Bioelectrocatalytic hydroxylation of nicotinic acid at an electrode modified with immobilized bacterial cells of Pseudomonas fluorescens in the presence of electron transfer mediators

Takayama, Ken; Kurosaki, Toshiyasu; Ikeda, Tokuji; Nagasawa, Tôru

doi:10.1016/0022-0728(94)03651-i

Cited by 36 publications

(20 citation statements)

References 14 publications

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“…We have, in fact, observed bioelectrocatalytic currents with bacterial cells in solution or immobilized on an electrode using metal complexes or quinone compounds as mediators between the enzymes in the cells and an electrode. The electrochemical method based on mediated bioelectrocatalysis18,50–55 and other electrochemical methods56,57 allowed us to analyze the currents for the catalytic reactions exhibited by whole bacterial cells by a Michaelis‐Menten type equation. The results obtained with several bacterial species are given in Table 1.…”

Section: Quantitative Evaluation Of Catalytic Activities Exhibited Bymentioning

confidence: 99%

1,1,1,3,3,3‐hexafluoroisopropanol induced thermal unfolding and molten globule state of bovine α‐lactalbumin: Calorimetric and spectroscopic studies

Kundu

Kishore

2004

Biopolymers

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The thermal denaturation of alpha-lactalbumin was studied at pH 7.0 and 9.0 in aqueous 1,1,1,3,3,3-hexafluoroisopropanol (HFIP) by high-sensitivity differential scanning calorimetry. The conformation of the protein was analyzed by a combination of fluorescence and circular dichroism measurements. The most obvious effect of HFIP was lowering of the transition temperature with an increase in the concentration of the alcohol up to 0.30M, beyond which no calorimetric transition was observed. Up to 0.30M HFIP the calorimetric and van't Hoff enthalpy remained the same, indicating the validity of the two-state approximation for the thermal unfolding of alpha-lactalbumin. The quantitative thermodynamic parameters accompanying the thermal transitions have been evaluated. Spectroscopic observations confirm that alpha-lactalbumin is in the molten globule state in the presence of 0.50M HFIP at pH 7.0 and 0.75M HFIP at pH 9.0. The results also demonstrate that alpha-lactalbumin in the molten globule state undergoes a noncooperative thermal transition to the denatured state. It is observed that two of four tryptophans are exposed to the solvent in the HFIP induced molten globule state of alpha-lactalbumin compared to four in the 8.5M urea induced denatured state of the protein. It is also observed that the HFIP induced molten globule states at the two pH values are different from the acid induced molten globule state (A state) of alpha-lactalbumin.

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Section: Quantitative Evaluation Of Catalytic Activities Exhibited Bymentioning

confidence: 99%

1,1,1,3,3,3‐hexafluoroisopropanol induced thermal unfolding and molten globule state of bovine α‐lactalbumin: Calorimetric and spectroscopic studies

Kundu

Kishore

2004

Biopolymers

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“…industrius VKM B-1280 were studied by the method of cyclic voltammetry. Peaks of the current corresponding to electrocatalytic process in the system, appearing on a cyclic voltammogram in the presence of biocatalyst and substrate, are a criterion evidencing the possibility in principle to use the compound under study as mediator [12,20]. Figure 1 shows the character of typical changes in cyclic voltammograms for carbon paste electrode modified by 1,1'-dimethylferrocene in the control (Fig.…”

Section: Cyclic Voltammogramsmentioning

confidence: 99%

“…Mediators broaden the range of sensor applications by increasing sensitivity, selectivity, and measurement rate. Biosensors based on bacterial activity and mediators proved to be effective for the monitoring of environmental objects [6,7], detection of glucose and monosaccharides [8,9], ethanol [10,11], and organic acids [12]. Charge acceptance by mediators is used also for development of ecologically safe power sources: microbial biofuel cells [13,14].…”

Section: Introductionmentioning

confidence: 99%

Bioelectrocatalytic Oxidation of Glucose by Immobilized Bacteria Gluconobacter oxydans. Evaluation of Water‐Insoluble Mediator Efficiency

et al. 2006

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For the first time it has been shown possible to use strain G. oxydans sbsp. industrius VKM B-1280 in combination with water-insoluble mediators: ferrocene, 1,1'-dimethylferrocene, 2,5-dibromo-1,4-benzoquinone, and 2-methyl-1,4-benzoquinone in the process of electrocatalytic oxidation of glucose. Cell-mediator interaction has been studied using a carbon paste electrode that comprised a mediator and immobilized bacteria. Catalytic activity of the system "mediator -bacterial cell -substrate" was characterized by three quantities: the maximum reaction rate and the ratios of the Michaelis constant to the distribution constant for the substrate and to that for the electron acceptor. It has been revealed that 2,5-dibromo-1,4-benzoquinone is the most efficient for high catalytic activity.

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“…Use of electron transfer agents other than oxygen (as used in the Clark-type electrode) has a number of advantages [33]. Previously both soluble and insoluble mediators such as p-benzoquinone [34] and ferrocenes [35] have been used in Scheme 1. Reaction of Gibbs reagent with phenol anion to yield an indophenol anion, which is stable under neutral conditions.…”

Section: Introductionmentioning

confidence: 99%

Construction and characterization of phenol-based sensor derived from colloidal chemistry

Bashir

Liu

2009

Sensors and Actuators B: Chemical

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Bioelectrocatalytic hydroxylation of nicotinic acid at an electrode modified with immobilized bacterial cells of Pseudomonas fluorescens in the presence of electron transfer mediators

Cited by 36 publications

References 14 publications

1,1,1,3,3,3‐hexafluoroisopropanol induced thermal unfolding and molten globule state of bovine α‐lactalbumin: Calorimetric and spectroscopic studies

1,1,1,3,3,3‐hexafluoroisopropanol induced thermal unfolding and molten globule state of bovine α‐lactalbumin: Calorimetric and spectroscopic studies

Bioelectrocatalytic Oxidation of Glucose by Immobilized Bacteria Gluconobacter oxydans. Evaluation of Water‐Insoluble Mediator Efficiency

Construction and characterization of phenol-based sensor derived from colloidal chemistry

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