Biodistribution of 89Zr-DFO-labeled avian pathogenic Escherichia coli outer membrane vesicles by PET imaging in chickens

Li, Zhe; Niu, Ling; Wang, Lizhen; Mei, Ting; Wang, Shang; Cheng, Xi; Li, Yuqing; Xing, Feng; Song, Xiangjun; Shao, Ying; Xu, Yuping; Tu, Jing

doi:10.1016/j.psj.2022.102364

Cited by 3 publications

(1 citation statement)

References 40 publications

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“…They demonstrated that 64 Cu-labeled YbT can be incorporated into FyuA-expressing bacteria and their OMVs selectively. Zhe Li et al 18 report a deferoxamine-based 89 Zr-labeling method of avian pathogenic E. coli OMVs.…”

Section: Introductionmentioning

confidence: 99%

Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display

Szöllősi,

Hajdrik,

Tordai

et al. 2023

Sci Rep

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The important roles of bacterial outer membrane vesicles (OMVs) in various diseases and their emergence as a promising platform for vaccine development and targeted drug delivery necessitates the development of imaging techniques suitable for quantifying their biodistribution with high precision. To address this requirement, we aimed to develop an OMV specific radiolabeling technique for positron emission tomography (PET). A novel bacterial strain (E. coli BL21(DE3) ΔnlpI, ΔlpxM) was created for efficient OMV production, and OMVs were characterized using various methods. SpyCatcher was anchored to the OMV outer membrane using autotransporter-based surface display systems. Synthetic SpyTag-NODAGA conjugates were tested for OMV surface binding and 64Cu labeling efficiency. The final labeling protocol shows a radiochemical purity of 100% with a ~ 29% radiolabeling efficiency and excellent serum stability. The in vivo biodistribution of OMVs labeled with 64Cu was determined in mice using PET/MRI imaging which revealed that the biodistribution of radiolabeled OMVs in mice is characteristic of previously reported data with the highest organ uptakes corresponding to the liver and spleen 3, 6, and 12 h following intravenous administration. This novel method can serve as a basis for a general OMV radiolabeling scheme and could be used in vaccine- and drug-carrier development based on bioengineered OMVs.

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Section: Introductionmentioning

confidence: 99%

Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display

Szöllősi,

Hajdrik,

Tordai

et al. 2023

Sci Rep

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Quantitative Biodistribution of OMVs Using SPECT/CT Imaging with HYNIC-Duramycin Radiolabeling

Szöllősi,

Hajdrik,

Tordai

et al. 2024

ACS Omega

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Introduction: Bacterial outer membrane vesicles (OMVs) are emerging as important players in the host− microbiome interaction, while also proving to be a promising platform for vaccine development and targeted drug delivery. The available methods for measuring their biodistribution, however, are limited. We aimed to establish a high-efficiency radiolabeling method for the treatment of OMVs. Methods: 99m Tc-HYNICduramycin was incubated with OMVs isolated from E. coli BL21(DE3) ΔnlpI ΔlpxM. Radiolabeling efficiency (RLE) and radiochemical purity (RCP) were measured with size-exclusion high-performance liquid chromatography. The biodistribution was quantitatively measured in mice using SPECT/CT imaging. Results: RLE was 81.84 ± 2.03% for undiluted OMV suspension and 56.17 ± 2.29% for 100× dilution. Postlabeling purification with a spindesalting column results in 100% radioactivity in the OMV fraction according to HPLC, indicating 100% RCP of the final product. The biodistribution was found to be in line with previous data reported in the literature using other OMV tracking attempts. Conclusions: Our findings illustrate that using HYNIC-duramycin for labeling of the OMVs enhances efficiency and is easily implementable for in vivo imaging studies, significantly improving upon earlier methods.

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The role of bacterial extracellular vesicles in promoting antibiotic resistance

Qu,

Zhang,

Weng

et al. 2024

Critical Reviews in Microbiology

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Biodistribution of 89Zr-DFO-labeled avian pathogenic Escherichia coli outer membrane vesicles by PET imaging in chickens

Cited by 3 publications

References 40 publications

Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display

Molecular imaging of bacterial outer membrane vesicles based on bacterial surface display

Quantitative Biodistribution of OMVs Using SPECT/CT Imaging with HYNIC-Duramycin Radiolabeling

The role of bacterial extracellular vesicles in promoting antibiotic resistance

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