Bioelectrical impedance analysis for the estimation of body composition in rats

Rutter, Kerry-Lee; Hennoste, L.; Ward, Leigh C.; Cornish, Bruce; Thomas, Brian

doi:10.1258/002367798780559356

Cited by 30 publications

(29 citation statements)

References 22 publications

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“…Bioelectric impedance was then measured in the frequency range of 4 to 1024 kHz with a multifrequency bioimpedance spectroscopic analyzer (SFB3; UniQuest Ltd., Brisbane, Australia), as described previously (24). In brief, stainless-steel electrodes were inserted 3 to 5 mm subcutaneously.…”

Section: Bioelectric Impedance Measurementsmentioning

confidence: 99%

Decreases in Renal Functional Reserve and Proximal Tubular Fluid Output in Conscious Oophorectomized Rats

Nielsen¹,

Flyvbjerg²,

Bruun³

et al. 2003

Journal of the American Society of Nephrology

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Abstract. Age-dependent glomerulosclerosis with reduced GFR develops earlier among men than among women. Therefore, whether female sex hormones could prevent the agedependent decrease in GFR was investigated. The kidney function in oophorectomized rats treated with placebo (OOX group), estrogen (OOXϩE 2 group), or estrogen plus progesterone (OOXϩE 2 ϩP group) for 5 mo and in sham-operated rats (sham group) was examined. The rats were 13 mo of age at the time of the investigation. They were conscious and chronically instrumented. The results demonstrated that estrogen, alone or in combination with progesterone, was without effect on the baseline GFR and effective renal plasma flow but prevented severe decreases in the renal functional reserve and fractional proximal tubular fluid output (lithium clearance technique, fractional lithium excretion), which were observed in the OOX group. The renal functional reserve (estimated by stimulation with glycine) was Ϫ223 Ϯ 151, 483 Ϯ 129, 675 Ϯ 76, and 208 Ϯ 140 l/min in the OOX, OOXϩE 2 , OOXϩE 2 ϩP, and sham groups, respectively. Fractional lithium excretion was 12.4 Ϯ 3.1, 26.8 Ϯ 2.0, 31.8 Ϯ 2.5, and 23.6 Ϯ 3.3% in the OOX, OOXϩE 2 , OOXϩE 2 ϩP, and sham groups, respectively. In conclusion, oophorectomy at the age of 8 mo did not produce a decrease in baseline GFR in female rats within a period of 5 mo. However, oophorectomy led to severe decreases in the renal functional reserve and fractional proximal tubular fluid output. Both effects were prevented with administration of estrogen. Sham-operated rats demonstrated values for renal functional reserve and fractional lithium excretion that were between those observed for the OOX group and the groups treated with sex hormones.

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Section: Bioelectric Impedance Measurementsmentioning

confidence: 99%

Decreases in Renal Functional Reserve and Proximal Tubular Fluid Output in Conscious Oophorectomized Rats

Nielsen¹,

Flyvbjerg²,

Bruun³

et al. 2003

Journal of the American Society of Nephrology

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“…However, few studies have used this technique in laboratory animals 11,29,30 , so this method needs to be further investigated.…”

Section: Discussionmentioning

confidence: 99%

“…Also, there were no differences among groups fed different diets. Rutter et al 11 noted that BIA could be used to estimate the total body water of control rats, although the method was less accurate when the procedure was used in rats fed a high-fat diet.…”

Section: Discussionmentioning

confidence: 99%

“…Interest in Bioelectrical Impedance (BIA) has increased recently since it is a noninvasive, inexpensive, fast and reproducible method 9 that provides a good estimate of body composition 10 . Moreover, it can be used repeatedly on the same animal causing minimal disturbance 1,11 .…”

Section: Introductionmentioning

confidence: 99%

“…Studies considering this information for the use of BIA in animals have shown that BIA can be used for predicting the total body water of animals, but there are no specific formulas that distinguish lean from fat mass 1,11,15 . Furthermore, few studies have used this method in rats and no study has investigated whether diet affects body composition and whether BIA would be capable of detecting subtle changes.…”

Section: Introductionmentioning

confidence: 99%

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Bioelectrical impedance analysis and anthropometry for the determination of body composition in rats: effects of high-fat and high-sucrose diets

et al. 2012

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OBJECTIVE: The aim of the present study was to determine the impedance of Wistar rats treated with high-fat and high-sucrose diets and correlate their biochemical and anthropometric parameters with chemical analysis of the carcass. METHODS: Twenty-four male Wistar rats were fed a standard (AIN-93), high-fat (50% fat) or high-sucrose (59% of sucrose) diet for 4 weeks. Abdominal and thoracic circumference and body length were measured. Bioelectrical impedance analysis was used to determine resistance and reactance. Final body composition was determined by chemical analysis. RESULTS: Higher fat intake led to a high percentage of liver fat and cholesterol and low total body water in the High-Fat group, but these changes in the biochemical profile were not reflected by the anthropometric measurements or bioelectrical impedance analysis variables. Anthropometric and bioelectrical impedance analysis changes were not observed in the High-Sucrose group. However, a positive association was found between body fat and three anthropometric variables: body mass index, Lee index and abdominal circumference. CONCLUSION: Bioelectrical impedance analysis did not prove to be sensitive for detecting changes in body composition, but body mass index, Lee index and abdominal circumference can be used for estimating the body composition of rats.

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Co‐Micronized Palmitoylethanolamide and Rutin Associated With Hydroxytyrosol Recover Diabesity‐Induced Hepatic Dysfunction in Mice: In Vitro Insights Into the Synergistic Effect

Melini,

Pirozzi,

Lama

et al. 2024

Phytotherapy Research

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Metabolic dysfunction‐associated fatty liver disease (MAFLD) and diabesity (diabetes related to obesity) are interrelated since glucose and lipid alterations play a vital role in the development of both disorders. Due to their multi‐variant metabolic features, more than one drug or natural product may be required to achieve proper therapeutic effects. This study aimed to evaluate the effectiveness of a formulation containing co‐micronized palmitoylethanolamide and rutin (PEA‐Rut) associated with hydroxytyrosol (HT), namely NORM3, against hepatic damage and metabolic alterations in high‐fat diet (HFD)‐induced diabesity in mice. NORM3 decreased the body weight and fat mass of obese mice. The formulation improved HFD‐altered insulin sensitivity and hepatic glucose production and metabolism, as shown by glucose, insulin, pyruvate tolerance tests, Western blot, and real‐time PCR. In the liver, NORM3 limited macro‐ and micro‐vacuolar steatosis, as revealed by morphological analysis, and reduced the associated hepatic inflammation. NORM3 counteracted lipid dysfunctions of HFD animals, activating AMPK, a key cellular energy sensor, and normalizing the expression of carnitine palmitoyl‐transferase (CPT)1, a rate‐limiting enzyme of fatty acid β‐oxidation, and other genes involved in lipid homeostasis. Relevantly, the hepatic antioxidant activity of NORM3 was proved (reduced ROS and increased detoxifying factors and enzymes). Finally, in vitro synergistic protective effects of the components (PEA‐Rut and HT) on H2O2‐induced oxidative challenge in HepG2 were determined (ROS production, inflammation, and antioxidant defense). Our results show the beneficial effect of NORM3 and its potential as an innovative phytotherapeutic combination in limiting hepatic damage progression and counteracting glucose and lipid dysmetabolism associated with diabesity.

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Bioelectrical impedance analysis for the estimation of body composition in rats

Cited by 30 publications

References 22 publications

Decreases in Renal Functional Reserve and Proximal Tubular Fluid Output in Conscious Oophorectomized Rats

Decreases in Renal Functional Reserve and Proximal Tubular Fluid Output in Conscious Oophorectomized Rats

Bioelectrical impedance analysis and anthropometry for the determination of body composition in rats: effects of high-fat and high-sucrose diets

Co‐Micronized Palmitoylethanolamide and Rutin Associated With Hydroxytyrosol Recover Diabesity‐Induced Hepatic Dysfunction in Mice: In Vitro Insights Into the Synergistic Effect

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