Biodiversity of Lactobacillus plantarum from traditional Italian wines

Abstract

In this study, 23 samples of traditional wines produced in Southern Italy were subjected to microbiological analyses with the aim to identify and biotype the predominant species of lactic acid bacilli. For this purpose, a multiple approach, consisting in the application of both phenotypic (API 50CHL test) and biomolecular methods (polymerase chain reaction-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing) was used. The results showed that Lactobacillus plantarum was the predominant species, whereas Lb. brevis was detected in lower amount. In detail, out of 80 isolates 58 were ascribable to Lb. plantarum and 22 to Lb. brevis. Randomly amplified polymorphic DNA-polymerase chain reaction was used to highlight intraspecific variability among Lb. plantarum strains. Interestingly, the cluster analysis evidenced a relationship between different biotypes of Lb. plantarum and their origin, in terms of wine variety. Data acquired in this work show the possibility to obtain several malolactic fermentation starter cultures, composed by different Lb. plantarum biotypes, for their proper use in winemaking processes which are distinctive for each wine.

Evaluation of Malolactic Bacteria Associated with Wines from Albariño Variety as Potential Starters: Screening for Quality and Safety

“…Biogeography studies, which show that microbial communities associated with a wine region depend on the environment and the vine variety, corroborate this idea [11]. Moreover, although uncommon, some research has shown the prevalence of malolactic species different from O. oeni in certain wine regions, as well as a growing interest in these to be used as starters given their favorable characteristics [12][13][14]. A recent starter formulation made by Lallemand Inc., based on a selected L. plantarum strain, confirms this evidence.…”

Emerging trends in the application of malolactic fermentation

“…Testa et al (2014) have undertaken a similar study examining the biodiversity of L. plantarum strains from traditional Italian wines. Testa et al (2014) have undertaken a similar study examining the biodiversity of L. plantarum strains from traditional Italian wines.…”

“…As mentioned previously in this review, however, Testa et al (2014) screened L. plantarum strains sourced from different artisanal wineries located in various areas of Southern Italy to select potential L. plantarum strains as candidate starter cultures with the aim of enhancing regional character. As mentioned previously in this review, however, Testa et al (2014) screened L. plantarum strains sourced from different artisanal wineries located in various areas of Southern Italy to select potential L. plantarum strains as candidate starter cultures with the aim of enhancing regional character.…”

“…sation, absence of biogenic amine genes (hdc, tdc and ptc) and malolactic performance resulted in the isolation of two L. plantarum strains, which are to be tested further for performance in a winery. Testa et al (2014) have undertaken a similar study examining the biodiversity of L. plantarum strains from traditional Italian wines. The aim of this work was not only to identify L. plantarum strains suitable for MLF inoculation, but also to select L. plantarum strains as suitable candidates for MLF starter cultures to enhance wine regional identity, as discussed later.…”

“…To date, most regional isolates of malolactic bacteria have focused on O. oeni. As mentioned previously in this review, however, Testa et al (2014) screened L. plantarum strains sourced from different artisanal wineries located in various areas of Southern Italy to select potential L. plantarum strains as candidate starter cultures with the aim of enhancing regional character.…”