Bioelectroremediation of hexadecane in electrical cells containing Aspergillus niger immobilized in alginate

Saavedra, Jayson Baldera; Pérez, Lisbet Judith Noriega; Quiñones, Claudio; Monzón, José Alfredo Cruz; Butrón, Fernando Javier Hurtado; Mantilla, Mario Esparza

doi:10.4136/ambi-agua.2805

Cited by 2 publications

(2 citation statements)

References 22 publications

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“…Morphological characteristics were determined using wet mount microscopy and fluorescence staining with calcein AM (Sigma Aldrich, USA) using a fluorescence microscope (ZEISS, AXIOPHOT) from a 24-hour pure culture growth (Maza et al, 2020). The isolated yeast culture was identified by molecular analysis using polymerase chain reaction (PCR) of the ITS1 region, 5.8S rRNA subunit and ITS2, following the methodology described by Saavedra et al (2022). DNA was extracted from a 48-hour pure culture growth (Nucleic Acid Extraction and Purification Kit, innuPREP DNA Kit, AnalytikJena).…”

Section: Phenotypic and Molecular Characterizationmentioning

confidence: 99%

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

Baldera-Saavedra,

Quiñones-Cerna,

Sánchez-Vásquez

et al. 2024

EREM

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Phenolic compounds have gained international interest due to their carcinogenic, toxic, and bioaccumulative properties, causing adverse effects in both animals and humans. As a result, there is a growing interest in finding alternative and eco-friendly treatment routes for phenol by exploring new microbial cultures with potential adaptation and biodegradation capabilities. In this study, the phenol removal efficiency of Yarrowia lipolytica YQ22 under laboratory conditions was determined. The YQ22 strain was obtained from diesel samples from a fuel station in Trujillo, Peru, isolated through serial dilutions on Sabouraud agar, and identified through its morphological characteristics using microscopy and molecular analysis by polymerase chain reaction of the ribosomal DNA internal transcribed spacer (ITS) and 5.8S regions. In the treatment, the effect of pH (5, 6 and 7) and temperature (25°C, 30°C and 35°C) on phenol removal with 2% (v/v) inoculum of Yarrowia lipolytica from 48-hour growth was evaluated. Phenol concentration was measured by high-performance liquid chromatography. A maximum phenol removal percentage of 61.18 was obtained for YQ22 at 30°C, pH 5 and 120 rpm during 48 hours. These findings demonstrate the ability of Yarrowia lipolytica to remove phenol and suggest its potential use in the field of bioremediation of phenolic compounds and their derivatives.

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Section: Phenotypic and Molecular Characterizationmentioning

confidence: 99%

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

Baldera-Saavedra,

Quiñones-Cerna,

Sánchez-Vásquez

et al. 2024

EREM

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“…The isolated yeast culture was identified through molecular analysis with the use of a Polymerase Chain Reaction (PCR) of the ITS1 e ITS2 (subunit 5.8S rRNA), according to the methodology of Baldera et al (2022). The DNA was extracted from a highly pure 48 h culture (Nucleic acids extraction and purification kit, innuPREP DNA Kit, Analytik Jena).…”

Section: Isolation and Characterization Of Rhodotorula Mucilaginosamentioning

confidence: 99%

Optimization of Total Carotenoid Production by Rhodotorula mucilaginosa from Artichoke Agroindustrial Waste Using Response Surface Methodology

Rodríguez

Quiñones

Castillo

et al. 2023

EREM

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The carotenoids have vast medical, industrial, dietary, and pharmaceutical importance due to their provitamin A precursor, immunomodulator, antioxidant and photoprotective activity. The purpose of the research was to optimize the production of carotenoids using Rhodotorula mucilaginosa from artichoke agroindustrial waste as a low-cost substrate. The artichokes bracts waste was bleached by sodium hypochlorite (NaClO 2%) and was characterized through whiteness index and FTIR. The bleached artichoke waste (BABW) used in the fermentation went through acid hydrolysis, applying 8% of the bleached artichokes residue and sulfuric acid (2.5%) for 1 h at 90°C, obtaining a greater reduced sugars content at 3.1 g/L. Rhodotorula mucilaginosa was isolated and molecularly identified. The production of carotenoids from a culture media based on hydrolyzed BABW, peptone (0.5%), yeast extract (0.1%) and sodium chloride (0.5%) was evaluated at different conditions of pH (5–8) and agitation speed (80–160 rpm) applying the surface response methodology by a rotational central compound design. The best carotenoids performance obtained had 2968.95 µg/L VVC and 1228.53 µg/g TFC at pH 5, 120 rpm and 30°C for 72 h. The chemical characterization of the extracted carotenoids was confirmed by UV-VIS and Raman spectroscopy methods. The results suggest that Rhodotorula mucilaginosa is capable of producing carotenoids from artichoke waste fermentation, providing a low-cost and sustainable alternative route for use in the global market.

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Bioelectroremediation of hexadecane in electrical cells containing Aspergillus niger immobilized in alginate

Cited by 2 publications

References 22 publications

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

Isolation and Characterization of Yarrowia lipolytica YQ22 from Diesel Samples for Phenol Biodegradation

Optimization of Total Carotenoid Production by Rhodotorula mucilaginosa from Artichoke Agroindustrial Waste Using Response Surface Methodology

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