature optima for growth are 7.2 and 35°C, respectively. On the basis of a comparative 16S rRNA sequence analysis, this bacterium was identified as a new strain of Desulfitobacterium frappieri, because it exhibited 99.7% relatedness to the D. frappieri type strain, strain PCP-1. Growth with H2, formate,l-lactate, butyrate, crotonate, or ethanol as the electron donor depends on the availability of an external electron acceptor. Pyruvate and serine can also be used fermentatively. Electron donors (except formate and H2) are oxidized to acetate and CO2. When l-lactate is the growth substrate, strain TCE1 can use the following electron acceptors: PCE and TCE (to produce cis-1,2-dichloroethene), sulfite and thiosulfate (to produce sulfide), nitrate (to produce nitrite), and fumarate (to produce succinate). Strain TCE1 is not able to reductively dechlorinate 3-chloro-4-hydroxyphenylacetate. The growth yields of the newly isolated bacterium when PCE is the electron acceptor are similar to those obtained for other dehalorespiring anaerobes (e.g.,Desulfitobacterium sp. strain PCE1 andDesulfitobacterium hafniense) and the maximum specific reductive dechlorination rates are 4 to 16 times higher (up to 1.4 μmol of chloride released · min−1 · mg of protein−1). Dechlorination of PCE and TCE is an inducible process. In PCE-limited chemostat cultures of strain TCE1, dechlorination is strongly inhibited by sulfite but not by other alternative electron acceptors, such as fumarate or nitrate.

show abstract

Biodegradation of Trichloroethylene and Dichloromethane in Contaminated Soil and Groundwater

Cited by 18 publications

References 0 publications

Using Tracer Tests to Assess Natural Attenuation of Contaminants along a Channelized Coastal Plain Stream

Using Tracer Tests to Assess Natural Attenuation of Contaminants along a Channelized Coastal Plain Stream

Coexistence of a sulphate‐reducing Desulfovibrio species and the dehalorespiring Desulfitobacterium frappieri TCE1 in defined chemostat cultures grown with various combinations of sulphate and tetrachloroethene

Influence of Different Electron Donors and Acceptors on Dehalorespiration of Tetrachloroethene by Desulfitobacterium frappieri TCE1

Contact Info

Product

Resources

About