Biodiversity of endophytic bacteria and fungi of wild grapes <i>Vitis amurensis</i> Rupr.

Aleynova, Olga A.; Nityagovsky, Nikolay N.; Kiselev, Konstantin V.

doi:10.1051/bioconf/20213905001

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…Studies by Aleynova et al (2021) and Darkazanli and Kiseleva (2020) The same strain of Sphingomonas sp. was also observed to promote the shoot and root length of the soybean plant (Asaf et al, 2017).…”

Section: Identification Of Bacterial Endophytes By 16s Rrna Gene Sequ...mentioning

confidence: 90%

CHARACTERISATION OF ENDOPHYTIC BACTERIA FROM NAM-NAM PLANTS (Cynometra cauliflora) FOR ANTIBACTERIAL ACTIVITY AND PRODUCTION OF PLANT GROWTH PROMOTING FACTORS

KHALIL¹,

Mohamad²,

RAHMAN³

et al. 2022

MAB

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Plant-beneficial microorganisms also known as endophytes colonize the inside healthy tissues of living plants and form mutualistic relationships with them. Endophytes are important components of the plant microbiome and give a variety of benefits to their hosts. Nam-Nam plant (Cynometra cauliflora), is an indigenous tree to Peninsular Malaysia with various medicinal properties. This study aimed to isolate and characterize endophytic bacteria from different parts of Nam-Nam plants such as leaves, stems, and roots. The ethyl acetate extracts from the endophytic bacteria were tested for their antibacterial activity against 7 bacterial pathogens. Plant growth promotion traits including starch hydrolysis, phosphate solubilization, nitrogen fixation, and indole-3 acetic acid (IAA) production were screened among the endophytic bacteria isolates. Molecular identification by 16S rRNA gene sequencing was performed for isolates with good antibacterial activity and plant growth promotion traits. A total of 33 endophytic bacteria comprising 27 Gram-negative and 6 Gram-positive bacteria were isolated. The antibacterial activity was demonstrated by 7 isolates in which R1L3 and TKL2 extracts exhibited significant activity against Bacillus cereus, Escherichia coli, and Proteus vulgaris. Production of IAA was exhibited by 15 isolates wherein R1S4 produced the highest IAA (20.62 µg/mL). Analysis of the 16S gene sequence revealed that RIR2, TKS2/R1L3, and R1S4/R1S5 belonged to Methylobacterium radiotolerans, Mycobacteroides abscessus, and Sphingomonas sp, respectively. The findings from this study showed that Nam-Nam plants harbored endophytic isolates with the potential to be established as a source of natural compounds that can be used to develop new anti-infection agents in the future. This is the first study to report on antibacterial activity and IAA production by endophytic bacteria from the Nam-Nam plants.

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Section: Identification Of Bacterial Endophytes By 16s Rrna Gene Sequ...mentioning

confidence: 90%

CHARACTERISATION OF ENDOPHYTIC BACTERIA FROM NAM-NAM PLANTS (Cynometra cauliflora) FOR ANTIBACTERIAL ACTIVITY AND PRODUCTION OF PLANT GROWTH PROMOTING FACTORS

KHALIL¹,

Mohamad²,

RAHMAN³

et al. 2022

MAB

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“…N. serpens and Nemania spp. have occasionally been isolated as endophytes of cultivated and wild grapes [128,132]. We isolated N. aureolutea (En25-1), a rarely observed species that is closely related to the common N. aenea.…”

Section: Endophyte Diversity and Ecologymentioning

confidence: 99%

Fungal Endophytes: Discovering What Lies within Some of Canada’s Oldest and Most Resilient Grapevines

Ali,

Wright,

Tanney

et al. 2024

JoF

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Plant diseases and pests reduce crop yields, accounting for global crop losses of 30% to 50%. In conventional agricultural production systems, these losses are typically controlled by applying chemical pesticides. However, public pressure is mounting to curtail agrochemical use. In this context, employing beneficial endophytic microorganisms is an increasingly attractive alternative to the use of conventional chemical pesticides in agriculture. A multitude of fungal endophytes are naturally present in plants, producing enzymes, small peptides, and secondary metabolites due to their bioactivity, which can protect hosts from pathogens, pests, and abiotic stresses. The use of beneficial endophytic microorganisms in agriculture is an increasingly attractive alternative to conventional pesticides. The aim of this study was to characterize fungal endophytes isolated from apparently healthy, feral wine grapes in eastern Canada that have grown without agrochemical inputs for decades. Host plants ranged from unknown seedlings to long-lost cultivars not widely propagated since the 1800s. HPLC-MS was used to identify unique endophyte-derived chemical compounds in the host plants, while dual-culture competition assays showed a range in endophytes’ ability to suppress the mycelial growth of Botrytis, which is typically controlled in viticulture with pesticides. Twelve of the most promising fungal endophytes isolated were identified using multilocus sequencing and morphology, while DNA barcoding was employed to identify some of their host vines. These fungal endophyte isolates, which consisted of both known and putative novel strains, belonged to seven genera in six families and five orders of Ascomycota. Exploring the fungal endophytes in these specimens may yield clues to the vines’ survival and lead to the discovery of novel biocontrol agents.

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“…The genus Coniochaeta was also found in abundance (13.37% of Sordariomycetes). Species of this genus are known to be pathogens of woody hosts, including grapevines [49]. There are also some species in this genus that are known to be beneficial in that they are inhibitory to plant pathogenic fungi [49].…”

Section: Effect Of Brassica Cover Crops On Soil Fungal Diversity and ...mentioning

confidence: 99%

Using Brassica Cover Crops as Living Mulch in a Vineyard, Changes over One Growing Season

O’Farrell,

Forge,

Hart

2023

IJPB

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Farmers hoping to manage cropping systems sustainably are turning to cover crops to help mitigate plant pathogens. Plants with biofumigant properties are used to control soil-borne pathogens in agricultural settings, especially in till systems, where the brassicas are incorporated into the soil as green manure or seed meal. The effect of these crops is not well studied in no-till systems; thus, it is hard to know if they are as effective as green manure. Whether or not these cover crops can effect changes during a single growth season has not yet been studied. This study compared the response of the soil microbial community to four different brassica cover crops, two of which are commonly used in vineyards (Sinapis alba L. (white mustard) and Raphanus sativus (L.) Domin (tillage radish)) as well as two brassicas that are native or naturalized to the Okanagan (Capsella bursa-pastoris (L.) Medik. (Shepherd’s purse) and Boechera holboelli (Hornem.) Á. Löve and D. Löve (Holbøll’s rockcress)). Cover crops did not affect fungal species richness, but B. holboelli recover crops were associated with increased evenness among fungal taxa. Both C. bursa-pastoris and S. alba had lower levels of plant parasitic nematodes compared to non-brassica controls. These results were apparent only after a single growing season, which indicates growers could use this approach as needed, minimizing long-term exposure to biofumigants for beneficial soil microbes.

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Biodiversity of endophytic bacteria and fungi of wild grapes Vitis amurensis Rupr.

Cited by 4 publications

References 29 publications

CHARACTERISATION OF ENDOPHYTIC BACTERIA FROM NAM-NAM PLANTS (Cynometra cauliflora) FOR ANTIBACTERIAL ACTIVITY AND PRODUCTION OF PLANT GROWTH PROMOTING FACTORS

CHARACTERISATION OF ENDOPHYTIC BACTERIA FROM NAM-NAM PLANTS (Cynometra cauliflora) FOR ANTIBACTERIAL ACTIVITY AND PRODUCTION OF PLANT GROWTH PROMOTING FACTORS

Fungal Endophytes: Discovering What Lies within Some of Canada’s Oldest and Most Resilient Grapevines

Using Brassica Cover Crops as Living Mulch in a Vineyard, Changes over One Growing Season

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