Biodiesel Production from Waste Palm Cooking Oil Using Immobilized Candida rugosa Lipase

Abdulla, Rahmath; Derman, Eryati; K.Mathialagan, Thivyasri; Yaser, Abu Zahrim; Samah, Mohd Armi Abu; Gansau, Jualang Azlan; Najmuddin, Syed Umar Faruq Syed

doi:10.3390/su142013632

Cited by 3 publications

(1 citation statement)

References 65 publications

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“…Table 4 shows the biodiesel yields obtained for the transesterification of the waste cooking oil under different reaction conditions. When compared with the results obtained by other authors, the yield values observed here are promising, since 90% was obtained using the fungal enzyme extract, a result which is similar to that achieved in studies that used purified enzymes for the biocatalytic reaction [54,82,88,89].…”

Section: Raw Materials Reaction Conditions (T; A:o; T; A) Biocatalyst...supporting

confidence: 88%

Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica

Rodrigues,

Cardoso,

Santos

et al. 2023

Energies

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The demand for biodiesel worldwide is skyrocketing as the need to replace fossil diesel with renewable energy sources becomes increasingly pressing. In this context, biocatalysis is emerging as an environmentally friendly and highly efficient alternative to chemical catalysis. When combined with the utilization of waste materials, it has the potential to make the process of biodiesel production sustainable. In the study, the potential of an extract rich in lipase produced by an Amazonian endophytic fungus as a biocatalyst in the transesterification of waste cooking oil for biodiesel production has been systematically investigated. The fungus Endomelanconiopsis endophytica exhibited an enzyme production of 11,262 U/mL after 120 h of cultivation. The lipolytic extract demonstrated its highest catalytic activity at 40 °C and a pH of 5.5. Using soybean oil and frying residue as raw materials, biodiesel was produced through biocatalytic transesterification, and yields of 91% and 89% (wt.), respectively, were achieved. By evaluating the process parameters, a maximum biodiesel yield of 90% was achieved using ethanol at a ratio of 3:1 ratio within 120 min. The experimental results demonstrate the feasibility and sustainability of applying a fungal enzymatic extract as a biocatalyst in the production of ethyl esters using waste cooking oil as a raw material.

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Section: Raw Materials Reaction Conditions (T; A:o; T; A) Biocatalyst...supporting

confidence: 88%

Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica

Rodrigues,

Cardoso,

Santos

et al. 2023

Energies

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Valorization of fish waste for sustainable biofuel production: A case study in Ghana

Akonnor Osei,

Tulashie,

Opoku Boateng

et al. 2024

Biofuels

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Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

Zhao,

Han,

Zhou

et al. 2024

Molecules

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Developing reusable and easy-to-operate biocatalysts is of significant interest in biodiesel production. Here, magnetic whole-cell catalysts constructed through immobilizing recombinant Escherichia coli cells (containing MAS1 lipase) into Fe3O4–chitosan magnetic microspheres (termed MWCC@MAS1) were used for fatty acid methyl ester (FAME) production from waste cooking oil (WCO). During the preparation process of immobilized cells, the effects of chitosan concentration and cell concentration on their activity and activity recovery were investigated. Optimal immobilization was achieved with 3% (w/v) chitosan solution and 10 mg wet cell/mL cell suspension. Magnetic immobilization endowed the whole-cell catalysts with superparamagnetism and improved their methanol tolerance, enhancing the recyclability of the biocatalysts. Additionally, we studied the effects of catalyst loading, water content, methanol content, and reaction temperature on FAME yield, optimizing these parameters using response surface methodology and Box–Behnken design. An experimental FAME yield of 89.19% was gained under the optimized conditions (3.9 wt% catalyst loading, 22.3% (v/w) water content, 23.0% (v/w) methanol content, and 32 °C) for 48 h. MWCC@MAS1 demonstrated superior recyclability compared to its whole-cell form, maintaining about 86% of its initial productivity after 10 cycles, whereas the whole-cell form lost nearly half after just five cycles. These results suggest that MWCC@MAS1 has great potential for the industrial production of biodiesel.

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Biodiesel Production from Waste Palm Cooking Oil Using Immobilized Candida rugosa Lipase

Cited by 3 publications

References 65 publications

Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica

Biocatalyzed Transesterification of Waste Cooking Oil for Biodiesel Production Using Lipase from the Amazonian Fungus Endomelanconiopsis endophytica

Valorization of fish waste for sustainable biofuel production: A case study in Ghana

Biodiesel Production from Waste Cooking Oil Using Recombinant Escherichia coli Cells Immobilized into Fe3O4–Chitosan Magnetic Microspheres

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