Bioelectrochemical platform with human monooxygenases: FMO1 and CYP3A4 tandem reactions with phorate

Cheropkina, Hanna; Catucci, Gianluca; Cesano, Federico; Marucco, Arianna; Gilardi, Gianfranco; Sadeghi, Sheila J.

doi:10.1016/j.bioelechem.2022.108327

Cited by 6 publications

(2 citation statements)

References 42 publications

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“…The aim of this work is to develop approaches to improve the efficiency of cytochrome P450 electrocatalysis and the functioning of cytochrome P450 electrodes using natural proteins for obtaining suitable cavities as host frames for enzyme immobilization. From this point of view, we propose an approach that makes it possible to mimic the cellular environment for the creation of a more developed surface with protein cavities for the effective immobilization of the CYP3A4 enzyme based on hybrid biomembranes in the lipid-like bilayer of the electrode modifier didodecyldimethylammonium bromide (DDAB) using the pore-forming protein streptolysin O (SLO) [ 25 , 26 , 27 , 28 , 29 ]. This approach enhances the efficiency of electrochemical CYP3A4-mediated substrate conversion by 297% in the reaction of the N-demethylation of the macrolide antibiotic erythromycin as a well-known CYP3A4 substrate.…”

Section: Introductionmentioning

confidence: 99%

Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs

et al. 2023

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In the present work, screen-printed electrodes (SPE) modified with a synthetic surfactant, didodecyldimethylammonium bromide (DDAB) and streptolysin O (SLO) were prepared for cytochrome P450 3A4 (CYP3A4) immobilization, direct non-catalytic and catalytic electrochemistry. The immobilized CYP3A4 demonstrated a pair of redox peaks with a formal potential of −0.325 ± 0.024 V (vs. the Ag/AgCl reference electrode). The electron transfer process showed a surface-controlled mechanism (“protein film voltammetry”) with an electron transfer rate constant (ks) of 0.203 ± 0.038 s−1. Electrochemical CYP3A4-mediated reaction of N-demethylation of erythromycin was explored with the following parameters: an applied potential of −0.5 V and a duration time of 20 min. The system with DDAB/SLO as the electrode modifier showed conversion of erythromycin with an efficiency higher than the electrode modified with DDAB only. Confining CYP3A4 inside the protein frame of SLO accelerated the enzymatic reaction. The increases in product formation in the reaction of the electrochemical N-demethylation of erythromycin for SPE/DDAB/CYP3A4 and SPE/DDAB/SLO/CYP3A4 were equal to 100 ± 22% and 297 ± 7%, respectively. As revealed by AFM images, the SPE/DDAB/SLO possessed a more developed surface with protein cavities in comparison with SPE/DDAB for the effective immobilization of the CYP3A4 enzyme.

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Section: Introductionmentioning

confidence: 99%

Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs

et al. 2023

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“…However, the presence of polar functionalities by interrupting the aromatic domains makes GO hydrophilic and an electrical insulator [ 16 ]. Interestingly, GO nanosheets can be finely dispersed in polar solvents [ 17 ]. More interestingly, GO can be thermally or chemically reduced (RGO) to reconstruct the hexagonal lattice for restoring graphene’s properties, including electrical conductivity [ 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Functional Piezoresistive Polymer Composites Based on CO2 Laser-Irradiated Graphene Oxide-Loaded Polyurethane: Morphology, Structure, Electrical and Piezoresistive Properties

Mastropasqua

Veca²,

Damin

et al. 2022

Nanomaterials

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Nanocomposite materials have recently attracted great attention for their wide range of applications, such as in smart materials, flexible electronics, and deformation sensing applications. Such materials make it possible to combine a polymer with functional fillers. In this study, flexible artificial leathers, exhibiting insulating properties and containing 1.5 or 2wt.% of graphene oxide (GO) in the polyurethane (PU) layer, were electrically activated via CO2 laser irradiation to obtain conductive paths at the surface exposed to the laser beam. As the material retained its insulating properties out of the irradiation areas, the laser scribing method allowed, at least in principle, a printed circuit to be easily and quickly fabricated. Combining a variety of investigation methods, including scanning electron microscopy (SEM), optical profilometry, IR and Raman spectroscopies, and direct current (DC) and alternate current (AC) electrical measurements, the effects of the laser irradiation were investigated, and the so-obtained electrical properties of laser-activated GO/PU regions were elucidated to unveil their potential use in both static and dynamic mechanical conditions. In more detail, it was shown that under appropriate CO2 laser irradiation, GO sheets into the GO/PU layer were locally photoreduced to form reduced-GO (RGO) sheets. It was verified that the RGO sheets were entangled, forming an accumulation path on the surface directly exposed to the laser beam. As the laser process was performed along regular paths, these RGO sheets formed electrically conductive wires, which exhibited piezoresistive properties when exposed to mechanical deformations. It was also verified that such piezoresistive paths showed good reproducibility when subjected to small flexural stresses during cyclic testing conditions. In brief, laser-activated GO/PU artificial leathers may represent a new generation of metal-free materials for electrical transport applications of low-current signals and embedded deformation sensors.

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Electrochemical Assessment of CYP3A4 Catalytic Activity in Bactosomes

Koroleva,

Kuzikov,

Gilep

et al. 2024

BioNanoSci.

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Bioelectrochemical platform with human monooxygenases: FMO1 and CYP3A4 tandem reactions with phorate

Cited by 6 publications

References 42 publications

Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs

Improving the Efficiency of Electrocatalysis of Cytochrome P450 3A4 by Modifying the Electrode with Membrane Protein Streptolysin O for Studying the Metabolic Transformations of Drugs

Functional Piezoresistive Polymer Composites Based on CO2 Laser-Irradiated Graphene Oxide-Loaded Polyurethane: Morphology, Structure, Electrical and Piezoresistive Properties

Electrochemical Assessment of CYP3A4 Catalytic Activity in Bactosomes

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