Biodiversity in Ethiopian linseed (Linum usitatissimum L.): molecular characterization of landraces and some wild species

Mhiret, Worku Negash; Heslop‐Harrison, J.

doi:10.1007/s10722-018-0636-3

Cited by 18 publications

(8 citation statements)

References 45 publications

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“…Molecular marker-based diversity is more precise and economic as it allows breeders to select unrelated individuals among thousands of genotypes within a short period of time which in turn reduces field workload by evaluating only unrelated genotypes. Different molecular marker techniques such as RAPD, AFLP, ISSR, SSR and IRAP has been used to assess the genetic diversity of flax germplasm [16][17][18][19][20][21][22]. The availability of a flax reference genome [23] has created the opportunity of discovery and exploitation of SNP markers, which are abundant and well distributed throughout the genome.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of a flax (Linum usitatissimum L.) global collection

2020

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Background A sustainable breeding program requires a minimum level of germplasm diversity to provide varied options for the selection of new breeding lines. To maximize genetic gain of the North Dakota State University (NDSU) flax breeding program, we aimed to increase the genetic diversity of its parental stocks by incorporating diverse genotypes. For this purpose, we analyzed the genetic diversity, linkage disequilibrium, and population sub-structure of 350 globally-distributed flax genotypes with 6200 SNP markers. Results All the genotypes tested clustered into seven sub-populations (P1 to P7) based on the admixture model and the output of neighbor-joining (NJ) tree analysis and principal coordinate analysis were in line with that of structure analysis. The largest sub-population separation arose from a cluster of NDSU/American genotypes with Turkish and Asian genotypes. All sub-populations showed moderate genetic diversity (average H = 0.22 and I = 0.34). The pairwise F st comparison revealed a great degree of divergence ( F st > 0.25) between most of the combinations. A whole collection mantel test showed significant positive correlation (r = 0.30 and p < 0.01) between genetic and geographic distances, whereas it was non-significant for all sub-populations except P4 and P5 (r = 0.251, 0.349 respectively and p < 0.05). In the entire collection, the mean linkage disequilibrium was 0.03 and it decayed to its half maximum within < 21 kb distance. Conclusions To maximize genetic gain, hybridization between NDSU stock (P5) and Asian individuals (P6) are potentially the best option as genetic differentiation between them is highest ( F st > 0.50). In contrast, low genetic differentiation between P5 and P2 may enhance the accumulation of favorable alleles for oil and fiber upon crossing to develop dual purpose varieties. As each sub-population consists of many genotypes, a Neighbor-Joining tree and kinship matrix assist to identify distantly related genotypes. These results also inform genotyping decisions for future association mapping studies to ensure the identification of a sufficient number of molecular markers to tag all linkage blocks.

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Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of a flax (Linum usitatissimum L.) global collection

2020

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“…Molecular marker-based diversity is more precise and economic as it allows breeders to select unrelated individuals among thousands of genotypes within a short period of time which in turn reduces eld workload by evaluating only unrelated genotypes. Different molecular marker techniques such as RAPD, AFLP, ISSR, SSR and IRAP has been used to assess the genetic diversity of ax germplasm (17)(18)(19)(20)(21)(22)(23). The availability of a ax reference genome (24) has created the opportunity of discovery and exploitation of SNP markers, which are abundant and well distributed throughout the genome.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of a Flax (Linum usitatissimum L.) global collection

Hoque

Fiedler

Rahman

2020

Preprint

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Abstract Background A sustainable breeding program requires a minimum level of germplasm diversity to provide varied options for the selection of new breeding lines. To maximize genetic gain of the North Dakota State University (NDSU) flax breeding program, we aimed to increase the genetic diversity of its parental stocks by incorporating diverse genotypes. For this purpose, we analyzed the genetic diversity, linkage disequilibrium, and population sub-structure of 350 globally-distributed flax genotypes with 6,200 SNP markers Results All the genotypes tested clustered into seven sub-populations (P1 to P7) based on the admixture model and the output of neighbor-joining (NJ) tree analysis and principal coordinate analysis were in line with that of structure analysis. The largest sub-population separation arose from a cluster of NDSU/American genotypes with Turkish and Asian genotypes. All sub-populations showed moderate genetic diversity (average H = 0.22 and I = 0.34). The pairwise F st comparison revealed a great degree of divergence ( F st > 0.25) between most of the combinations. A whole collection mantel test showed significant positive correlation (r = 0.30 and p < 0.01) between genetic and geographic distances, whereas it was non-significant for all sub-populations except P4 and P5 (r= 0.251, 0.349 respectively and p < 0.05). In the entire collection, the mean linkage disequilibrium was 0.03 and it decayed to its half maximum within < 21 kb distance. Conclusions To maximize genetic gain, hybridization between NDSU stock (P5) and Asian individuals (P6) are potentially the best option as genetic differentiation between them is highest ( F st > 0.50). In contrast, low genetic differentiation between P5 and P2 may enhance the accumulation of favorable alleles for oil and fiber upon crossing to develop dual purpose varieties. As each sub-population consists of many genotypes, a Neighbor-Joining tree assists to identify distantly related genotypes. These results also inform genotyping decisions for future association mapping studies to ensure the identification of a sufficient number of molecular markers to tag all linkage blocks.

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“…Different marker systems, such as AFLP, RAPD, ISSR, IRAP, SSR, and SNPs, have been employed in linseed specifically for studying the genetic diversity (33,79,195,197,198,202,(220)(221)(222). The genetic diversity studies with sufficiently large germplasm accessions (>100) have been considered in this review.…”

Section: Genetic Diversity In Linseed Germplasm Elucidated By Molecul...mentioning

confidence: 99%

Diversity of Linum genetic resources in global genebanks: from agro-morphological characterisation to novel genomic technologies – a review

et al. 2023

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Linseed or flaxseed is a well-recognized nutritional food with nutraceutical properties owing to high omega-3 fatty acid (α-Linolenic acid), dietary fiber, quality protein, and lignan content. Currently, linseed enjoys the status of a ‘superfood’ and its integration in the food chain as a functional food is evolving continuously as seed constituents are associated with lowering the risk of chronic ailments, such as heart diseases, cancer, diabetes, and rheumatoid arthritis. This crop also receives much attention in the handloom and textile sectors as the world’s coolest fabric linen is made up of its stem fibers which are endowed with unique qualities such as luster, tensile strength, density, bio-degradability, and non-hazardous nature. Worldwide, major linseed growing areas are facing erratic rainfall and temperature patterns affecting flax yield, quality, and response to biotic stresses. Amid such changing climatic regimes and associated future threats, diverse linseed genetic resources would be crucial for developing cultivars with a broad genetic base for sustainable production. Furthermore, linseed is grown across the world in varied agro-climatic conditions; therefore it is vital to develop niche-specific cultivars to cater to diverse needs and keep pace with rising demands globally. Linseed genetic diversity conserved in global genebanks in the form of germplasm collection from natural diversity rich areas is expected to harbor genetic variants and thus form crucial resources for breeding tailored crops to specific culinary and industrial uses. Global genebank collections thus potentially play an important role in supporting sustainable agriculture and food security. Currently, approximately 61,000 germplasm accessions of linseed including 1,127 wild accessions are conserved in genebanks/institutes worldwide. This review analyzes the current status of Linum genetic resources in global genebanks, evaluation for agro-morphological traits, stress tolerance, and nutritional profiling to promote their effective use for sustainable production and nutrition enhancement in our modern diets.

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Biodiversity in Ethiopian linseed (Linum usitatissimum L.): molecular characterization of landraces and some wild species

Cited by 18 publications

References 45 publications

Genetic diversity analysis of a flax (Linum usitatissimum L.) global collection

Genetic diversity analysis of a flax (Linum usitatissimum L.) global collection

Genetic diversity analysis of a Flax (Linum usitatissimum L.) global collection

Diversity of Linum genetic resources in global genebanks: from agro-morphological characterisation to novel genomic technologies – a review

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