Biodiversity analysis of the unique geothermal microbial ecosystem of the Blue Lagoon (Iceland) using next-generation sequencing (NGS)

Palińska, Katarzyna A.; Vogt, Janina C.; Surosz, Waldemar

doi:10.1007/s10750-017-3349-2

Cited by 14 publications

(6 citation statements)

References 36 publications

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“…ETS-14 showed 100% identity with strain ThrGT9, and ETS-05 99.2% with strain ElfPHct20 [ 88 ]. In this range, among other species, one of those previously studied in great detail is Cyanobacterium aponinum ETS-03, found also in Blue Lagoon in Iceland, at temperatures from 34 to 42 °C [ 89 ]. Leptolyngbyaceae sp.…”

Section: Discussionmentioning

confidence: 99%

Microbiota of the Therapeutic Euganean Thermal Muds with a Focus on the Main Cyanobacteria Species

et al. 2020

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The Euganean Thermal District has been known since Roman times for the therapeutic properties of peloids, obtained from natural clays that have undergone a traditional maturation process. This leads to the growth of a green microbial biofilm with Cyanobacteria and the target species Phormidium sp. ETS-05 as fundamental components for their ability to synthetize anti-inflammatory molecules. Currently, in-depth studies on the microbiota colonizing Euganean peloids, as in general on peloids utilized worldwide, are missing. This is the first characterization of the microbial community of Euganean thermal muds, also investigating the effects of environmental factors on its composition. We analysed 53 muds from 29 sites (Spas) using a polyphasic approach, finding a stable microbiota peculiar to the area. Differences among mud samples mainly depended on two parameters: water temperature and shading of mud maturation plants. In the range 37–47 °C and in the case of irradiance attenuation due to the presence of protective roofs, a statistically significant higher mud Chl a content was detected. Moreover, in these conditions, a characteristic microbial and Cyanobacteria population composition dominated by Phormidium sp. ETS-05 was observed. We also obtained the complete genome sequence of this target species using a mixed sequencing approach based on Illumina and Nanopore sequencing.

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Section: Discussionmentioning

confidence: 99%

Microbiota of the Therapeutic Euganean Thermal Muds with a Focus on the Main Cyanobacteria Species

et al. 2020

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“…Culturable cells related to psychrophilic groups are poorly described in hot environments and the majority of studies have detected these microorganisms through culture-independent genomic methods, which do not allow us to know if they were in fact Kaur et al (2018) obtained sequences related to Arthrobacter from hot spring soil in India with temperatures from 50 to 90 °C through metagenomics. Psychrobacter sequences were obtained from geothermal water samples in Iceland with temperatures around 40 °C through pyrosequencing of 16S rRNA gene (Palinska et al 2018). Sphingomonas members, which could be psychrophilic or mesophilic chemoorganotrophs, were detected in deep-sea hydrothermal field of the Suiyo Seamount using clone library technique (Kato et al 2009).…”

Section: Discussionmentioning

confidence: 99%

Surviving in hot and cold: psychrophiles and thermophiles from Deception Island volcano, Antarctica

et al. 2018

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Polar volcanoes harbor unique conditions of extreme temperature gradients capable of selecting different types of extremophiles. Deception Island is a marine stratovolcano located at Maritime Antarctica that is notable for its pronounced temperature gradients over very short distances, reaching values up to 100 °C in the fumaroles, and subzero temperatures next to the glaciers. Due to these characteristics, Deception can be considered an interesting analogue of extraterrestrial environments. Our main goal in this study was to isolate thermophilic and psychrophilic bacteria from sediments associated with fumaroles and glaciers from two geothermal sites in Deception Island, comprising temperatures between 0 and 98 °C, and to evaluate their survivability to desiccation and UV-C radiation. Our results revealed that culturable thermophiles and psychrophiles were recovered among the extreme temperature gradient in Deception volcano, which indicates that these extremophiles remain alive even when the conditions do not comprise their growth range. The viability of culturable psychrophiles in hyperthermophilic environments is still poorly understood and our work showed the importance of future studies about their survival strategies in high temperatures. Finally, the spore-forming thermophilic isolates which we found have displayed good survival to desiccation and UV-C irradiation, which suggests their potential to be further explored in astrobiological studies.

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“…This tool simplifies executing quality control (QC) on FastQ files by aggregating QC data like quality by position, sequence content, GC content, and adapter content. The raw data were analyzed as described in Papale et al [44] and references therein [50,51,52] using the bioinformatics analysis software MOTHUR (version 1.39.5). All quality checked sequences were taxonomically classified using the reference alignment with 100 iterations and a minimum bootstrap confidence score of 80%, and clustered into (OTUs) at a 97% similarity level.…”

Section: Methodsmentioning

confidence: 99%

“…All quality checked sequences were taxonomically classified using the reference alignment with 100 iterations and a minimum bootstrap confidence score of 80%, and clustered into (OTUs) at a 97% similarity level. Consensus sequences of each OTU were again classified with a minimum consensus confidence threshold of 80% [52].…”

Section: Methodsmentioning

confidence: 99%

Microbial Assemblages in Pressurized Antarctic Brine Pockets (Tarn Flat, Northern Victoria Land): A Hotspot of Biodiversity and Activity

et al. 2019

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Two distinct pressurized hypersaline brine pockets (named TF4 and TF5), separated by a thin ice layer, were detected below an ice-sealed Antarctic lake. Prokaryotic (bacterial and archaeal) diversity, abundances (including virus-like particles) and metabolic profiles were investigated by an integrated approach, including traditional and new-generation methods. Although similar diversity indices were computed for both Bacteria and Archaea, distinct bacterial and archaeal assemblages were observed. Bacteroidetes and Gammaproteobacteria were more abundant in the shallowest brine pocket, TF4, and Deltaproteobacteria, mainly represented by versatile sulphate-reducing bacteria, dominated in the deepest, TF5. The detection of sulphate-reducing bacteria and methanogenic Archaea likely reflects the presence of a distinct synthrophic consortium in TF5. Surprisingly, members assigned to hyperthermophilic Crenarchaeota and Euryarchaeota were common to both brines, indicating that these cold habitats host the most thermally tolerant Archaea. The patterns of microbial communities were different, coherently with the observed microbiological diversity between TF4 and TF5 brines. Both the influence exerted by upward movement of saline brines from a sub-surface anoxic system and the possible occurrence of an ancient ice remnant from the Ross Ice Shelf were the likely main factors shaping the microbial communities.

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Biodiversity analysis of the unique geothermal microbial ecosystem of the Blue Lagoon (Iceland) using next-generation sequencing (NGS)

Cited by 14 publications

References 36 publications

Microbiota of the Therapeutic Euganean Thermal Muds with a Focus on the Main Cyanobacteria Species

Microbiota of the Therapeutic Euganean Thermal Muds with a Focus on the Main Cyanobacteria Species

Surviving in hot and cold: psychrophiles and thermophiles from Deception Island volcano, Antarctica

Microbial Assemblages in Pressurized Antarctic Brine Pockets (Tarn Flat, Northern Victoria Land): A Hotspot of Biodiversity and Activity

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