The objective of the study is to examine the impact of audit committee (AC) characteristics on corporate biodiversity disclosure by using the data of Japanese listed firms. For this purpose, the empirical data has been collected from corporate reports. The study develops hypotheses about the relationship between corporate biodiversity disclosure and AC characteristics such as size, independence, gender diversity, independent chair, frequency of meetings, and financial expertise. We use panel regression (fixed effects model) to test the proposed hypotheses. The empirical results depict that the AC size, gender diversity, AC meetings, and financial expertise significantly impact corporate biodiversity disclosure. However, the AC independence and independence of the chair are not significant. The findings of this study may help regulators, policymakers, investors, shareholders, and managers in assessing and monitoring the corporate biodiversity disclosure in light of AC characteristics.