Bioenergetics of photobiomodulated osteoblast mitochondrial cells derived from human pulp stem cells: systematic review

Sleep, Simone L.; Skelly, Deanne; Love, Robert M.; George, Roy

doi:10.1007/s10103-021-03439-2

Cited by 9 publications

(6 citation statements)

References 39 publications

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“… 31 Additionally, a study explored the positive bioenergetic effects of PBM on the mitochondria of osteoblasts among dental pulp stem cells in humans. 32 …”

Section: Resultsmentioning

confidence: 99%

A systematic review of trends in photobiomodulation in dentistry between 2018 and 2022: advances and investigative agenda

Rodriguez Salazar,

Málaga Rivera,

Laynes Effio

et al. 2023

F1000Res

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Background Photobiomodulation (PBM) involves laser therapy utilized in medical sciences to modulate biological processes acting as a palliative and immune response-enhancing treatment. This study conducts a comprehensive bibliometric analysis to explore current trends in PBM-related scientific production, encompassing publications, citations, impact, keywords and clusters. Additionally, it aims to predict future research trends in this domain. Methods The data for this quantitative and qualitative bibliometric analysis were obtained from 608 scientific documents retrieved in November 2022, with 123 sourced from Web of Science and 485 from Scopus, Utilizing Excel, the data was processed in Excel to extract essencial information. Productivity and impact were evaluated for eligibility, and VOSviewer aided in determining associativity for the bibliometric analysis. Results The findings of this study demostrate that the scientific production related to PBM adheres to a growth power law, exhibiting characteristics of both exponential and linear phases. Notably, recent research trends emphasize critical concepts such as laser therapy, orthodontics, and dental pulp stem cells. Particularly significant is the burgeoning interest in utilizing PBM within dentistry as a complementary alternative to existing protocols. Conclusions PBM stands as a promising laser therapy within medical applications. Through a detailed bibliometric analysis, this study underscores the increasing significance of PBM, especially within the realm of dental treatments. These insights offer a glimpse into the evolving landscape of PBM research and provide valuable guidance for potential future directions of study.

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“… 31 Additionally, a study explored the positive bioenergetic effects of PBM on the mitochondria of osteoblasts among dental pulp stem cells in humans. 32 …”

Section: Resultsmentioning

confidence: 99%

A systematic review of trends in photobiomodulation in dentistry between 2018 and 2022: advances and investigative agenda

Rodriguez Salazar,

Málaga Rivera,

Laynes Effio

et al. 2023

F1000Res

View full text Add to dashboard Cite

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“…To correlate MMP and the role of mitochondria in cell coupling to stress, we also analyzed mitochondrial fusion, which is considered a protective strategy helping to maintain energy output [ 20 ]. Mitochondria are renowned red photon light photoreceptors, and a recent systematic review defined PBM parameters on mitochondrial bioenergetics in different cell types very well [ 21 ]. Most studies have shown positive cell viability data when exposed to wavelengths from 660 to 940 nm at irradiances in the range of 200–400 μ W/mm 2 , for times ranging from 10 to 60 s. The corresponding energy delivery runs from a minimum of 2 mJ/mm 2 to a maximum of 12 mJ/mm 2 .…”

Section: Discussionmentioning

confidence: 99%

Photobiomodulation at Defined Wavelengths Regulates Mitochondrial Membrane Potential and Redox Balance in Skin Fibroblasts

Baldassarro,

Alastra,

Lorenzini

et al. 2023

Oxidative Medicine and Cellular Longevity

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Starting from the discovery of phototherapy in the beginning of the last century, photobiomodulation (PBM) has been defined in late 1960s and, since then, widely described in different in vitro models. Robust evidence indicates that the effect of light exposure on the oxidative state of the cells and on mitochondrial dynamics, suggesting a great therapeutic potential. The translational scale-up of PBM, however, has often given contrasting and confusing results, mainly due to light exposure protocols which fail to adequately control or define factors such as emitting device features, emitted light characteristics, exposure time, cell target, and readouts. In this in vitro study, we describe the effects of a strictly controlled light-emitting diode (LED)-based PBM protocol on human fibroblasts, one of the main cells involved in skin care, regeneration, and repair. We used six emitter probes at different wavelengths (440, 525, 645, 660, 780, and 900 nm) with the same irradiance value of 0.1 mW/cm2, evenly distributed over the entire surface of the cell culture well. The PBM was analyzed by three main readouts: (i) mitochondrial potential (MitoTracker Orange staining), (ii) reactive oxygen species (ROS) production (CellROX staining); and (iii) cell death (nuclear morphology). The assay was also implemented by cell-based high-content screening technology, further increasing the reliability of the data. Different exposure protocols were also tested (one, two, or three subsequent 20 s pulsed exposures at 24 hr intervals), and the 645 nm wavelength and single exposure chosen as the most efficient protocol based on the mitochondrial potential readout, further confirmed by mitochondrial fusion quantification. This protocol was then tested for its potential to prevent H2O2-induced oxidative stress, including modulation of the light wave frequency. Finally, we demonstrated that the controlled PBM induced by the LED light exposure generates a preconditioning stimulation of the mitochondrial potential, which protects the cell from oxidative stress damage.

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“…LLLT stimulates lymphocytes and activates mast cells and the proliferation of various cell types, therefore, acting as an anti-inflammatory and also enhancing wound healing. 19,20 In the present study, 170 wound healing after extraction and 72 ulcer healing was conducted with the help of a diode laser in various pediatric age groups based on the concept of enhanced wound and ulcer healing.…”

Section: Orcidmentioning

confidence: 99%

Demographic Assessment of Laser Therapies in 0–18-year-olds: A Retrospective Study

Mathur¹,

Priyanka²,

Saharia³

2023

Journal of South Asian Association of Pediatric Dentistry

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Materials a n d MethodsThe study was conducted in the Department of Pediatric and Preventive Dentistr y. Ethical clearance was taken from Institutional Ethical Committee, under protocol no. ITSCDSR/IIEC/RP/2021/017. Study DesignA retrospective observational study was designed, and data was collected from practicing Software Orion, being used in the institution, pertaining to laser therapies conducted in the Department of Pediatric and Preventive Dentistry in 0-18-year-old from April 2017 to April 2021. introductionLaser is an advanced technology introduced by Theodore Mainman in 1960, and since then, laser technology has been making inroads into a lot of areas of dentistry due to its unique properties, such as a bloodless field of surgery, less operating time, and less patient discomfort. 1 Laser is a type of electromagnetic energy that is directional, collimated, monochromatic, and coherent (in time and space). Depending on the laser wavelength and optical tissue properties, the laser works accordingly on various types of tissues; diode laser is being popularly used in the field of dentistry with wavelengths of 810-980 nm in the range of visible and invisible near-infrared light. 1,2 Laser has gained importance in medical and dental fields due to its unique properties, such as it provides a bloodless field of surgery, thus providing a clear field of surgery. Apart from this, it promotes fast wound healing with minimal chances of infection; the patient does not feel any vibration and discomfort and requires less operating time, which in turn increases the ease and comfort of the patient.Over the years, the application of laser has been explored in various treatment modalities in the field of dentistry like caries detection, cavity preparation, fluoride activation, pit and fissure application, wound healing, root canal disinfection, crown exposure, desensitization, ulcer healing, crown lengthening, bleaching of teeth, gingivectomy, frenectomy, pulpotomy, direct pulp capping, indirect pulp capping, etc. 3 The objective of this study was to assess the total number of procedures performed with diode lasers and their wide range of

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Bioenergetics of photobiomodulated osteoblast mitochondrial cells derived from human pulp stem cells: systematic review

Cited by 9 publications

References 39 publications

A systematic review of trends in photobiomodulation in dentistry between 2018 and 2022: advances and investigative agenda

A systematic review of trends in photobiomodulation in dentistry between 2018 and 2022: advances and investigative agenda

Photobiomodulation at Defined Wavelengths Regulates Mitochondrial Membrane Potential and Redox Balance in Skin Fibroblasts

Demographic Assessment of Laser Therapies in 0–18-year-olds: A Retrospective Study

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