Biodegradation of vulcanized rubber by a gut bacterium from plastic-eating mealworms

Cheng, Xiaoyan; Xia, Mengli; Yang, Yu

doi:10.1016/j.jhazmat.2023.130940

Cited by 18 publications

(7 citation statements)

References 42 publications

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“…During the screening process, strain BIT-DXN8 T was isolated from the gut of the coleoptera insect larva Z. atratus . Strain BIT-DXN8 T was found to be able to degrade vulcanized rubber according to the assay procedure used in our recent study [23]. In the present study, strain BIT-DXN8 T is characterized by a polyphasic method for taxonomic purposes.…”

Section: Introductionmentioning

confidence: 86%

“…In our recent study, it was shown that a gut bacterium Acinetobacter sp. BIT-H3 isolated from mealworm gut can grow on and degrade vulcanized rubber [23]. Through genomic and transcriptional analyses, five genes of dszA , dszC1 , dszC2 , Laccase 2147 and Peroxidase1232 were found to be responsible for vulcanized rubber degradation in Acinetobacter sp.…”

Section: Phylogeny and Genome Featuresmentioning

confidence: 99%

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Acinetobacter entericus sp. nov., isolated from the gut of plastic-eating insect larvae Zophobas atratus

Dong,

Yang

2023

International Journal of Systematic and Evolutionary Microbiology

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A Gram-negative, non-motile and rod-shaped strain, BIT-DXN8T, was isolated from the gut of plastic-eating insect larvae Zophobas atratus. The taxonomic position of this new isolate was examined by using a polyphasic approach. A preliminary analysis based on the 16S rRNA gene sequence (1411 bp) indicated that the most similar strain to BIT-DXN8T was Acinetobacter bouvetii DSM 14964T (98.5%), followed by Acinetobacter haemolyticus CIP 64.3T (98.2%) and Acinetobacter pullicarnis S23T (98.2%). The results of phylogenetic analyses, based on the 16S rRNA gene, concatenated sequences of five housekeeping genes (fusA, gyrB, recA, rplB and rpoB) and genome sequences, placed strain BIT-DXN8T in a separate lineage among the genus Acinetobacter of the family Moraxellaceae . The average nucleotide identity and digital DNA–DNA hybridization values of the strain when compared to all other species within the genus Acinetobacter were below 96 and 70 %, respectively. The physiological and biochemical tests confirm the affiliation of strain BIT-DXN8T to the present species within the genus Acinetobacter , but with some specific phenotypic differences. Therefore, strain BIT-DXN8T is considered to represent a novel species, for which the name Acinetobacter entericus sp. nov. is proposed. The type strain is BIT-DXN8T (=CCTCC AB 2022117T=KCTC 92696T).

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Section: Introductionmentioning

confidence: 86%

Section: Phylogeny and Genome Featuresmentioning

confidence: 99%

Acinetobacter entericus sp. nov., isolated from the gut of plastic-eating insect larvae Zophobas atratus

Dong,

Yang

2023

International Journal of Systematic and Evolutionary Microbiology

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“…Approximately 50 mealworms were placed in a polypropylene (PP) box with a capacity of 200 mL. The mealworms were then placed in incubators that were kept at a temperature of 25 °C and a humidity level of 70% (Yang et al 2021b ; Bulak et al 2021 ; Chen et al 2023 ; Cheng et al 2023 ). The mealworms were divided into four experimental groups Fig.…”

Section: Methodsmentioning

confidence: 99%

Biodegradation of polystyrene nanoplastics by Achromobacter xylosoxidans M9 offers a mealworm gut-derived solution for plastic pollution

El-Kurdi,

El-Shatoury,

ElBaghdady

et al. 2024

Arch Microbiol

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Nanoplastics pose significant environmental problems due to their high mobility and increased toxicity. These particles can cause infertility and inflammation in aquatic organisms, disrupt microbial signaling and act as pollutants carrier. Despite extensive studies on their harmful impact on living organisms, the microbial degradation of nanoplastics is still under research. This study investigated the degradation of nanoplastics by isolating bacteria from the gut microbiome of Tenebrio molitor larvae fed various plastic diets. Five bacterial strains capable of degrading polystyrene were identified, with Achromobacter xylosoxidans M9 showing significant nanoplastic degradation abilities. Within 6 days, this strain reduced nanoplastic particle size by 92.3%, as confirmed by SEM and TEM analyses, and altered the chemical composition of the nanoplastics, indicating a potential for enhanced bioremediation strategies. The strain also caused a 7% weight loss in polystyrene film over 30 days, demonstrating its efficiency in degrading nanoplastics faster than polystyrene film. These findings might enhance plastic bioremediation strategies. Graphical abstract

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“…The easiest and most obvious method is the weight loss measurement. To measure the weight loss of a NR sample, the entire residual rubber (including the more intact pieces and broken rubber particles) is usually separated from the rest of the culture and collected and cleaned and dried until the weight is constant ( Cheng et al, 2023 ). The weight loss of NR is equal to the initial weight of the sample minus the residual weight.…”

Section: Investigations and Advancement Of Rubber Biodegradationmentioning

confidence: 99%

“…In the majority of recent investigations, it has been observed that the biodegradation process of NR has shown a rather slow rate, despite the successful identification and isolation of a limited number of functional bacteria capable of degrading this material ( Bosco et al, 2018 ; Joseph et al, 2022; Sarkar et al, 2022 ; Cheng et al, 2023 ). The duration of microbial culture has shown a range of 30–120 days, but assessment methods such as weight loss or SEM have only identified little deterioration.…”

Section: Prospects For Rubber Degradation In the Futurementioning

confidence: 99%

Assembly strategies for rubber-degrading microbial consortia based on omics tools

Cui,

Jiang,

Zhang

et al. 2023

Front. Bioeng. Biotechnol.

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Numerous microorganisms, including bacteria and fungus, have been identified as capable of degrading rubber. Rubber biodegradation is still understudied due to its high stability and the lack of well-defined pathways and efficient enzymes involved in microorganism metabolism. However, rubber products manufacture and usage cause substantial environmental issues, and present physical-chemical methods involve dangerous chemical solvents, massive energy, and trash with health hazards. Eco-friendly solutions are required in this context, and biotechnological rubber treatment offers considerable promise. The structural and functional enzymes involved in poly (cis-1,4-isoprene) rubber and their cleavage mechanisms have been extensively studied. Similarly, novel bacterial strains capable of degrading polymers have been investigated. In contrast, relatively few studies have been conducted to establish natural rubber (NR) degrading bacterial consortia based on metagenomics, considering process optimization, cost effective approaches and larger scale experiments seeking practical and realistic applications. In light of the obstacles encountered during the constructing NR-degrading consortia, this study proposes the utilization of multi-omics tools to discern the underlying mechanisms and metabolites of rubber degradation, as well as associated enzymes and effective synthesized microbial consortia. In addition, the utilization of omics tool-based methods is suggested as a primary research direction for the development of synthesized microbial consortia in the future.

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Biodegradation of vulcanized rubber by a gut bacterium from plastic-eating mealworms

Cited by 18 publications

References 42 publications

Acinetobacter entericus sp. nov., isolated from the gut of plastic-eating insect larvae Zophobas atratus

Acinetobacter entericus sp. nov., isolated from the gut of plastic-eating insect larvae Zophobas atratus

Biodegradation of polystyrene nanoplastics by Achromobacter xylosoxidans M9 offers a mealworm gut-derived solution for plastic pollution

Assembly strategies for rubber-degrading microbial consortia based on omics tools

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