Bioelectrocatalytic and electrochemical cascade for phosphate sensing with up to 6 electrons per analyte molecule

Kopiec, Gabriel; Starzec, Karolina; Kochana, Jolanta; Kinnunen-Skidmore, Troy; Schuhmann, Wolfgang; Campbell, Wilbur H.; Ruff, Adrian; Plumeré, Nicolas

doi:10.1016/j.bios.2018.06.047

Cited by 12 publications

(5 citation statements)

References 54 publications

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“…Alternatively, the osmium (Os)-based redox complex easily controls the redox potential by changing ligands [120] and high solubility in water. Therefore, Kopiece et al designed an Os complex with PEGDGE as a crosslinker for the immobilization of purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOx) in order to detect phosphate molecules, as shown in Figure 10d [121]. The PNP and XOx were crosslinked in the redox polymer Os complex (Scheme 4C) via PEGDGE integration, where a 3D support matrix was formed via a PNP/XOx-to-Os complex and PNP-to-XOx through an amineepoxy reaction.…”

Section: Polymeric Matrix/hydrogelmentioning

confidence: 99%

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Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells

Kalyana Sundaram,

Hossain,

Rezki

et al. 2023

Biosensors

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Nanomaterials, including carbon nanotubes, graphene oxide, metal–organic frameworks, metal nanoparticles, and porous carbon, play a crucial role as efficient carriers to enhance enzyme activity through substrate channeling while improving enzyme stability and reusability. However, there are significant debates surrounding aspects such as enzyme orientation, enzyme loading, retention of enzyme activity, and immobilization techniques. Consequently, these subjects have become the focus of intensive research in the realm of multi-enzyme cascade reactions. Researchers have undertaken the challenge of creating functional in vitro multi-enzyme systems, drawing inspiration from natural multi-enzyme processes within living organisms. Substantial progress has been achieved in designing multi-step reactions that harness the synthetic capabilities of various enzymes, particularly in applications such as biomarker detection (e.g., biosensors) and the development of biofuel cells. This review provides an overview of recent developments in concurrent and sequential approaches involving two or more enzymes in sequence. It delves into the intricacies of multi-enzyme cascade reactions conducted on nanostructured electrodes, addressing both the challenges encountered and the innovative solutions devised in this field.

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Section: Polymeric Matrix/hydrogelmentioning

confidence: 99%

“…Scheme 4. Redox polymer employed for enzyme cascade biofuel and biosensor: (A) Fc-LPEI[97,116], (B) cyanoferrate complex[118] (C,D) Os complex[108,121]. Scheme 4.…”

mentioning

confidence: 99%

Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells

Kalyana Sundaram,

Hossain,

Rezki

et al. 2023

Biosensors

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“…In [85], a ZnO nanorod array grown directly on seeded SiO 2 /Si substrate in the gate region of a fieldeffect transistor (FET) and functionalized with pyruvate oxidase (PyO) were employed for phosphate detection in linear range of 0.1 µM to 7.0 mM. Other phosphate biosensors used pyruvate oxidase and a cobalt phthalocyanine screen-printed carbon electrode (CoPC-SPCE) for amperometric phosphate biosensing [86]; or two-enzymes, such as purine nucleoside phosphorylase (PNP) and xanthine oxidase (XOx), to detect phosphates after the several steps cascade oxidation to uric acid [87]. Molecularly Imprinted Polymers, MIPs, obtained from methacrylic acid (MAA) and N-allylthiourea monomers, templated with diphenyl phosphate, triethyl phosphate, and trimethyl phosphate, and deposited on an interdigital capacitive transducer, were reported for phosphate detection in a hydroponic system [88].…”

Section: Total Phosphorous and Phosphatesmentioning

confidence: 99%

Recent Advances in Chemical Sensors for Soil Analysis: A Review

et al. 2022

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The continuously rising interest in chemical sensors’ applications in environmental monitoring, for soil analysis in particular, is owed to the sufficient sensitivity and selectivity of these analytical devices, their low costs, their simple measurement setups, and the possibility to perform online and in-field analyses with them. In this review the recent advances in chemical sensors for soil analysis are summarized. The working principles of chemical sensors involved in soil analysis; their benefits and drawbacks; and select applications of both the single selective sensors and multisensor systems for assessments of main plant nutrition components, pollutants, and other important soil parameters (pH, moisture content, salinity, exhaled gases, etc.) of the past two decades with a focus on the last 5 years (from 2017 to 2021) are overviewed.

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“…A recent study proposed a second generation enzymatic phosphate sensor where the enzymes PNP and XOD are coupled with a redox active osmium complex modified polymer to minimize the oxygen interference. 66 The osmium modified polymer also acts as an immobilization matrix and facilitates the shuttling of electrons from the oxidation reaction to the electrode. The second-generation enzyme sensors provide an alternative to improve the sensor performance by using electrode mediators.…”

Section: Inosine Phosphatementioning

confidence: 99%

Review—Solid State Sensors for Phosphate Detection in Environmental and Medical Diagnostics

Patel

Kruse

Selvaganapathy

2022

J. Electrochem. Soc.

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Phosphorus is required for plants and humans to survive because it is needed for cell signaling, skeletal integrity, energy storage, and metabolism. Phosphorus measurements are performed using colorimetric and electrochemical methods. Colorimetry is the most accepted method for commercial devices while electrochemical systems are still in the research phase. Here we provide the first comprehensive review of solid-state sensors for phosphate monitoring. The review focuses on solid state reagent storage for colorimetric sensors and different materials used in solid state electrochemical sensors. The electrochemical sensors are further classified into three groups: potentiometric, amperometric, and voltammetric. All sensors are evaluated based on parameters such as measurement range, limit of detection (LOD), working pH, and response time. Finally, we discuss limitations of the current sensors and future directions for the development of these sensors.

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Bioelectrocatalytic and electrochemical cascade for phosphate sensing with up to 6 electrons per analyte molecule

Cited by 12 publications

References 54 publications

Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells

Enzyme Cascade Electrode Reactions with Nanomaterials and Their Applicability towards Biosensor and Biofuel Cells

Recent Advances in Chemical Sensors for Soil Analysis: A Review

Review—Solid State Sensors for Phosphate Detection in Environmental and Medical Diagnostics

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