Biodiesel derived waste glycerol as an economic substrate for biosurfactant production using indigenous Pseudomonas aeruginosa

Bharali, Pranjal; Singh, Salam Pradeep; Dutta, Nippu; Gogoi, Sanjib; Bora, Limpon; Debnath, Palash; Konwar, Bolin Kumar

doi:10.1039/c4ra05594b

Cited by 29 publications

(20 citation statements)

References 30 publications

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“…[1][2][3][4][5] These properties and ecological awareness of end-users have led to an upsurge in application of biosurfactants in dairy, food, beverage, cosmetics, detergent, textile, paint, mining, petroleum, paper/pulp, bioremediation, pharmaceuticals, biomedicine and nanotechnology. [1][2][3][4][5] These properties and ecological awareness of end-users have led to an upsurge in application of biosurfactants in dairy, food, beverage, cosmetics, detergent, textile, paint, mining, petroleum, paper/pulp, bioremediation, pharmaceuticals, biomedicine and nanotechnology.…”

Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] These properties and ecological awareness of end-users have led to an upsurge in application of biosurfactants in dairy, food, beverage, cosmetics, detergent, textile, paint, mining, petroleum, paper/pulp, bioremediation, pharmaceuticals, biomedicine and nanotechnology. 2,3 The vegetable oil refineries (~90) in India manufacture oil from soybean, sunflower, olive, groundnut, rapeseed, safflower, cotton, sesame, coconut, palm, mustard, rice bran, watermelon, etc. 2,3 The vegetable oil refineries (~90) in India manufacture oil from soybean, sunflower, olive, groundnut, rapeseed, safflower, cotton, sesame, coconut, palm, mustard, rice bran, watermelon, etc.…”

Section: Introductionmentioning

confidence: 99%

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Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity

2015

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A total of 76 bacterial isolates were screened for biosurfactant activity using 16 different high throughput screening methods. Based on phenotypic characteristics, 16S rDNA sequencing and FAME profiling, isolate BS02, identified as Bacillus clausii BS02 produced a biosurfactant (2.6 g l -1 ) when grown in basal salts mineral medium with sunflower oil soapstock (3% w/v) at 30 o C and 120 rpm after 42 h. The cell free broth showed 82% emulsification activity, critical micelle concentration of 45 mg l -1 and reduced the surface tension from 69.07 mN m -1 to 30 mN m -1 . Acid precipitated and methanol extracted crude biosurfactant had 64.3%, 28%, and 4.5% (w/w) of protein, lipid, and carbohydrate, respectively. Crude biosurfactant exhibited stability with oils, fats and non-aqueous phase liquids. Fourier transform infrared (FT-IR) spectroscopy, energy dispersive X-ray spectroscopy (EDX), and nuclear magnetic resonance spectroscopy (NMR) revealed the functional groups and bonds; X-ray diffraction (XRD) and thermogravimetry (TG) showed surface nature and thermostability of the biosurfactant, respectively. LC-ESI-MS identified the crude biosurfactant as a surfactin lipopeptide. A dose-dependent mortality against C. chinensis and M. hirsutus was observed using the crude biosurfactant, with a LC 50 of 50 µg ml -1 , indicating first evidence of insecticidal activity against pulse beetle and mealybug.

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Section: Introductionmentioning

confidence: 99%

“…[1][2][3][4][5] These properties and ecological awareness of end-users have led to an upsurge in application of biosurfactants in dairy, food, beverage, cosmetics, detergent, textile, paint, mining, petroleum, paper/pulp, bioremediation, pharmaceuticals, biomedicine and nanotechnology. 2,3 The vegetable oil refineries (~90) in India manufacture oil from soybean, sunflower, olive, groundnut, rapeseed, safflower, cotton, sesame, coconut, palm, mustard, rice bran, watermelon, etc. 2,3 The vegetable oil refineries (~90) in India manufacture oil from soybean, sunflower, olive, groundnut, rapeseed, safflower, cotton, sesame, coconut, palm, mustard, rice bran, watermelon, etc.…”

Section: Introductionmentioning

confidence: 99%

Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity

2015

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“…The preliminary characterization of this type of biosurfactant was a glycolipid (15). Three distinct peaks (0.17, 0.34 and 0.53) were observed in the study conducted by Bharali et al, (4) to produce RL from P. aeruginosa grown on biodiesel, while only two spots (R f of 0.9 and 0.6) observed by Das et al, (11) for mono and di-RL, respectively. These differences in R f values is correlated to the congeners of the biosurfactant mixture depending on the production isolate and growth conditions.…”

Section: Extraction Of Rlmentioning

confidence: 90%

Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

Faqri¹

2019

Iraqi J. Agric. Sci.

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This study was depend to select Pseudomonas aeruginosa A3 as a good producer of rhamnolipid (RL) biosurfactant after screening on agar plate where it was able to biosynthesize 4.3 g/L with emulsification index 52% and reducing the surface tension of water to 33.2 mN/m. Therefore, this study was aimed to increase the production of biosurfactant from selected isolate by exposure to several physical and chemical mutagenic factors like gamma radiation, nitrosoguanidine, ethyl methane sulfonate and lithium chloride. The results were shown that 0.2 g/L of nitrosoguanidine was the best mutant for increasing the production to about 2 folds (9.4 g/L) after 15 mins exposure to this material, as well as the emulsification index and surface tension of water were reached to 62% and 26.1 mN/m, respectively, comparing with non-mutant isolate. Also, the critical micelle concentration (CMC) and critical micelle dilution (CMD) of produced rhamnolipid were reached to 120 mg/L and 100 fold, respectively. The optimum conditions of RL production from mutant isolate were determined as 34ºC a best temperature, 6.5 optimum pH and incubation period of 108 h where the production was reached to 10.6 g/L and emulsification index 64% with surface tension of water 26 mN/m. characterization study of purified RL by using thin layer chromatography (TLC) analysis indicated that it was composed of a mixture of mono and di-rhamnolipid.

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“…5 It is recognised that using microbial enhanced oil recovery (MEOR) provides new challenges to extract more oil out of existing reservoirs. 6,8 Such compounds are commonly called amphiphilic or amphipathic molecules. 6,7 Note that metabolites are the intermediates and products of bacterial metabolism such as biosurfactants, biogas, bioacids, biosolvents and biopolymers.…”

Section: Introductionmentioning

confidence: 99%

Laboratory simulation of microbial enhanced oil recovery using Geobacillus toebii R-32639 isolated from the Handil reservoir

2015

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Microbial enhanced oil recovery (MEOR) is a biological based technology to enhance crude oil (CO) recovery at old oil wells by manipulating function or structure or both of the microbial environments existing in oil reservoirs. Even if many biosurfactant-producing microbes can be found in different environments, the ability of Geobacillus toebii R-32639 to enhance CO recovery needs to be verified. In this study, artificial cores (ACs) are used to simulate the application of MEOR by three scenarios of injecting nutrients, microbial culture and bioproducts. The use of Geobacillus toebii R-32639 as biosurfactant producer enables a lowering by 25.3% of interfacial tension and by 14.1% of CO viscosity and also enables the degrading of a 7.4-28.8% fraction of (C 12 -C 34 ) hydrocarbons after 7 days of incubation. The injection of microbial culture into a laboratory-scale CO reservoir of the AC for the desirable purpose of enhanced oil recovery could feasibly achieve an average recovery factor of 14.27% but lower by 5.50% the AC porosity and by 91.0% the AC permeability; this is the best-case scenario of the microbial core flooding (MCF) experiments. The simulation of MEOR by using bacterial strains of Geobacillus toebii R-32639 provides valuable insight into the use of MCF experiments to obtain greater oil production from existing reservoirs.

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Biodiesel derived waste glycerol as an economic substrate for biosurfactant production using indigenous Pseudomonas aeruginosa

Abstract: Biodiesel plant waste glycerol as low-cost substrate for biosurfactant production.

Cited by 29 publications

References 30 publications

Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity

Lipopeptide biosurfactant from Bacillus clausii BS02 using sunflower oil soapstock: evaluation of high throughput screening methods, production, purification, characterization and its insecticidal activity

Induction of Rhamnolipid Production by Pseudomonas Aeruginosa A3 Using Chemical and Physical Mutagenic Factors

Laboratory simulation of microbial enhanced oil recovery using Geobacillus toebii R-32639 isolated from the Handil reservoir

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