Biodiesel production using enzymatic transesterification – Current state and perspectives

Gog, Adriana; Roman, Marius; Toşa, Monica Ioana; Paizs, Csaba; Irimie, Florin Dan

doi:10.1016/j.renene.2011.08.007

Cited by 374 publications

(237 citation statements)

References 108 publications

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“…However, fish processing waste can also be converted into proteins for human consumption [9][10][11][12] and oil for bio-energy [13][14][15][16]. The work in this study involves a laboratory scale enzymatic hydrolysis of whole mackerel fish and fish waste (frames, head, fin, tail, skin and gut) for the production of protein and further hydrolysis to amino acids.…”

Section: Introductionmentioning

confidence: 99%

Extraction of Proteins from Mackerel Fish Processing Waste Using Alcalase Enzyme

Vv¹,

Ae²,

Ms³

et al. 2013

J Bioproces Biotechniq

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Fish proteins are found in the flesh, head, frames, fin, tail, skin and guts of the fish in varying quantities. Unutilized fish and fish processing waste can be used to produce fish proteins which contain amino acids and many bioactive peptides. After removing the fish flesh during the fish processing operation, all other parts are considered wastes which are not properly utilized. The aim of this study was to evaluate the enzymatic extraction of protein from mackerel fish processing waste. Enzymatic extraction of proteins was carried out using alcalase enzyme at three concentrations (0.5, 1 or 2%) and four hydrolysis times (1, 2, 3 and 4 h). The fish protein hydrolysate was dried using a spray dryer to obtain protein powder. The highest protein yield (76.30% from whole fish and 74.53% from the frame) was obtained using 2.0% enzyme concentration after 4 h of hydrolysis. The results showed that increasing the enzyme concentration from 0.5 to 2% (400%) increased the protein yield by 3.13-43.52% depending upon the fish part and reaction time used. Increasing the enzyme concentration by 4 fold for a small increase in protein yield may appear unjustified. Therefore, the enzyme concentration of 0.5% should be used for the protein extraction unless the enzyme is recycled or an immobilized reactor is used in order to reduce the cost associated with the enzyme. Also, increasing the hydrolysis time from 1 to 4 h (400%) increased the protein yield by 16.45 -50.82% depending upon the fish part and enzyme concentration used. Increasing the hydrolysis time by 4 fold for a small increase in protein yield will increase the capital and operating costs of protein production. A shorter hydrolysis time will allow more throughput and/or reduce the volume of the reactor thereby reducing the cost of protein extraction. Therefore, a 1 h reaction time for protein extraction is recommended. The results showed that the combined fish waste can be used for protein extraction without any segregation.

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Section: Introductionmentioning

confidence: 99%

Extraction of Proteins from Mackerel Fish Processing Waste Using Alcalase Enzyme

Vv¹,

Ae²,

Ms³

et al. 2013

J Bioproces Biotechniq

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“…At 50 , the DE of RA was higher than 94 at 4 h, which may be attributed to the fact that Novozym 435 has shown to be stable at 50-60 32 . However, when reaction temperature was above 50 , the DE of RA and the initial reaction rate significantly decreased, which might be ascribed to the thermal denaturation of the lipase to decrease of enzyme stability and the volatilization of methanol 33 . To obtain good reaction rate and product yield, reaction temperature of 50 was chosen in the following experiments.…”

Section: Screening Of Lipasesmentioning

confidence: 98%

Enhanced Enzymatic Preparation of Biodiesel Using Ricinoleic Acid as Acyl Donor: Optimization Using Response Surface Methodology

Wang

Sun

2016

J. Oleo Sci.

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“…The published version will differ from this one as a result of linguistic and technical corrections and layout editing. Selected strains of Mucor miehei, Rhizopus oryzae, Candida antarctica and Pseudomonas cepacia are regarded as major producing organisms for production of lipases (49). The main problem for using soluble enzymes lies in fact that they are usually delivered in stabilized water solutions (with added preservative for the inhibition of microbial growth, e.g., benzoate and with stabilizer to prevent enzyme denaturation e.g.…”

Section: Enzymatic Catalytic Transesterificationmentioning

confidence: 99%

Recent Trends in Biodiesel and Biogas Production

Bušić

Kundas

Morzak

et al. 2018

Food Technol. Biotechnol.

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SummaryBiodiesel and biogas are two very important sources of renewable energy worldwide, and particularly in EU countries. While biodiesel is almost exclusively used as transportation fuel, biogas is mostly used for production of electricity and heat. The application of more sophisticated purification techniques in production of pure biomethane from biogas allows its delivery to natural gas grid and its subsequent use as transportation fuel. While biogas is produced mostly from waste materials (landfills, manure, sludge from wastewater treatment, agricultural waste), biodiesel in EU is mostly produced from rapeseed or other oil crops that are used as food. This raises the 'food or fuel' concerns. To mitigate this problem considerable efforts have been done to use non-food feedstock for biodiesel production. These include all kinds of waste oils and fats, but recently more attention has been devoted to production of microbial oils by cultivation of microorganisms that are able to accumulate high amounts of lipids in their biomass. Promising candidates for microbial lipids production can be found www.ftb.com.hrPlease note that this is an unedited version of the manuscript that has been accepted for publication. This version will undergo copyediting and typesetting before its final form for publication. We are providing this version as a service to our readers. The published version will differ from this one as a result of linguistic and technical corrections and layout editing.2 among different strains of filamentous fungi, yeast, bacteria and microalgae. Feedstocks of interest are agricultural waste rich in carbohydrates as well as different lignocellulosic raw materials where some technical issues have to be resolved. In this work, recovery and purification of biodiesel and biogas were also considered.

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Biodiesel production using enzymatic transesterification – Current state and perspectives

Cited by 374 publications

References 108 publications

Extraction of Proteins from Mackerel Fish Processing Waste Using Alcalase Enzyme

Extraction of Proteins from Mackerel Fish Processing Waste Using Alcalase Enzyme

Enhanced Enzymatic Preparation of Biodiesel Using Ricinoleic Acid as Acyl Donor: Optimization Using Response Surface Methodology

Recent Trends in Biodiesel and Biogas Production

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