Bioengineering and Application of Novel Glucose Polymers

Fujii, Kazutoshi; Takata, Hitoshi; Yanase, Michiyo; Terada, Yukimasa; Ohdan, Kohji; Takaha, Takeshi; Okada, Shigetaka; Kuriki, Takashi

doi:10.1080/10242420310001614379

Cited by 102 publications

(44 citation statements)

References 20 publications

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“…According to the structural similarities, 4 GTs are categorized into 5 types including CGTase, D enzyme, amylomaltase, glycogen debranching enzyme (GDE, EC 3.2.1.33 EC 2.4.1.25), and 4 GTs mainly found in archaea. 3,4) Various enzymes belonging to the 4 GT group also catalyze the cyclization reaction and produce cyclic 1,4 glucan, as summarized in Table 1. These results suggest that the cyclization reaction is not a special activity found in a particular enzyme, but a common reaction found in 4 GTs.…”

Section: Improvement Of Amylomaltase From Thermus Aquaticus By Randommentioning

confidence: 99%

Improvement of Amylomaltase from Thermus aquaticus by Random and Saturation Mutageneses

et al. 2005

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Section: Improvement Of Amylomaltase From Thermus Aquaticus By Randommentioning

confidence: 99%

Improvement of Amylomaltase from Thermus aquaticus by Random and Saturation Mutageneses

et al. 2005

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“…The type of cross-linking structure often determines properties of the hydrogels. Because amylose can enzymatically be prepared and hydrolyzed by the phosphorylase and amylase catalyses, respectively [17][18][19][20][21][22]49], hydrogels with cross-linking structures based on amylose have a possibility for enzymatic disruption and reproduction behaviors by two enzyme-catalyzed reactions, i.e., the amylase-catalyzed hydrolysis of amylose and the formation of amylose by the phosphorylase-catalyzed polymerization. Therefore, the preparation of hydrogels through the formation of inclusion complexes of amylose in the vine-twining polymerization was investigated [50].…”

Section: Preparation Of Hydrogels Through Formation Of Inclusion Compmentioning

confidence: 99%

“…In the glycosylation, a glucose unit is transferred from G-1-P to a non-reducing end of the primer to form α-(14)-glycosidic linkage. When the excess molar ratio of G-1-P to the primer is present in the reaction system, the successive glycosylations occur as a propagation of polymerization to produce the α-(14)-glucan chain, i.e., amylose [17][18][19][20][21][22]. Because the phosphorylase-catalyzed polymerization proceeds analogously to a living polymerization, the molecular weight of the produced amylose has a narrow distribution (M w /M n < 1.2) and can be controlled by the G-1-P/primer feed molar ratios [4].…”

Section: Introductionmentioning

confidence: 99%

Preparation and Applications of Amylose Supramolecules by Means of Phosphorylase-Catalyzed Enzymatic Polymerization

Kadokawa

2012

Polymers

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This paper reviews preparation and applications of amylose supramolecules by means of phosphorylase-catalyzed enzymatic polymerization. When the enzymatic polymerization of α-D-glucose 1-phosphate (G-1-P) as a monomer was carried out in the presence of poly(tetrahydrofuran) (PTHF) of a hydrophobic polyether as a guest polymer, the supramolecule, i.e., an amylose-PTHF inclusion complex, was formed in the process of polymerization. Because the representation of propagation in the polymerization is similar to the way that vines of plants grow twining around rods, this polymerization method for the preparation of amylose-polymer inclusion complexes was proposed to be named -vine-twining polymerization‖. Various hydrophobic polyethers, polyesters, poly(ester-ether), and polycarbonates were also employed as the guest polymer in the vine-twining polymerization to produce the corresponding inclusion complexes. To obtain the inclusion complex from a strongly hydrophobic guest polymer, the parallel enzymatic polymerization system was developed as an advanced extension of the vine-twining polymerization. In addition, it was found that amylose selectively includes one side of the guest polymer from a mixture of two resemblant guest polymers, as well as a specific range in molecular weights of the guest PTHF. Amylose also exhibited selective inclusion behavior toward stereoisomers of poly(lactide)s. Moreover, the preparation of hydrogels through the formation of inclusion complexes of amylose in vine-twining polymerization was achieved.

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“…For example, amylopectin analogue, hyper-branched amylose can be synthesized in vitro by combined action of phosphorylase (EC 2.4.1.1) and glycogen branching enzyme (GBE) (EC 2.4.1.18) (Fujii et al, 2003;Yanase, Takaha, & Kuriki, 2006). It was reported that branched polyglucans with the degree of branching of 11% can be synthesized by the tandem action of potato phosphorylase and Deinococcus geothermalis glycogen branching enzyme (Dg GBE) (van der Vlist et al, 2008).…”

Section: Introductionmentioning

confidence: 99%