Biodesulfurization of dibenzothiophene by Shewanella putrefaciens NCIMB 8768

Abstract

The desulfurization ability of Shewanella putrefaciens strain NCIMB 8768 was studied and its activity profile was compared with the widely studied strain Rhodococcus erythropolis strain IGTS8. Dibenzothiophene (DBT) is a recalcitrant thiophenic component of fossil fuels especially among diesel blend stocks. DBT in basic salt medium (BSM) at a final concentration of 0.3, 0.6 and 0.9 mM was supplied to the microbes as the sole sulfur source. Experimental results showed that S. putrefaciens, similar to other biodesulfurization organisms, converted DBT to the end product 2-hydroxybiphenyl (HBP), as detected by the Gibbs assay and HPLC. Cells cultivated in medium containing 0.3 mM of DBT showed the highest desulfurization activity, with a maximum specific production rate 43.5 mmol/L of HBP.

A novel Bacillus pumilus-related strain from tropical landfarm soil is capable of rapid dibenzothiophene degradation and biodesulfurization

“…Alternatively, DBT and/or its derivative metabolites might have been used as carbon source in an alternative pathway [ 16 - 18 ], thereby preventing full operation of the 4S pathway, and so, the production/accumulation of HBP. At least as observed for the culture starting with 0.5-OD inoculum, the rapid DBT consumption and detection of HBP could corroborate previous findings about the action of 4S-pathway enzymes during exponential growth phase [ 6 , 23 - 26 , 33 , 34 ]. Although the HBP detection data would also indicate that genes related to this metabolism (such as dsz C and dsz B) should be operational, homologous sequences for these genes were lacking (see above), which suggest the existence of a specific mechanism and genes not yet described for DBT consumption/HBP formation in the B. pumilus -like RR-3 isolate.…”

“… Gai, et al [ 23 ] 82 48 Stenotrophomonas sp. NISOC-04 Papizadeh et al [ 6 ] 50 120 Microbacterium strain ZD-M2 Li et al [ 24 ] 58 80 Alcaligenes denitrificans ( subsp ) Van Afferden et al [ 16 ] 100 75 Rhodococcus erythropolis SHT87 Davoodi-Dehaghani et al [ 25 ] 60 120 Shewanella putrefaciens NCIMB 8768 Ansari et al [ 26 ] 83 120 Rhodococcus erythropolis IGTS8 Ansari et al [ 26 ] 75 36 Rhodosporidium toruloides DBVPG 6662 2 Baldi, et al [ 27 ] 99.9 24 Bacillus sp. RR-3 This study 1 Time of culturing/incubation in which the corresponding % of DBT degradation on the left column was obtained.…”

DBT degradation enhancement by decorating Rhodococcus erythropolis IGST8 with magnetic Fe3O4 nanoparticles

“…R. erythropolis is known to be able to utilize a wide variety of sulfur compounds as sulfur sources (Kayser et al, 1993) and this microorganism has been investigated in some detail (Ansari et al, 2007;Gray et al, 1996;Li et al, 1996). We suspended R. erythropolis in basic salt medium (BSM) containing DBT as the sole source of sulfur, and the DBT degradation ability of R. erythropolis was measured with the help of highperformance liquid chromatography (HPLC).…”

Biodegradation of dibenzothiophene and its application in the production of clean coal

“…In addition to DBT, the bacterial isolate SHT87 was also found capable of utilizing DBT sulphone, thiophene, 2-methylthiophene and dimethylsulphoxide as a sole source of sulphur for growth [51]. Similarly, a bacterial strain, Shewanella putrefaciens NCIMB8768 has been reported to achieve a maximum production rate of 43.5 mmol/L 2-HBP in 72 h, when provided with 0.3 mM DBT [52]. A novel strain, Microbacterium sp.…”