Biodesulfurization of a System Containing Synthetic Fuel Using Rhodococcus erythropolis ATCC 4277

Maass, Danielle; Oliveira, Débora de; Souza, Sabrina da Silva de; Souza, Selene M.A. Guelli U. de

doi:10.1007/s12010-014-1189-3

Cited by 17 publications

(16 citation statements)

References 22 publications

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“…Strains of Rhodococcus erythropolis have been identified as the most promising bacteria for biodesulfurization. For example, strain ATCC 4277 is most effective for the desulfurization of dibenzothiophene (DBT) (7). Moreover, R. erythropolis CCM2595 is studied as a model strain for the bioremediation of phenol and other aromatic compounds (8).…”

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confidence: 99%

Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp

Khairy

Wübbeler

Steinbüchel

2015

Appl Environ Microbiol

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c Four Rhodococcus spp. exhibited the ability to use 4,4=-dithiodibutyric acid (DTDB) as a sole carbon source for growth. The most important step for the production of a novel polythioester (PTE) using DTDB as a precursor substrate is the initial cleavage of DTDB. Thus, identification of the enzyme responsible for this step was mandatory. Because Rhodococcus erythropolis strain MI2 serves as a model organism for elucidation of the biodegradation of DTDB, it was used to identify the genes encoding the enzymes involved in DTDB utilization. To identify these genes, transposon mutagenesis of R. erythropolis MI2 was carried out using transposon pTNR-TA. Among 3,261 mutants screened, 8 showed no growth with DTDB as the sole carbon source. In five mutants, the insertion locus was mapped either within a gene coding for a polysaccharide deacetyltransferase, a putative ATPase, or an acetyl coenzyme A transferase, 1 bp upstream of a gene coding for a putative methylase, or 176 bp downstream of a gene coding for a putative kinase. In another mutant, the insertion was localized between genes encoding a putative transcriptional regulator of the TetR family (noxR) and an NADH:flavin oxidoreductase (nox). Moreover, in two other mutants, the insertion loci were mapped within a gene encoding a hypothetical protein in the vicinity of noxR and nox. The interruption mutant generated, R. erythropolis MI2 nox⍀tsr, was unable to grow with DTDB as the sole carbon source. Subsequently, nox was overexpressed and purified, and its activity with DTDB was measured. The specific enzyme activity of Nox amounted to 1.2 ؎ 0.15 U/mg. Therefore, we propose that Nox is responsible for the initial cleavage of DTDB into 2 molecules of 4-mercaptobutyric acid (4MB).T he genus Rhodococcus has attracted great interest in recent years due to the ability of many of its species to degrade and transform a wide range of xenobiotic substances (1). Rhodococci are described as aerobic, Gram-positive, mycolate-containing actinomycetes with high genomic GϩC contents. They have a diverse set of genetic and catabolic features, because of the presence of large linear plasmids in addition to their large chromosomes (2). The number of publications and patents involving Rhodococcus spp. has increased significantly in recent years (3).Rhodococcus spp. have been isolated from a variety of sources. They are ubiquitous in soils contaminated with crude oil and/or other xenobiotic compounds. The ability of Rhodococcus species to degrade substituted hydrocarbons and other chemicals allows them to play a role in the natural degradation and bioremediation of such compounds (4). Many Rhodococcus species are characterized by broad metabolic diversity and an array of unique enzymatic capabilities; therefore, they are also of interest for pharmaceutical, environmental, chemical, and energy studies. They play a significant role in the process of desulfurization of fossil fuels (5) and in the industrial production of acrylamide (6). Strains of Rhodococcus erythropolis have been identi...

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confidence: 99%

Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp

Khairy

Wübbeler

Steinbüchel

2015

Appl Environ Microbiol

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“…1 and 2, Monod model portrayed appropriately the evolution of biomass concentration, DBT degradation, glucose consumption, and 2-HBP production, principally during to logarithmic phase. The modeling of the phenomena associated with logarithmic phase appears to be the most important in these BDS experiments since the highest rates of DBT degradation and 2-HBP production occur in this growth phase [31].…”

Section: Resultsmentioning

confidence: 99%

“…The kinetic models applied to BDS process were evaluated based on experimental data previously published [31], where the desulfurization capacity of R. erythropolis ATCC 4277 was tested in a batch reactor using 20, 80, and 100 % (v/v) of organic phase (dodecane) and 3.0 mM of DBT. The aqueous phase was composed by a Yeast Malt Agar medium (YMA) comprised with 3.0 g L −1 of yeast extract, 3.0 g L −1 of malt extract, 5.0 g L −1 of bactopeptone, 10.0 g L −1 of glucose, and 2.0 g L −1 of calcium carbonate.…”

Section: Mathematical Modelsmentioning

confidence: 99%

An Evaluation of Kinetic Models in the Biodesulfurization of Synthetic Oil by Rhodococcus erythropolis ATCC 4277

Maass

Mayer

Moritz

et al. 2015

Appl Biochem Biotechnol

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Biodesulfurization is an eco-friendly technology applied in the removal of sulfur from fossil fuels. This technology is based on the use of microorganisms as biocatalysts to convert the recalcitrant sulfur compounds into others easily treatable, as sulfides. Despite it has been studied during the last decades, there are some unsolved questions, as per example the kinetic model which appropriately describes the biodesulfurization globally. In this work, different kinetic models were tested to a batch desulfurization process using dibenzothiophene (DBT) as a model compound, n-dodecane as organic solvent, and Rhodococcus erythropolis ATCC 4277 as biocatalyst. The models were solved by ODE45 function in the MATLAB. Monod model was capable to describe the biodesulfurization process predicting all experimental data with a very good fitting. The coefficients of determination achieved to organic phase concentrations of 20, 80, and 100 % (v/v) were 0.988, 0.995, and 0.990, respectively. R. erythropolis ATCC 4277 presented a good affinity with the substrate (DBT) since the coefficients of saturation obtained to reaction medium containing 20, 80, and 100 % (v/v) were 0.034, 0.07, and 0.116, respectively. This kinetic evaluation provides an improvement in the development of biodesulfurization technology because it showed that a simple model is capable to describe the throughout process.

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“…When compared with other strains, Rhodococcus sp. presents a greater specific degradation rate and stability during BDS process [11,16,[21][22][23][24][25][26][27].…”

Section: Introductionmentioning

confidence: 99%

“…For practical purposes, development of BDS and BDN requires the use of real products and byproducts from petroleum distillation such as diesel, gasoline, and gas oils [11,[22][23][24][27][28][29][30]. The heavy gas oil (HGO) is an intermediate fraction obtained from the vacuum distillation used in the production of diesel and some lubricants.…”

Section: Introductionmentioning

confidence: 99%

Desulfurization and denitrogenation of heavy gas oil by Rhodococcus erythropolis ATCC 4277

Maass

Todescato

Moritz

et al. 2015

Bioprocess Biosyst Eng

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Some of the noxious atmospheric pollutants such as nitrogen and sulfur dioxides come from the fossil fuel combustion. Biodesulfurization and biodenitrogenation are processes which remove those pollutants through the action of microorganisms. The ability of sulfur and nitrogen removal by the strain Rhodococcus erythropolis ATCC 4277 was tested in a biphasic system containing different heavy gas oil concentrations in a batch reactor. Heavy gas oil is an important fraction of petroleum, because after passing through, the vacuum distillation is incorporated into diesel oil. This strain was able to remove about 40% of the nitrogen and sulfur present in the gas heavy oil. Additionally, no growth inhibition occurred even when in the presence of pure heavy gas oil. Results present in this work are considered relevant for the development of biocatalytic processes for nitrogen and sulfur removal toward building feasible industrial applications.

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Biodesulfurization of a System Containing Synthetic Fuel Using Rhodococcus erythropolis ATCC 4277

Cited by 17 publications

References 22 publications

Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp

Biodegradation of the Organic Disulfide 4,4′-Dithiodibutyric Acid by Rhodococcus spp

An Evaluation of Kinetic Models in the Biodesulfurization of Synthetic Oil by Rhodococcus erythropolis ATCC 4277

Desulfurization and denitrogenation of heavy gas oil by Rhodococcus erythropolis ATCC 4277

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