In this study gives the characteristic of Salvia officinalis L. genotypes (Lamiaceae)grown in the Southern Steppe of Ukraine. S. officinalis one of the most well-known plants from Salvia L. genus that is used as an aromatic, medicinal, and culinary herb. The plant raw material of this plant is characterized by numerous biological activities that allow use in the pharmacological industry. This plant is characterized by morphological polymorphism and the selection of new genotypes within S. officinalis has a polyfunctional meaning. The plant material of this study was the genotypes of S. officinalis (108-14, 109-14, 108-14-1, 108-14-2, and 113-16). It investigated 3d years of living plants at the flowering stage from the experimental collections of aromatic and medicinal plants in the Kherson region, v. Plodove (Institute of Climate Smart Agriculture of the National Academy of Agrarian Sciences of Ukraine). The height of plants of investigated genotypes was 65.02-86.37 cm, the diameter of the plant was 89.37-117.03 cm, the leaf length was 8.20-11.06 cm, the leaf width was 2.95-3.79 cm, the inflorescence length was 14.52-26.01 cm, the inflorescence diameter was 4.07-5.57 cm, the number of whorls in inflorescences was 8.12-10.55, and the number of flowers in the whorl was 10.02-12.14. The color of the leaf surface, the character of the leaf surface (wrinkles), and colour of the flowers depended on genotype. The mass of raw from the shrub was determined from 350.11 to 560.27 g and the average mass of essential oil of one plant was from 1.37 to 2.01 g depending on genotype. The essential oil content in the herb, leaves, and inflorescences on dry mass was 1.0-1.5%, 1.09-1.79%, and 0.98-1.28%, respectively. In the herbs and leaves, minimal and maximal content of oil were found in genotypes 113-16 and 109-14, respectively. These results can be useful for further selective work within S. officinalis species and including other species of this genus.