Biodiesel Production from Transesterification with Lipase from Pseudomonas cepacia Immobilized on Modified Structured Metal Organic Materials

Abstract

This research presents the modification of MOF-199 and ZIF-8 using furfuryl alcohol (FA) as a carbon source to subsequently fix lipase from Pseudomonas cepacia and use these biocatalysts in the transesterification of African palm oil (APO). The need to overcome the disadvantages of free lipases in the biodiesel production process led to the use of metal organic framework (MOF)-type supports because they provide greater thermal stability and separation of the catalytic phase, thus improving the activity and efficiency in relation to the use of free lipase, disadvantages that could not be overcome with the use of other types of catalysts used in transesterification/esterification reactions for the production of biodiesel. The modification of MOFs ZIF-8 and MOF-199 with FA increases the pore volume which allows better immobilization of Pseudomonas cepacia lipase (PCL). The results show that these biocatalysts undergo transesterification with biodiesel yields above 90%. Additionally, studies were carried out on the effect of (1) enzyme loading, 2) enzyme immobilization time, (3) enzyme immobilization temperature, and (4) pH on the % immobilization of the enzyme and the specific activity. The results show that the highest immobilization efficiency for the FA@ZIF-8 support has a value of 91.2% when the load of this support was 3.5 mg/mg and has a specific activity of 142.5 U/g protein. The FA@MOF-199 support presented 80.3% enzyme immobilization and 125% U/g specific activity protein. We established that the specific activity increases in the period from 0.5 to 5.0 h for the systems under investigation. After this time, both the specific activity and the % efficiency of enzyme immobilization decrease. Therefore, 5.0 h (immobilization efficiency of 95 and 85% for FA@MOF-199, respectively) was chosen as the most appropriate time for PCL immobilization. Methods of adding methanol, with three and four steps, were tested, where biodiesel yields greater than 90% were obtained for the biocatalysts synthesized in this

Alloyed Trimetallic Nanocomposite as an Efficient and Recyclable Solid Matrix for Ideonella sakaiensis Lipase Immobilization

“…Enzyme immobilization therefore has been explored as an effective tool for eliminating these hindrances and as a general strategy for improving activity and stability. A variety of solid supports, as a matrix to which an enzyme strongly adheres, has been extensively explored as a potentially reliable solution to the problem of stability and recoverability. ,− Though significant progress has been made in the field of enzyme immobilization, the need to explore other solid support matrices with hitherto unknown structural architectures and functional features or a modification of existing structures, which could ensure effective enzyme binding, allow easy diffusion of substrate and products, hinder mass diffusion limitation, and provide broader stability to the enzyme, continues to be a subject of research importance. , …”