Biodegradation of α-, β-, and γ-Hexachlorocyclohexane by Arthrobacter fluorescens and Arthrobacter giacomelloi

Paolis, M. R. De; Lippi, D.; Guerriero, Ettore; Polcaro, C.; Donati, Enrica

doi:10.1007/s12010-013-0147-9

Cited by 33 publications

(9 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Biotransformation of HCHs was observed in both field sites and laboratory systems. − Over 30 aerobic bacterial strains were reported capable of the complete biodegradation of HCHs. − Anaerobic isolates including Clostridium spp., Desulfovibrio spp., and Desulfococcus spp. can co-metabolically dechlorinate HCHs to tetrachlorocyclohexene (TeCCH), and benzene and chlorobenzene (CB) were detected as final products (for a review, see ref ).…”

Section: Introductionmentioning

confidence: 99%

Dual C–Cl Isotope Analysis for Characterizing the Reductive Dechlorination of α- and γ-Hexachlorocyclohexane by Two Dehalococcoides mccartyi Strains and an Enrichment Culture

Liu

Renpenning

et al. 2020

Environ. Sci. Technol.

View full text Add to dashboard Cite

Hexachlorocyclohexanes (HCHs) are persistent organic contaminants that threaten human health. Microbial reductive dehalogenation is one of the most important attenuation processes in contaminated environments. This study investigated carbon and chlorine isotope fractionation of αand γ-HCH during the reductive dehalogenation by three anaerobic cultures. The presence of tetrachlorocyclohexene (TeCCH) indicated that reductive dichloroelimination was the first step of bond cleavage. Isotope enrichment factors (ε C and ε Cl ) were derived from the transformation of γ-HCH (ε C , from −4.0 ± 0.5 to −4.4 ± 0.6 ‰; ε Cl , from −2.9 ± 0.4 to −3.3 ± 0.4 ‰) and α-HCH (ε C , from −2.4 ± 0.2 to −3.0 ± 0.4 ‰; ε Cl , from −1.4 ± 0.3 to −1.8 ± 0.2 ‰). During α-HCH transformation, no enantioselectivity was observed, and similar ε c values were obtained for both enantiomers. The correlation of 13 C and 37 Cl fractionation (Λ = Δδ 13 C/Δδ 37 Cl ≈ ε C / ε Cl ) of γ-HCH (from 1.1 ± 0.3 to 1.2 ± 0.1) indicates similar bond cleavage during the reductive dichloroelimination by the three cultures, similar to α-HCH (1.7 ± 0.2 to 2.0 ± 0.3). The different isotope fractionation patterns during reductive dichloroelimination and dehydrochlorination indicates that dual-element stable isotope analysis can potentially be used to evaluate HCH transformation pathways at contaminated field sites.

show abstract

Section: Introductionmentioning

confidence: 99%

Dual C–Cl Isotope Analysis for Characterizing the Reductive Dechlorination of α- and γ-Hexachlorocyclohexane by Two Dehalococcoides mccartyi Strains and an Enrichment Culture

Liu

Renpenning

et al. 2020

Environ. Sci. Technol.

View full text Add to dashboard Cite

show abstract

“…DAB-1W100 mg/LThis study2. Streptomyces sp. M716.6 mg/LSineli et al (2016)3. Sphingomonas baderi sp.100 mg/LKaur et al (2013)4. Arthrobacter florescens and Arthrobacter giacomelloi 100 mg/LDe Polis et al (2013)5. Actinobacteria sp. and Streptomyces sp.1.66 mg/L optimum for degradationFuentes et al (2010, 2011)6. Kocuria rhizophila, Microbacterium resistens, Staphylococcus equorum, Staphylococcus cohnii subsp.ureolyticus 5–100 μg/mLAbhilash et al (2011)7.Lindane-degrading consortia300 μg/mL after 108 hElcey and Kunhi (2010)8. Azotobacter chroococcum JL102100 ppmAnupama and Paul (2010)9. Sphingomonas sp.…”

Section: Resultsmentioning

confidence: 99%

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Kumar

Sharma

2016

Bioresour. Bioprocess.

View full text Add to dashboard Cite

BackgroundThis study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.MethodsIn this study the enrichment culture method was used for isolation of lindane degrading bacterial isolates, in which the mineral salt medium (MSM) supplemented with different concentrations of lindane was used. Further, the screening for the potential lindane degrading isolates was done using the spray plate method and colorimetric dechlorinase enzyme assay. The selected isolates were also studied for their growth response under varying range of temperature, pH, and NaCl. The finally selected isolates DAB-1Y and DAB-1W showing best lindane degradation activity was further subjected to biochemical characterization, microscopy, degradation/kinetic study, and 16S rDNA sequencing. The strain identification were performed using the biochemical characterization, microscopy and the species identifies by 16S rDNA sequence of the two isolates using the standard 16S primers, the 16 S rRNA partial sequence was analyzed through BLAST analysis and phylogenetic tree was generated based on UGPMA clustering method using MEGA7 software. This shows the phylogenetic relationship with the related strains. The two isolates of this study were finally characterized as Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W, and their 16S rRNA sequence was submitted to GenBank database with accession numbers, KJ811539 and KX986577, respectively.ResultsOut of the 20 isolates, the isolates DAB-1Y and DAB-1W exhibited best lindane-degrading activity of 94 and 98%, respectively, recorded after 8 days of incubation. The optimum growth was observed at temperature 30 °C, pH 7, and 5% NaCl observed for both isolates. Of the four isomers of hexachlorocyclohexane, isomer α and γ were the fastest degrading isomers, which were degraded up to 86 and 94% by isolates DAB-1Y and up to 93 and 98% by DAB-1W, respectively, reported after 8 days incubation. Isomer β was highly recalcitrant in which maximum 35 and 32% lindane degradation was observed even after 28 days incubation by isolates, DAB-1Y and DAB-1W, respectively. At lower lindane concentrations (1–10 mg/L), specific growth rate increased with increase in lindane concentration, maximum being 0.008 and 0.006/day for DAB-1Y and DAB-1W, respectively. The 16 S rRNA partial sequence of isolate DAB-1Y showed similarity with Kocuria sp. by BLAST analysis and was named as Kocuria sp. DAB-1Y and DAB-IW with Staphylococcus sp. DAB-1W. The 16S rDNA sequence of isolate DAB-1Y and DAB-1W was submitted to online at National Centre of Biotechnology Information (NCBI) with GenBank accession numbers, KJ811539 and KX986577, respectively.ConclusionsThis study has demonstrated that Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W were found efficient in bioremediation of gamma-HCH and can be utilized further for biodegradation of environmental contamination of lindane and can be utilized in bioremediation program.Electronic supplement...

show abstract

“…Among pesticide-degrading actinobacteria, Arthrobacter, Rhodococcus, Streptomyces, Frankia, Janibacter, Kocuria, Mycobacterium, Nocardia, and Pseudonocardia are the most representative. In particular, the genus, Arthrobacter, has been recognised as a degrader of different xenobiotics since members of this group possess various catabolic pathways to detoxify these compounds [107][108][109]. For instance, an Arthrobacter strain (A. chlorophenolicus A6) is a good candidate for 4chlorophenol-degradation activity.…”

Section: Organic Pollutantsmentioning

confidence: 99%

Actinomycetes from Caves: An Overview of Their Diversity, Biotechnological Properties, and Insights for Their Use in Soil Environments

et al. 2022

View full text Add to dashboard Cite

The environmental conditions of caves shape microbiota. Within caves’ microbial communities, actinomycetes are among the most abundant bacteria. Cave actinomycetes have gained increasing attention during the last decades due to novel bioactive compounds with antibacterial, antioxidant and anticancer activities. However, their potential role in soil environments is still unknown. This review summarises the literature dealing with actinomycetes from caves, underlining for the first time their potential roles in soil environments. We provide an overview of their diversity and biotechnological properties, underling their potential role in soil environments applications. The contribution of caves’ actinomycetes in soil fertility and bioremediation and crops biostimulation and biocontrol are discussed. The survey on the literature show that several actinomycetes genera are present in cave ecosystems, mainly Streptomyces, Micromonospora, and Nocardiopsis. Among caves’ actinomycetes, Streptomyces is the most studied genus due to its ubiquity, survival capabilities, and metabolic versatility. Despite actinomycetes’ outstanding capabilities and versatility, we still have inadequate information regarding cave actinomycetes distribution, population dynamics, biogeochemical processes, and metabolisms. Research on cave actinomycetes needs to be encouraged, especially concerning environmental soil applications to improve soil fertility and health and to antagonise phytopathogens.

show abstract

Biodegradation of α-, β-, and γ-Hexachlorocyclohexane by Arthrobacter fluorescens and Arthrobacter giacomelloi

Cited by 33 publications

References 35 publications

Dual C–Cl Isotope Analysis for Characterizing the Reductive Dechlorination of α- and γ-Hexachlorocyclohexane by Two Dehalococcoides mccartyi Strains and an Enrichment Culture

Dual C–Cl Isotope Analysis for Characterizing the Reductive Dechlorination of α- and γ-Hexachlorocyclohexane by Two Dehalococcoides mccartyi Strains and an Enrichment Culture

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

Actinomycetes from Caves: An Overview of Their Diversity, Biotechnological Properties, and Insights for Their Use in Soil Environments

Contact Info

Product

Resources

About