This study aimed to assess the floristic composition, vegetation structure and species associations of Cashew agro-ecosystems in the North region of Cameroon. The data were collected in 100 m x 20 m transect methods. An analysis of the data collected in the field was conducted around the diversity and richness of the flora, but also the structure of Cashew agro-ecosystems. For the analyzes of vegetation structure, tree density, basal area and biovolume were calculated. The inventory included trees with a dbh greater than or equal to 5 cm on an area of 1 ha per plot. The experimental device installed is a complete random Fisher block with five repetitions. A total of 7849 individuals in 17 families, 20 genera and 38 species were recorded. Species Important value of vegetation cover indicated that Diospyros mespiliformis, Anogeissus leiocarpus, Annona senegalensis and Terminalia laxiflora are the most represented. Combretaceae are the most ecologically important families. Additionally, the grouping of species into four subdivisions showed that the Shannon diversity index varies from 2.18 ± 0.09 -3.47 ± 0.17 bits, Pielou index vary from 0.98 ± 0.01 -0.99 ± 0.01, Simpson's index ranges from 0.70 ± 0.18 -0.93 ± 0.11, density ranges from 102 ± 1.92 -128 ± 6.36 indiv/ha, basal area ranges from 2.02 ± 0.31 -12.68 ± 2.01 m²/ha, biovolume ranges from 11.58 ± 1.29 -68.03 ± 13.21 m 3 /ha. The Sörensen similarity index is very high with floristic affinities greater than or equal to 90%. The diameters and heights structure show a predominance of young individuals for the diameter and height classes on all plots. The most represented species associations are legumes (Arachis hypogeae: 40.54%) and native fruit trees (Vitellaria paradoxa: 15%). These results show the contribution of Cashew agro-ecosystems in biodiversity conservation.