Biodiesel Production Using Palm Oil with a MOF-Lipase B Biocatalyst from Candida Antarctica: A Kinetic and Thermodynamic Study

Abstract

This research presents the results of the immobilization of Candida Antarctica Lipase B (CALB) on MOF-199 and ZIF-8 and its use in the production of biodiesel through the transesterification reaction using African Palm Oil (APO). The results show that the highest adsorption capacity, the 26.9 mg·g−1 Lipase, was achieved using ZIF-8 at 45 °C and an initial protein concentration of 1.20 mg·mL−1. The results obtained for the adsorption equilibrium studies allow us to infer that CALB was physically adsorbed on ZIF-8 while chemically adsorbed with MOF-199. It was determined that the adsorption between Lipase and the MOFs under study better fit the Sips isotherm model. The results of the kinetic studies show that adsorption kinetics follow the Elovich model for the two synthesized biocatalysts. This research shows that under the experimental conditions in which the studies were carried out, the adsorption processes are a function of the intraparticle and film diffusion models. According to the results, the prepared biocatalysts showed a high efficiency in the transesterification reaction to produce biodiesel, with methanol as a co-solvent medium. In this work, the catalytic studies for the imidazolate, ZIF-8, presented more catalytic activity when used with CALB. This system presented 95% biodiesel conversion, while the biocatalyst formed by MOF-199 and CALB generated a catalytic conversion percentage of 90%. Although both percentages are high, it should be noted that CALB-MOF-199 presented better reusability, which is due to chemical interactions.

Lipase Immobilization over Hierarchically Carbonized Macro-MIL-88A for Biodiesel Production

“…The PDMS coating significantly mitigated glycerol adsorption affinity over Macro-Fe 3 O 4 -C-TLL-PDMS, leading to a pronounced enhancement in its wettability within the oil system . Furthermore, the observed resistance to deactivation in hydrophilic environments can be attributed to hydrophobic interactions, thus corroborating this finding . This finding highlights the effectiveness of the coating in reducing the mass transfer boundaries and enhancing the catalytic efficiency.…”

Trends in the Use of Lipases: A Systematic Review and Bibliometric Analysis

“…Lipases have essential applications in promoting various biochemical processes in the industry, with an increasing demand for production, for example, for fresh beef monitoring [ 66 ], biodiesel synthesis [ 67 , 68 , 69 , 70 ], and immobilization [ 71 , 72 , 73 ]. The selectivity of lipases contributes to the synthesis of food products that are beneficial to human health, such as oil supplements, omega 3, and omega 6, for example.…”