The most consistent biodemographic correlate of sexual orientation in men is the number of older brothers (fraternal birth order). The mechanism underlying this effect remains unknown. In this article, I provide a direct test pitting prenatal against postnatal (e.g., social͞rearing) mechanisms. Four samples of homosexual and heterosexual men (total n ؍ 944), including one sample of men raised in nonbiological and blended families (e.g., raised with halfor step-siblings or as adoptees) were studied. Only biological older brothers, and not any other sibling characteristic, including nonbiological older brothers, predicted men's sexual orientation, regardless of the amount of time reared with these siblings. These results strongly suggest a prenatal origin to the fraternal birthorder effect.immune ͉ sexuality R ecent research has provided evidence that genetic and prenatal factors may influence sexual orientation development (1-7). In this article, I demonstrate that the number of biological older brothers, including those not reared with the participant (but not the number of nonbiological older brothers), increases the probability of homosexuality in men. These results provide evidence that a prenatal mechanism(s), and not social and͞or rearing factors, affects men's sexual orientation development.The most consistent biodemographic correlate of sexual orientation in men is the number of older brothers, originally observed by Blanchard and Bogaert (8) in a Canadian sample in the 1990s but since then found in samples from different eras and from different countries, both by us and independent investigators (2, 7, 9-12). Evidence does not exist that sibling characteristics reliably correlate with women's sexual orientation (13,14). Both childhood social͞rearing (13, 15, 16) and prenatal (8, 13) mechanisms have been advanced to account for the older brother (''fraternal birth-order'') effect in men, but a direct test pitting prenatal versus postnatal (e.g., social͞rearing) mechanisms is lacking. Such a test is possible when information on both biological and nonbiological siblings, along with sexual orientation, is included in the research design.Four samples of homosexual and heterosexual men (total n ϭ 944) reporting on their parental and sibling characteristics (i.e., biological and nonbiological siblings) were examined to test this issue. Three samples were archival and contained men with (largely) biologically intact families. These samples contained information on all siblings (both biological and nonbiological) with whom the participant was reared. The fourth sample was recruited specifically to test the research issue investigated in this article and contained men with nonbiological or blended families (e.g., raised with half-or step-siblings or as adoptees). This final sample also contained information on the amount of time the participants were reared with each sibling, along with information on any biological siblings with whom they were never reared.If rearing or social factors associated with older m...