Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil

Zhao, Hai-Ming; Hu, Ruiwen; Chen, Xue-Xue; Chen, Xuebin; Lü, Huixiong; Li, Yanwen; Li, Hui; Chen, Mo; Cai, Quan-Ying; Wong, Ming Hung

doi:10.1016/j.scitotenv.2018.05.334

Cited by 97 publications

(23 citation statements)

References 47 publications

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“…An image of strain YL1 was captured by an atomic force microscope (AFM). Single colonies attached to clean mica slides were observed by AFM [23].…”

Section: Strain Isolation and Identificationmentioning

confidence: 99%

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Wang

et al. 2020

Bioprocess Biosyst Eng

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Sulfamethazine (SM2) is one of the sulfonamide antibiotics that is frequently detected in aquatic environment. Given the complex structure of SM2 and its potential threat to the environment, it is necessary to determine the degradation behavior of high-concentration SM2. The mechanisms of community structure and diversity of activated sludge were analyzed. A novel SM2-degrading strain YL1 was isolated which can degrade SM2 with high concentration of 100 mg L −1. Strain YL1 was identified as Paenarthrobacter ureafaciens and there was also a significant increase in the genus during acclimation. Additional SM2 metabolic mechanisms and genomic information of YL1 were analyzed for further research. The succession of the community structure also investigated the effect of SM2 on the activated sludge. This result not only advances the current understanding of microbial ecology in activated sludge, but also has practical implications for the design and operation of the environmental bioprocesses for treatment of antimicrobial-bearing waste streams.

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“…An image of strain YL1 was captured by an atomic force microscope (AFM). Single colonies attached to clean mica slides were observed by AFM [23].…”

Section: Strain Isolation and Identificationmentioning

confidence: 99%

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Wang

et al. 2020

Bioprocess Biosyst Eng

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“…At present, many DEHP-degrading bacteria have been isolated from different environments, such as Mycobacterium, Nocardia, Pseudomonas, Bacillus, etc. [22,23]. Previous studies on the degradation pathways of DEHP have shown that DEHP may be converted to MEHP through esterase hydrolysis, then further hydrolyzed to PA, and finally gradually mineralized [24][25][26].…”

Section: Introductionmentioning

confidence: 99%

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Zhang

Luo

et al. 2020

Preprint

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A bacterial strain that could effectively degrade DEHP was isolated from the activated sludge and identified as Bacillus sp. by DNA sequencing. The biochemical degradation pathway of DEHP was further analyzed by GC-MS, and the results showed that DEHP was first decomposed into phthalates (DBP). Diuretic sylycol (DEP) was then generated, and phthalates (PA) were generated by a continuous de-ehelateization reaction. Phthalic acid (PA) was oxidized, dehydrogenated, and decarboxylated into protocatechins. Protocatechins enter the TCA cycle through orthotopic ring opening.　To enhance DEHP degradation, sodium alginate and calcium chloride were used as embedding and cross-linking materials, and the strain was immobilized. The immobilization conditions were optimized via an orthogonal experiment, and the results showed that the optimal immobilization conditions were SA mass fraction of 4%, CaCl2 mass fraction of 5%, ratio of bacteria to SA of 1:1, and the crosslinking time of 6 hours. The immobilized bacteria agent was further applied to MBR systems. The results showed that the removal rate of DEHP (5mg/L) in the system by immobilized bacteria was 91.9%, which is significantly higher than that of free bacteria. The 3, 4-dioxygenase gene and microbial community dynamics were analyzed by q-PCR and Illumina Miseq sequencing. The q-PCR results showed that the number of copies of 3, 4-dioxygenase gene in the immobilized system was significantly higher than that of free bacteria. Illumina Miseq sequencing results showed that Micromonospora, Rhodococcus, Bacteroides and Pseudomonas were the dominant generas in the MBR system. The analysis of bacterial community structure indicated that immobilization technology had a positive impact on the system stability. The results implied that this immobilized technique had potential applications in DEHP wastewater treatment.

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“…PAEs are widely detected in the environment, such as in surface water, marine sediments (Yuan et al, 2010;Rabet et al, 2019), municipal wastewater treatment plants (WWTPs) , and sludge (Chang et al, 2007). The compound di-(2-ethylhexyl) phthalate (DEHP) is one of the most important PAEs that is widely used as a plasticizer in building materials and as an additive in cosmetics production (Zhao et al, 2018). DEHP has received increasing attention in recent years due to its interference with the reproductive system (Shih et al, 2018;Aydemir et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the metabolic breakdown of DEHP by microorganisms is currently considered to be one of the major routes of DEHP removal (Wu et al, 2013;Iman et al, 2017). Several degradation bacteria belonging to the genera-Sphingomonas, Pseudomonas, Rhodococcus, Microbacterium, and Gordoniahave been isolated from various environments (Wu et al, 2011;Wang et al, 2012Wang et al, , 2019Jin et al, 2014;Zhao et al, 2018). However, DEHP has a low water solubility and high octanol-water partition coefficient (logKow = 7.5) (Turner and Rawling, 2000), which makes DEHP biodegradation difficult.…”

Section: Introductionmentioning

confidence: 99%

Enhanced Removal of Di-(2-Ethylhexyl) Phthalate Using Cometabolic Substrates in an Expanded Granular Sludge Bed Reactor

Zhang

Yang

Luo

et al. 2020

Environmental Engineering Science

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Recently, the endocrine-disrupting and carcinogenic compound di-(2-ethylhexyl) phthalate (DEHP) has gained increased attention. In this study, the enhanced effect of cometabolism on DEHP removal and the associated microbial processes in an extended granular sludge bed (EGSB) reactor over 293 days were investigated. The results indicated that it took 181 days to start up the reactor, with only a 38.5% removal efficiency when treating 0.6 mg/L DEHP wastewater. The addition of glucose at low concentrations (1 and 2 g/L) promoted DEHP removal. However, higher concentrations (5 and 10 g/L) inhibited the bioprocess. In contrast, yeast enhanced DEHP degradation at all of the tested concentrations (0.1, 0.2, 0.3, and 0.4 g/L). Quantitative real-time PCR (q-PCR) and statistical analysis demonstrated that the amount of the archaea and DEHP removal were positively correlated (r = 0.99, p < 0.05). A MiSeq sequencing analysis revealed that the microbial community had an obvious shift that accompanied the increasing DEHP removal rate. Bacteroidetes and Firmicutes maintained a relatively constant abundance during the entire trial, while Spirochetes and d-Proteobacteria gradually became predominate in the EGSB reactor. At the genus level, the relative abundance of Levilinea, Thauera, and Actinobaculum gradually decreased. For the archaea, the acetoclastic methanogens, Methanosaeta and Methanosarcina, existed in the EGSB reactor throughout the experiment. This study suggests that cometabolism is feasible for enhancing DEHP wastewater treatment, and it shows the potential for practical applications in engineering.

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Biodegradation pathway of di-(2-ethylhexyl) phthalate by a novel Rhodococcus pyridinivorans XB and its bioaugmentation for remediation of DEHP contaminated soil

Cited by 97 publications

References 47 publications

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Biodiversity, isolation and genome analysis of sulfamethazine-degrading bacteria using high-throughput analysis

Biochemical Pathways and Enhanced Degradation of Dioctyl Phthalate (DEHP) by Sodium Alginate Immobilization in MBR System

Enhanced Removal of Di-(2-Ethylhexyl) Phthalate Using Cometabolic Substrates in an Expanded Granular Sludge Bed Reactor

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