Biodiesel production from Stichococcus strains at laboratory scale

Abstract

BACKGROUND: This paper reports the results of an experimental campaign of autotrophic cultures of Stichococcus strains aiming at selecting the most promising strain for biofuel production. The strain selected -S. bacillaris 158/11 -was cultivated in 1 L lab-scale bubble column photobioreactors under fed-batch and semi-continuous conditions. A Bold basal medium supplemented with NaNO 3 as nitrogen source was adopted. Tests were carried out at 23• C, 140 µE m −2 s −1 , and air flow rate ranging between 0.4 and 4 vvm. Cultures were characterized in terms of pH, concentration of total nitrogen, total organic carbon, total inorganic carbon, biomass, lipid fraction and methyl-ester distribution of transesterified lipids.

Temperature responses of growth, photosynthesis, fatty acid and nitrate reductase in Antarctic and temperate Stichococcus

“…1 throughout Antarctica (Vinocur and Izaguirre 1994;Broady 1996;Massalski et al 2001) and its physiological and behavioral adaptations allow it to exist in the most extreme environments (Hughes 2006). In addition, Stichococcus is a potential candidate for biodiesel production for its high biomass and lipid content under autotrophic conditions (Olivieri et al 2011). In this study, big lipid droplets were observed in the cytoplasm of S. bacillaris NJ-10 and S. minutus NJ-17 together with a good adaptation to the extreme environments, which indicated that the two Antarctic strains might serve as feedstocks for biodiesel production.…”

“…Stichococcus bacillaris, the model species of this genus, distributes all over the world including Antarctica, showing an extensive adaptability to changing temperatures, salinities and pH values with short life cycles (Pollio et al 1997). Moreover, Stichococcus species have been identified to be a promising alternative feedstock for biodiesel production owing to the high lipid content together with high growth rate and biomass (Olivieri et al 2011). Many species of genus Stichococcus have been isolated from Antarctica, which shows that these show good adaptation to the extremely low temperature (Vinocur and Izaguirre 1994;Broady 1996;McKnight et al 2000;Massalski et al 2001;Teoh et al 2004;Hughes 2006).…”

Photobioreactors for microalgal cultures: A Lagrangian model coupling hydrodynamics and kinetics

“…Indeed, the metabolism of these microorganisms is based on the photosynthetic upper‐pathway and is the most effective route to produce biomass—to be adopted as energy source, food, and feed—from sunlight. Microalgae can be cultivated on waste streams of gas (CO 2 polluted) and liquids (salt supplements) for producing bio‐oil, biodiesel, biohydrogen, biobutanol, food additives, pigments, and cosmetics and at the same time to contribute to the CO 2 capture . Their intensive cultures are typically carried out in photobioreactors: closed reactors operated under controlled conditions.…”

Çeşitli yetiştirme ortamlarında ek besin olarak kullanılan peynir altı suyunun biyodizel hammaddesi Chlorella vulgaris’in üretim potansiyeline etkisinin belirlenmesi

“…After centrifugation, sample material was kept at 60 0 C for 12h and the amount of fat was determined using a Soxlet device (Olivieri et al, 2011).…”