Bioelectrochemical fuel cell and sensor based on a quinoprotein, alcohol dehydrogenase

Davis, Graham; Hill, H. Allen O.; Aston, William; Higgins, I. J.; Turner, Anthony P. F.

doi:10.1016/0141-0229(83)90013-3

Cited by 79 publications

(48 citation statements)

References 11 publications

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“…Fuel-cell based biosensors first appeared in the literature in the 1980's 21,22 . The first commercial self-powered glucose biosensors were produced by Kyoto Daiichi Kagagu in Japan in 1996 and marketed as the Glucocard™ 23 .…”

Section: Introductionmentioning

confidence: 99%

Cholesterol Self-Powered Biosensor

et al. 2014

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Monitoring the cholesterol level is of great importance, especially for people with high risk of developing heart disease. Here we report on reagentless cholesterol detection in human plasma with a novel single-enzyme, membrane-free, self-powered biosensor, in which both cathodic and anodic bioelectrocatalytic reactions are powered by the same substrate. Cholesterol oxidase was immobilised in a sol-gel matrix on both the cathode and the anode. Hydrogen peroxide, a product of the enzymatic conversion of cholesterol, was electrocatalytically reduced, by the use of Prussian blue, at the cathode. In parallel, cholesterol oxidation catalysed by mediated cholesterol oxidase occurred at the anode. The analytical performance was assessed for both electrode systems separately. The combination of the two electrodes, formed on high surface-area carbon cloth electrodes, resulted in a self-powered biosensor with enhanced sensitivity (26.0 mA M -1 cm -2 ), compared to either of the two individual electrodes, and a dynamic range up to 4.1 mM cholesterol.Reagentless cholesterol detection with both electrochemical systems and with the self-powered biosensor was performed and the results were compared with the standard method of colorimetric cholesterol quantification.

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Section: Introductionmentioning

confidence: 99%

Cholesterol Self-Powered Biosensor

et al. 2014

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“…Direct reduction of artificial electron acceptors (mediators) by free enzymes and intact microbial cells has been used repeatedly in previous years to generate electrical currents in microbial fuel cells (Plotkin et al 1981;Davis et al 1983;Tanaka et al 1983;Delaney et al 1984;Roller et al 1984). Dichlorophenol indophenol (Nishikawa et al 1982), phenazine ethosulphate (Turner et al 1982), and potassium ferricyanide (Ramsey et al 1985) have been applied as mediators in various fuel cell and amperometric systems as possible measures of microbial cell densities.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic oxidation of glycerol by Escherichia coli in an amperometric poised-potential culture system

Emde

Swain

Schink

1989

Appl Microbiol Biotechnol

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Summary. Anaerobic growth of Escherichia coli was studied with glycerol as electron source and a three-electrode poised-potential system with potassium ferricyanide as mediator. Similar to fumarate, potassium ferricyanide was used as electron acceptor in batch-culture experiments. In experiments with regulated electrodes, glycerol was degraded completely, and an electron flow of 3.0 to 4.0 mA was obtained. The electron balances were in the range of 98%-107%, and a growth rate of 0.095 h -1 was calculated. These results are discussed with respect to the energetics of iron reduction by E. coli and by other bacteria.

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“…Prominent among his early studies was the finding that electron transfer between the quinoprotein methanol dehydrogenase and electrodes was proportional to the "fuel" added. The use of low molecular weight redox compounds as the mediator of electron transfer provided a powerful expedient to effect the fast coupling between catalytic activity and current flow [10][11][12]. These findings led to the essential conversion of a fuel cell to an alcohol sensor, which thereby acted as, an important milestone in the development of biosensors.…”

Section: Contribution To the Biosensor Fieldmentioning

confidence: 99%

“…His book "Biosensors: Fundamentals and Applications", as previously mentioned, is still among the most cited works in the biosensor field [9]. His three articles dedicated to "bio-electrochemical fuel cells" published in 1982 and 1983 are generally regarded as laying the foundations for "mediated amperometric biosensors" [10][11][12]. His definitive paper that led to a pletehora of publications involving the use of ferrocene and its derivatives in mediated amperometric biosensors was published by Analytical Chemistry in 1984 [13] and has been cited nearly 1,000 times.…”

Section: Contribution To the Biosensor Fieldmentioning

confidence: 99%

Dedication And Prominence: 31 Years In Biosensors And Bioelectronics Dedicated To Professor Anthony P.F. Turner‘ s 61st Birthday

Tiwari

2011

Adv. Mater. Lett.

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The name Anthony P. F. Turner, biosensor pioneer, is often considered synonymous with his chosen field. This 5th June will be his 61st birthday. We track here his professional footprint, in order to celebrate his upcoming birthday and to commemorate his 31-years of dedication to biosensors. Commemorating this pioneer"s achievements is a multidisciplinary celebration of his prominent contribution to biotechnology, chemistry, biomaterials and nanotechnology.

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Bioelectrochemical fuel cell and sensor based on a quinoprotein, alcohol dehydrogenase

Cited by 79 publications

References 11 publications

Cholesterol Self-Powered Biosensor

Cholesterol Self-Powered Biosensor

Anaerobic oxidation of glycerol by Escherichia coli in an amperometric poised-potential culture system

Dedication And Prominence: 31 Years In Biosensors And Bioelectronics Dedicated To Professor Anthony P.F. Turner‘ s 61st Birthday

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