Biodiesel synthesis from <i>nannochloropsis oculata</i> and <i>chlorella vulgaris</i> through transesterification process using NaOH/zeolite heterogeneous catalyst

Dianursanti,; Sistiafi, A G; Putri, Dwini Normayulisa

doi:10.1088/1755-1315/105/1/012053

Cited by 6 publications

(4 citation statements)

References 17 publications

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“…Indeed, the selected mutant exhibited an increased lipid productivity in +N condition of ~ 80% compared to WT on a volumetric base. This trait is interesting considering the strong increased in C16:0, C16:1, C18:0 and C18:1 without the energetic and economic costs of inducing nutrient starvation, and their possible use for biodiesel production [57,58]. Improved photosynthetic efficiency by manipulating chlorophyll per cell content is thus a suitable strategy to increase lipid productivity in N. gaditana.…”

Section: Discussionmentioning

confidence: 99%

Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants

Cecchin

Berteotti

Paltrinieri

et al. 2020

Biotechnol Biofuels

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Background: Nannochloropsis gaditana is a photosynthetic unicellular microalgae considered one of the most interesting marine algae to produce biofuels and food additive due to its rapid growth rate and high lipid accumulation. Although microalgae are attractive platforms for solar energy bioconversion, the overall efficiency of photosynthesis is reduced due to the steep light gradient in photobioreactors. Moreover, accumulation of lipids in microalgae for biofuels production is usually induced in a two-phase cultivation process by nutrient starvation, with additional time and costs associated. In this work, a biotechnological approach was directed for the isolation of strains with improved light penetration in photobioreactor combined with increased lipids productivity. Results: Mutants of Nannochloropsis gaditana were obtained by chemical mutagenesis and screened for having both a reduced chlorophyll content per cell and increased affinity for Nile red, a fluorescent dye which binds to cellular lipid fraction. Accordingly, one mutant, called e8, was selected and characterized for having a 30% reduction of chlorophyll content per cell and an almost 80% increase of lipid productivity compared to WT in nutrient-replete conditions, with C16:0 and C18:0 fatty acids being more than doubled in the mutant. Whole-genome sequencing revealed mutations in 234 genes in e8 mutant among which there is a non-conservative mutation in the dgd1 synthase gene. This gene encodes for an enzyme involved in the biosynthesis of DGDG, one of the major lipids found in the thylakoid membrane and it is thus involved in chloroplast biogenesis. Lipid biosynthesis is strongly influenced by light availability in several microalgae species, including Nannochloropsis gaditana: reduced chlorophyll content per cell and more homogenous irradiance in photobioreactor is at the base for the increased lipid productivity observed in the e8 mutant. Conclusions: The results herein obtained presents a promising strategy to produce algal biomass enriched in lipid fraction to be used for biofuel and biodiesel production in a single cultivation process, without the additional complexity of the nutrient starvation phase. Genome sequencing and identification of the mutations introduced in e8 mutant suggest possible genes responsible for the observed phenotypes, identifying putative target for future complementation and biotechnological application.

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Section: Discussionmentioning

confidence: 99%

Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants

Cecchin

Berteotti

Paltrinieri

et al. 2020

Biotechnol Biofuels

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“…Microalgae are considered ideal biomass materials for the production of thirdgeneration biofuels due to their faster growth rate, ability to thrive in non-arable terrestrial or aquatic environments, and higher lipid productivity [40]. Lipids obtained from microalgae can be transformed into biodiesel through transesterification reactions using heterogeneous or homogeneous catalysts [41]. Our study's comparative analysis of lipid concentrations in microalgae groups revealed that the highest value was recorded in Chlorella vulgaris, followed by local microalgae (Figure S1).…”

Section: The Likelihood Of Biodiesel Productionmentioning

confidence: 85%

“…Microalgae are considered prominent sources of biofuel due to their higher levels of lipid production, and can be used as raw material for biodiesel production. Sistiafi and Putri [41] noted that biodiesel yields produced from the lipids of Chlorella vulgaris were better than those from Nannochloropsis oculate [41], while Aguoru and Okibe [42] reported that oil extracted from Chlorella vulgaris contained unsaturated and saturated fatty acids, with the most abundant chain lengths being C9:0, 10:0, 13:0, 16:0, 18:0, 8:1, and 18:2, with C18:1 dominating. They also established Chlorella vulgaris.…”

Section: The Likelihood Of Biodiesel Productionmentioning

confidence: 99%

Unexplored Potential: Metabolite Screening of Local Lake Algae Isolated from Al-Asfar Lake in Saudi Arabia

Alsanie,

Dhawi

2023

Water

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In this study, we aimed to explore the commercial potential of various microalgae variants found in Al-Asfar Lake, Saudi Arabia, which have not been extensively investigated previously. We conducted a comprehensive analysis of the metabolic profiles of algae isolated from Al-Asfar Lake. The isolated algae were subjected to molecular analysis using specific primers for the Chlorophyceae class to confirm their identity. Subsequently, we compared the concentration of metabolites in the locally isolated Chlorella vulgaris from Al-Asfar Lake with five commercially available algae (Tetraselmis, Nannochloropsis, Chlorella vulgaris, Spirulina, and Isochrysis). To perform the metabolomics analysis, we employed untargeted ultra-performance liquid chromatography (UPLC) coupled with mass spectrometry (MS) analysis, which yielded a total of 168 metabolites from the microalgae samples. The data were further analyzed using MetaboAnalyst. The results revealed two distinct clusters of microalgae: the first cluster comprised Chlorella vulgaris and the microalgae isolated from the lake, while the second cluster consisted of two sub-clusters, with Isochrysis grouped with Tetraselmis, and Nannochloropsis clustered with Spirulina. Notably, the metabolites of Al-Asfar Lake algae showed a remarkable similarity to Chlorella vulgaris. These findings have significant implications for the environmental aspect of Al-Asfar Lake, shedding light on critical insights into the metabolites and commercial potential of the lake’s microalgae. The valuable insights gained from this research can be utilized to investigate the impact of nutrient abundance on the lake’s biodiversity, enhance microalgal biomass production for biofuel applications, and explore the reuse of lake water in agriculture and environmental restoration projects. Overall, our study provides important groundwork for understanding the potential of Al-Asfar Lake microalgae and their application in various industries, contributing to the sustainable development and environmental health of the region.

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“…The study optimized the transesterification process using response surface methodology (RSM) and achieved a biodiesel yield of 92.03% under optimal conditions [74]. Jazie et al [75] achieved higher yields of biodiesel through in-situ transesterification using dodecylbenzenesulfonic acid as a catalyst, highlighting its efficiency in reducing the cost of biodiesel production. The in-situ transesterification process yielded larger amounts of biodiesel compared to the two-step extraction-transesterification procedure.…”

Section: Transesterification For Biodiesel Production and Catalystsmentioning

confidence: 99%

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

Singh,

Jain,

Dave

et al. 2024

Preprint

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Chlorella vulgaris has considerable promise as a raw material for biofuel production since it has a high productivity per unit area, has a minimum influence on the environment, and does not significantly affect food security. Nevertheless, the low fatty content of their biomass poses a considerable economic obstacle for commercialization. Increasing the amount of biomass and lipids produced by these algae is essential for improving the economic feasibility of using them as biofuel sources. This review highlights the significance of identifying appropriate algal strains, namely those found in local environments, and utilising mutagenesis and genetic engineering techniques to create 'platform strains'. Prior endeavours have primarily concentrated on altering environmental and dietary parameters to enhance the production of biomass and lipids. Here, we review a scientific literature that examines biotechnological approaches and develops methodologies designed to increase lipid production, emphasising the importance of aligning engineering efforts with breakthroughs in DNA manipulation tools. In addition, the review research evaluates the present economic and commercial situation of algal biorefineries, including any related disadvantages. In summary, this thorough research highlights the importance of using creative methods to tackle the intricacies of lipogenesis and enhance the economic viability of producing biofuels from algae.

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Biodiesel synthesis from nannochloropsis oculata and chlorella vulgaris through transesterification process using NaOH/zeolite heterogeneous catalyst

Cited by 6 publications

References 17 publications

Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants

Improved lipid productivity in Nannochloropsis gaditana in nitrogen-replete conditions by selection of pale green mutants

Unexplored Potential: Metabolite Screening of Local Lake Algae Isolated from Al-Asfar Lake in Saudi Arabia

Sustainable Biotechnology Approaches Using Chlorella vulgaris's Biomass and Lipids as Biofuel Feedstock

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