Bioelectrochemical Behavior of the Composite PVP-Os/chitosan as a Mediator with Different Types of Enzymes at Graphite Electrode

Abstract

Chitosan was cross-linked to an osmium redox polymer, poly(4-vinylpyridine) osmium bipyridyl [PVP-Os-(bpy) 2 -Cl], to form PVP-Os-(bpy) 2 -Cl/chitosan composite known to make a porous and hydrophilic film with an enzyme. In this work we demonstrate such a composite is a useful trestle, for hosting various sugars oxidizing enzymes to construct biosensors. Glucose sensing ability has been proven with the following glucose oxidizing redox enzymes; Aspergillus niger glucose oxidase (AnGOX), Myriococcum thermophilum cellobiose dehydrogenase, (MtCDH), glycosylated Agaricus meleagris pyranose dehydrogenase (gAmPDH), fragmented deglycosylated Agaricus meleagris pyranose dehydrogenase (fdgAmPDH), and Aspergillus sp. glucose dehydrogenase (AspGDH), as well as recombinant Glomerella cingulata glucose dehydrogenase (rGcGDH).

Ferrocene Tethered Polyvinyl Alcohol/Silica Film Electrode for Electrocatalytic Sulfite Sensing

“…Polymers modified with redox active moieties like ferrocene, osmium complexes, quinone and viologen derivatives are widely used as a mediators and the immoblization platform for biosensoring and bio-fuel cell applications [7][8][9][10][11][12][13]. Functional polymeric backbones like polyacrylic acid, polyallylamine, polyvinyl alcohol and chitosan are useful for being tethered by redox active centers [14][15][16] and, among them, polyvinyl alcohol is a highly hydrophilic and biocompatible macromolecule.…”

Chitosan-Cu-salen/Carbon Nano-Composite Based Electrode for the Enzyme-less Electrochemical Sensing of Hydrogen Peroxide

“…Chitosan is an amino-polysaccharide prepared by deacetylation of chitin, which is discarded as industrial waste so it can be considered as a low cost and nonhazardous polymer [18,19]. Chitosan bears free amine groups which are used for the immobilization of the metal complexes and redox mediators [20][21][22][23]. Currently, much attention has been focused on developing new nano-materials, which are used for signal amplification in electrochemical methods [24].…”