This work aims at evaluating the antibacterial activity of 14 lactic acid bacteria (LAB) strains isolated from two species of marine algae (Sargassum muticum and Ulva lactuca) against six pathogenic bacteria, including Escherichia coli ATCC 10536, Staphylococcus aureus ATCC 9144, Bacillus subtilis ATCC 6633, Bacillus cereus ATCC 33019, Bacillus sp. CIP 104717, and Salmonella sp. LAB inhibitory capacity was assessed by using agar well diffusion test. Results showed that significant inhibitory zones of 30.33, 23.33, 13, 12, 9, and 8 mm were obtained against E. coli, B. subtilis, B. cereus, Bacillus sp., Salmonella sp. and S. aureus, respectively. These findings underscore the potential use of marine LAB for producing antibacterial substances, suggesting their applications in biotechnological processes targeting food spoilage and human infections.