Biodiesel production from lipid of carbon dioxide sequestrating bacterium and lipase of psychrotolerant Pseudomonas sp. ISTPL3 immobilized on biochar

Khosla, Khushboo; Rathour, Rashmi; Maurya, Raj; Maheshwari, Neha; Gnansounou, Edgard; Larroche, Christian; Thakur, Indu Shekhar

doi:10.1016/j.biortech.2017.08.194

Cited by 64 publications

(19 citation statements)

References 46 publications

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“…Pseudomonas , Janthinobacterium , Arthrobacter , and Flavobacterium occupied the highest abundance in R 20 , R 30 , and R 50 , which agreed with the activated sludge system at low temperatures 23 , 43 . Among them, Pseudomonas , Arthrobacter , and Flavobacterium were enriched in biochar with a high ammonification ability in the soil 44 , 45 . This bacterial genus enrichment might be a result of specific biochar surface characteristics, which concurrently partition microorganism spatial distribution, and prompt microorganism absolute abundance, as well as microbial community’s structural stability 46 .…”

Section: Discussionmentioning

confidence: 99%

Nitrogen loading effects on nitrification and denitrification with functional gene quantity/transcription analysis in biochar packed reactors at 5 °C

Ding

Pan

et al. 2018

Sci Rep

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This study investigated the nitrogen transformation rates of different nitrogen-loading (20, 30, and 50 mg TN/L) biochar packed reactors (C:N:P = 100:5:1) within 125 days at 5 °C. The results showed that high nitrogen loading resulted in an NH4+ (TN) removal efficiency decline from 98% (57%) to 83% (29%), with biochar yielding a higher NH4+, TN and DON removal rate than conventional activated sludge. Moreover, all biochar packed reactors realized a quick start-up by dropping in temperature stage by stage, and the effluent dissolved organic nitrogen (DON) concentrations of R20, R30, and R50 were 0.44 ± 0.18, 0.85 ± 0.35, and 0.66 ± 0.26 mg/L, respectively. The nirS/amoA, nxrA/amoA, and amoA/(narG + napA) were deemed to be the markers of ammonium oxidation rate (SAOR), specific nitrite oxidation rate (SNOR), and specific nitrate reduction rate (SNRR), respectively. Compared with functional gene quantity data, transcription data (mRNA) introduced into stepwise regression analyses agreed well with nitrogen transformation rates. High nitrogen loading also resulted in the cell viability decreased in R50. Nitrogen loadings and operation time both led to a significant variation in cell membrane composition, and unsaturated fatty acids (UFAs) significantly increased in R30 (46.49%) and R50 (36.34%). High-throughput sequencing revealed that nitrogen loadings increased the abundance of nitrifying bacteria (e.g., Nitrospira) and reduced the abundance of denitrifying bacteria (e.g., Nakamurella, Thermomonas, and Zoogloea) through linear discriminant analysis (LDA).

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Section: Discussionmentioning

confidence: 99%

Nitrogen loading effects on nitrification and denitrification with functional gene quantity/transcription analysis in biochar packed reactors at 5 °C

Ding

Pan

et al. 2018

Sci Rep

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“…Sediment sampling was performed at the Pangong lake (33°43' 04.59"N:78°53' 48.48"E), Ladakh, J&K, India, to isolate psychrophilic and psychrotolerant bacterial strains as reported by Mishra et al [18], Rathour et al [19] and Khosla et al [20]. During winters, this lake stays completely frozen [21].…”

Section: Isolation and Screening Of The Bacterial Strains For Amylasementioning

confidence: 99%

“…Bacterial culture incubated in amylase-producing medium with 1% soluble starch was harvested at 14,000×g (30 min, 4 °C) in a refrigerated centrifuge. Ammonium sulphate (0-80%) was used to saturate the supernatant with constant stirring at 4 °C, followed by a 20 min centrifugation (14,000×g) [20]. The fraction of ammonium sulphate in a dialysis bag was dialyzed against 0.05 M sodium phosphate buffer (pH 6.9, 4 °C) for 6 h. Thereafter, the concentrated enzyme was packed onto a column of DEAE-cellulose, stabilized with the same buffer.…”

Section: Purification Of α-Amylasementioning

confidence: 99%

Production and characterization of psychrophilic α-amylase from a psychrophilic bacterium, Shewanella sp. ISTPL2

Rathour

Gupta

Tyagi

et al. 2020

Amylase

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A psychrophilic and halophilic bacterial isolate, Shewanella sp. ISTPL2, procured from the pristine Pangong Lake, Ladakh, Jammu and Kashmir, India, was used for the production and characterization of the psychrophilic and alkalophilic α-amylase enzyme. The α-amylase is a critical enzyme that catalyses the hydrolysis of α-1,4-glycosidic bonds of starch molecules and is predominately utilized in biotechnological applications. The highest enzyme activity of partially purified extracellular α-amylase was 10,064.20 U/mL after 12 h of incubation in a shake flask at pH 6.9 and 10 °C. Moreover, the maximum intracellular α-amylase enzyme activity (259.62 U/mL) was also observed at 6 h of incubation. The extracellular α-amylase was refined to the homogeneity with the specific enzyme activity of 36,690.47 U/mg protein corresponding to 6.87-fold purification. The optimized pH and temperature for the α-amylase were found to be pH 8 and 4 °C, respectively, suggesting its stability at alkaline conditions and low or higher temperatures. The amylase activity was highly activated by Cu2+, Fe2+ and Ca2+, while inhibited by Cd2+, Co2+ and Na2+. As per our knowledge, the current study reports the highest activity of a psychrophilic α-amylase enzyme providing prominent biotechnological potential.

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“…One other interesting application of biochars is using them as support for the active phase. This is common for base catalysts, where a basic oxide can be supported but is also valid for immobilization of proper enzymes like lipase onto biochar surface [33]. Activation with bases: Treatment with a base such as NaOH or KOH has been commonly adopted to increase the surface area and porosity of biochar [27][28][29].…”

Section: Biodiesel Formation Through Transesterification Reaction Usimentioning

confidence: 99%

Section: Biodiesel Formation Through Transesterification Reaction Usimentioning

confidence: 99%

Biochars and Their Use as Transesterification Catalysts for Biodiesel Production: A Short Review

Vakros

2018

Catalysts

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Biodiesel can be a significant alternative for diesel. Usually, it is produced through transesterification with a base catalyst. Using heterogeneous catalysts for transesterification, the process can be more efficient. Among the possible catalysts that can be used, biochars combine high performance for transesterification and valorization of waste biomass. Biochars are cheap materials, and are easy to activate through chemical treatment with acid or base solutions. In this short review, the application of biochar as solid heterogeneous catalysts for transesterification of lipids to produce biodiesel is discussed.

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Biodiesel production from lipid of carbon dioxide sequestrating bacterium and lipase of psychrotolerant Pseudomonas sp. ISTPL3 immobilized on biochar

Cited by 64 publications

References 46 publications

Nitrogen loading effects on nitrification and denitrification with functional gene quantity/transcription analysis in biochar packed reactors at 5 °C

Nitrogen loading effects on nitrification and denitrification with functional gene quantity/transcription analysis in biochar packed reactors at 5 °C

Production and characterization of psychrophilic α-amylase from a psychrophilic bacterium, Shewanella sp. ISTPL2

Biochars and Their Use as Transesterification Catalysts for Biodiesel Production: A Short Review

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