Bioelectrocatalysis of Hemoglobin on Electrodeposited Ag Nanoflowers toward H2O2 Detection

Abstract

Hydrogen peroxide (H2O2) is a partially reduced metabolite of oxygen that exerts a diverse array of physiological and pathological activities in living organisms. Therefore, the accurate quantitative determination of H2O2 is crucial in clinical diagnostics, the food industry, and environmental monitoring. Herein we report the electrosynthesis of silver nanoflowers (AgNFs) on indium tin oxide (ITO) electrodes for direct electron transfer of hemoglobin (Hb) toward the selective quantification of H2O2. After well-ordered and fully-grown AgNFs were created on an ITO substrate by electrodeposition, their morphological and optical properties were analyzed with scanning electron microscopy and UV–Vis spectroscopy. Hb was immobilized on 3-mercaptopropionic acid-coated AgNFs through carbodiimide cross-linking to form an Hb/AgNF/ITO biosensor. Electrochemical measurement and analysis demonstrated that Hb retained its direct electron transfer and electrocatalytic properties and acted as a H2O2 sensor with a detection limit of 0.12 µM and a linear detection range of 0.2 to 3.4 mM in phosphate-buffered saline (PBS). The sensitivity, detection limit, and detection range of the Hb/AgNF/ITO biosensor toward detection H2O2 in human serum was also found to be 0.730 mA mM−1 cm−2, 90 µM, and 0.2 to 2.6 mM, indicating the clinical application for the H2O2 detection of the Hb/AgNF/ITO biosensor. Moreover, interference experiments revealed that the Hb/AgNF/ITO sensor displayed excellent selectivity for H2O2.

Selection of Specific Aptamer against Rivaroxaban and Utilization for Label-Free Electrochemical Aptasensing Using Gold Nanoparticles: First Announcement and Application for Clinical Sample Analysis

“…After this process, the electrodes were then rinsed in ultrapure water and dried in a N 2 stream after this process. Afterward, to obtain an identical working area, a piece of insulating adhesive tape was tightly wrapped around the ITO-PET electrode [ 70 ].…”

An Overview of Environmental Catalysis Mediated by Hydrogen Peroxide

“…In order to ensure an optimized electron transfer, these proteins are often immobilized on highly conductive materials, both organic and inorganic, with a large surface area [248]. Interesting examples of these materials that also find application in the bio-medical sector are gold nanoparticles [249][250][251], nanometric metal oxides [252,253], metal nanoparticle-MOF [254][255][256][257], carbon nanotube-MOF [258] and metal nanoparticle-polymers [259,260].…”

Removal of Thiol-SAM on a Gold Surface for Re-Use of an Interdigitated Chain-Shaped Electrode

“…They have been applied for many applications in energy, biosensor, electronics, and semiconductor fields [ 1 , 2 , 3 ]. There are several deposition techniques, such as electrodeposition for the fabrication of Ni matrix micro- and nano-SiC composite coatings at room and elevated temperature [ 4 ], functionally graded Zn–Ni–Al 2 O 3 coatings [ 5 ], and nickel–fullerene C60 composition coatings [ 6 ]; vacuum thermal evaporation [ 1 , 2 ]; electron beam evaporation [ 7 ]; laser beam evaporation [ 8 ] for depositing thin films; electrochemical deposition for developing thin layers, graphene, and nanoparticles [ 3 , 9 ]; and chemical-absorption for depositing a self-assembled monolayer on a gold substrate [ 10 , 11 , 12 ].…”