Biodiesel from oleaginous microbes: opportunities and challenges

Gujjala, Lohit Kumar Srinivas; Kumar, Sanjay; Talukdar, Bitasta; Dash, Abhisek; Kumar, Sanjeev; Sherpa, Knawang Chhunji

doi:10.1080/17597269.2017.1402587

Cited by 43 publications

(22 citation statements)

References 123 publications

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“…Lipid-based biofuels have attracted more attention than petroleum-based fuels, after growing energy demand and global warming concerns [ 8 ]. Oleaginous microorganisms are promising sources for production of lipid-based biofuels [ 9 ]. For instance, oleaginous yeast strains can accumulate intracellular lipid up to 80% of their dry cell weight, which can be utilized for production of lipid-based biofuels [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Rhodosporidium sp. DR37: a novel strain for production of squalene in optimized cultivation conditions

Shakeri

Khoshbasirat

Maleki

2021

Biotechnol Biofuels

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Background Rhodosporidium strain, a well-known oleaginous yeast, has been widely used as a platform for lipid and carotenoid production. However, the production of squalene for application in lipid-based biofuels is not reported in this strain. Here, a new strain of Rhodosporidium sp. was isolated and identified, and its potential was investigated for production of squalene under various cultivation conditions. Results In the present study, Rhodosporidium sp. DR37 was isolated from mangrove ecosystem and its potential for squalene production was assessed. When Rhodosporidium sp. DR37 was cultivated on modified YEPD medium (20 g/L glucose, 5 g/L peptone, 5 g/L YE, seawater (50% v/v), pH 7, 30 °C), 64 mg/L of squalene was produced. Also, squalene content was obtained as 13.9% of total lipid. Significantly, use of optimized medium (20 g/L sucrose, 5 g/L peptone, seawater (20% v/v), pH 7, 25 °C) allowed highest squalene accumulation (619 mg/L) and content (21.6% of total lipid) in Rhodosporidium sp. DR37. Moreover, kinetic parameters including maximum specific cell growth rate (μmax, h−1), specific lipid accumulation rate (qp, h−1), specific squalene accumulation rate (qsq, h−1) and specific sucrose consumption rate (qs, h−1) were determined in optimized medium as 0.092, 0.226, 0.036 and 0.010, respectively. Conclusions This study is the first report to employ marine oleaginous Rhodosporidium sp. DR37 for accumulation of squalene in optimized medium. These findings provide the potential of Rhodosporidium sp. DR37 for production of squalene as well as lipid and carotenoids for biofuel applications in large scale. Graphic abstract

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Section: Introductionmentioning

confidence: 99%

Rhodosporidium sp. DR37: a novel strain for production of squalene in optimized cultivation conditions

Shakeri

Khoshbasirat

Maleki

2021

Biotechnol Biofuels

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“…In fungi there are two mechanisms for lipid synthesis: Ex novo and de novo (Carsanba et al, 2018). In oleaginous fungi excess of carbon in the form hydrophilic substrates such as glucose in the culture media plays an important role in de-novo lipid synthesis (Wynn et al, 2001;Arous et al, 2015;Gujjala et al, 2019;Huang et al, 2018). While excess of carbon in the form of hydrophobic substrates such as cooking oils plays important role in ex-novo lipid biosynthesis (Subramaniam et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

The Effect of Soybean Oil on Lipid Metabolism in Mucor circinelloides WJ11 by Metabolomic Analysis

Sun¹,

Shah²,

Yang³

et al. 2021

American Journal of Biochemistry and Biotechnology

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Oleaginous fungus Mucor circinelloides utilizes different carbon sources associated with sustainable production of lipids, using soybean oil as carbon source, the fungus accumulated more intracellular lipids. Nonetheless, the metabolic changes in M. circinelloides upon vegetable oil as main carbon source have not been yet reported. Therefore, this study was conducted to investigate the metabolomics of M. circinelloides WJ11 cultivated in mixed glucose and soybean oil as carbon source and to reveal its effects on lipid metabolism. M. circinelloides strain WJ11 was cultured under optimized conditions in a fermenter and the biomass samples were collected after 24 h for lipid metabolite investigation. The frozen biomass samples were subjected to various chromatographic analyses like liquid chromatography mass spectrometry analysis and high resolution-mass spectrometry for metabolite profiling. A total of 438 differential metabolites were identified, of which 48 were up-regulated and 33 were down-regulated. Among them, lysophosphatidic acid and monoglyceride were up-regulated whereas phosphatidylglycerol and lyso-phosphatidylglycerol were down-regulated in the experimental group (soybean oil and glucose as mixed carbon sources) compared with the control group (glucose as single carbon source). Significant changes in metabolite levels correlated to lipid synthesis were identified. This study showed that the addition of soybean oil to the medium favors the triacylglycerol synthesis in M. circinelloides. Our results can also be applied to the investigation of other microorganisms and would contribute to further genetic engineering for higher lipid accumulation in fungi.

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“…Microbial lipids' components are similar to that of vegetable oils, while have several advantages over vegetable oils [17,18]. Such as a short life cycle, low space demands and independent of location and climates [19,20]. Thus, the S. pararoseus also has been considered as potential feed stock for biodiesel industry [8].…”

Section: Introductionmentioning

confidence: 99%

Whole genome sequencing and comparative genomic analysis of oleaginous red yeast Sporobolomyces pararoseus NGR identifies candidate genes for biotechnological potential and ballistospores-shooting

Zhao

et al. 2020

BMC Genomics

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Background: Sporobolomyces pararoseus is regarded as an oleaginous red yeast, which synthesizes numerous valuable compounds with wide industrial usages. This species hold biotechnological interests in biodiesel, food and cosmetics industries. Moreover, the ballistospores-shooting promotes the colonizing of S. pararoseus in most terrestrial and marine ecosystems. However, very little is known about the basic genomic features of S. pararoseus. To assess the biotechnological potential and ballistospores-shooting mechanism of S. pararoseus on genome-scale, the whole genome sequencing was performed by next-generation sequencing technology. Results: Here, we used Illumina Hiseq platform to firstly assemble S. pararoseus genome into 20.9 Mb containing 54 scaffolds and 5963 predicted genes with a N50 length of 2,038,020 bp and GC content of 47.59%. Genome completeness (BUSCO alignment: 95.4%) and RNA-seq analysis (expressed genes: 98.68%) indicated the high-quality features of the current genome. Through the annotation information of the genome, we screened many key genes involved in carotenoids, lipids, carbohydrate metabolism and signal transduction pathways. A phylogenetic assessment suggested that the evolutionary trajectory of the order Sporidiobolales species was evolved from genus Sporobolomyces to Rhodotorula through the mediator Rhodosporidiobolus. Compared to the lacking ballistospores Rhodotorula toruloides and Saccharomyces cerevisiae, we found genes enriched for spore germination and sugar metabolism. These genes might be responsible for the ballistospores-shooting in S. pararoseus NGR. Conclusion: These results greatly advance our understanding of S. pararoseus NGR in biotechnological potential and ballistospores-shooting, which help further research of genetic manipulation, metabolic engineering as well as its evolutionary direction.

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Biodiesel from oleaginous microbes: opportunities and challenges

Cited by 43 publications

References 123 publications

Rhodosporidium sp. DR37: a novel strain for production of squalene in optimized cultivation conditions

Rhodosporidium sp. DR37: a novel strain for production of squalene in optimized cultivation conditions

The Effect of Soybean Oil on Lipid Metabolism in Mucor circinelloides WJ11 by Metabolomic Analysis

Whole genome sequencing and comparative genomic analysis of oleaginous red yeast Sporobolomyces pararoseus NGR identifies candidate genes for biotechnological potential and ballistospores-shooting

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